Abstract
Heme plays a vital role in cell biology and dysregulation of heme levels is implicated in a wide range of diseases. However, monitoring heme levels in biological systems is currently not straightforward. A short synthetic peptide probe containing 7-azatryptophan is shown to bind hemin in vitro with quenching of the azatryptophan fluorescence. This chemical tool can be used to detect the change in free heme induced in human skin cells upon exposure to UVA irradiation.
| Original language | English |
|---|---|
| Pages (from-to) | 467-471 |
| Number of pages | 5 |
| Journal | Organic and Biomolecular Chemistry |
| Volume | 17 |
| Issue number | 3 |
| Early online date | 18 Dec 2018 |
| DOIs | |
| Publication status | Published - 21 Jan 2019 |
Funding
L. D. N. was supported by a studentship from the Biotechnology and Biological Sciences Research Council South-West Doctoral Training Partnership (SWBio, BB/ J014400/1). S. I. P. thanks the European Research Council for the ERC Consolidator Grant O2Sense.
UN SDGs
This output contributes to the following UN Sustainable Development Goals (SDGs)
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SDG 3 Good Health and Well-being
Keywords
- Cell Line
- Fluorescent Dyes/chemical synthesis
- Heme/analysis
- Humans
- Molecular Conformation
- Peptides/chemical synthesis
- Spectrometry, Fluorescence
- Ultraviolet Rays
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