Development of a peptide-based fluorescent probe for biological heme monitoring

Research output: Contribution to journalArticle

Abstract

Heme plays a vital role in cell biology and dysregulation of heme levels is implicated in a wide range of diseases. However, monitoring heme levels in biological systems is currently not straightforward. A short synthetic peptide probe containing 7-azatryptophan is shown to bind hemin in vitro with quenching of the azatryptophan fluorescence. This chemical tool can be used to detect the change in free heme induced in human skin cells upon exposure to UVA irradiation.

LanguageEnglish
Pages467-471
Number of pages5
JournalOrganic and Biomolecular Chemistry
Volume17
Issue number3
Early online date18 Dec 2018
DOIs
StatusPublished - 21 Jan 2019

Keywords

  • Cell Line
  • Fluorescent Dyes/chemical synthesis
  • Heme/analysis
  • Humans
  • Molecular Conformation
  • Peptides/chemical synthesis
  • Spectrometry, Fluorescence
  • Ultraviolet Rays

Cite this

Development of a peptide-based fluorescent probe for biological heme monitoring. / Newton, Laura D.; Pascu, Sofia I.; Tyrrell, Rex M.; Eggleston, Ian M.

In: Organic and Biomolecular Chemistry, Vol. 17, No. 3, 21.01.2019, p. 467-471.

Research output: Contribution to journalArticle

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