Development of a prototype wound dressing technology which can detect and report colonization by pathogenic bacteria

Jin Zhou, Thet Naing Tun, Sung-Ha Hong, June D Mercer-Chalmers, Maisem Laabei, Amber E R Young, A Tobias A Jenkins

Research output: Contribution to journalArticlepeer-review

28 Citations (Scopus)
220 Downloads (Pure)

Abstract

A new methodology for detecting the microbiological state of a wound dressing in terms of its colonization with pathogenic bacteria such as Staphylococcus aureus or Pseudomonas aeruginosa has been developed. Here we report how stabilized lipid vesicles containing self-quenched carboxyfluorescein dye are sensitive to lysis only by toxins/virulence factors from P. aeruginosa and S. aureus but not by a non-toxic Escherichia coli species. The development of the stabilized vesicles is discussed and their response to detergent (triton), bacterial toxin (α-hemolysin) and lipases (phospholipase A2). Finally, fabrics with stabilized vesicles attached via plasma deposited maleic anhydride coupling are shown visibly responding to S. aureus (MSSA 476) and P. aeruginosa (PAO1) but not E. coli DH5α in a prototype dressing.
Original languageEnglish
Pages (from-to)67-72
Number of pages6
JournalBiosensors and Bioelectronics
Volume30
Issue number1
DOIs
Publication statusPublished - 15 Dec 2011

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