Development of a peptide-based fluorescent probe for biological heme monitoring

Laura D. Newton; Sofia I. Pascu; Rex M. Tyrrell; Ian M. Eggleston

doi:10.1039/c8ob02290a

Development of a peptide-based fluorescent probe for biological heme monitoring

Laura D. Newton, Sofia I. Pascu, Rex M. Tyrrell, Ian M. Eggleston

Research output: Contribution to journal › Article › peer-review

13 Citations (SciVal)

Abstract

Heme plays a vital role in cell biology and dysregulation of heme levels is implicated in a wide range of diseases. However, monitoring heme levels in biological systems is currently not straightforward. A short synthetic peptide probe containing 7-azatryptophan is shown to bind hemin in vitro with quenching of the azatryptophan fluorescence. This chemical tool can be used to detect the change in free heme induced in human skin cells upon exposure to UVA irradiation.

Original language	English
Pages (from-to)	467-471
Number of pages	5
Journal	Organic and Biomolecular Chemistry
Volume	17
Issue number	3
Early online date	18 Dec 2018
DOIs	https://doi.org/10.1039/c8ob02290a
Publication status	Published - 21 Jan 2019

Funding

L. D. N. was supported by a studentship from the Biotechnology and Biological Sciences Research Council South-West Doctoral Training Partnership (SWBio, BB/ J014400/1). S. I. P. thanks the European Research Council for the ERC Consolidator Grant O2Sense.

Keywords

Cell Line
Fluorescent Dyes/chemical synthesis
Heme/analysis
Humans
Molecular Conformation
Peptides/chemical synthesis
Spectrometry, Fluorescence
Ultraviolet Rays

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1039/c8ob02290aLicence: CC BY

Cite this

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AU - Pascu, Sofia I.

AU - Tyrrell, Rex M.

AU - Eggleston, Ian M.

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KW - Cell Line

KW - Fluorescent Dyes/chemical synthesis

KW - Heme/analysis

KW - Humans

KW - Molecular Conformation

KW - Peptides/chemical synthesis

KW - Spectrometry, Fluorescence

KW - Ultraviolet Rays

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JO - Organic and Biomolecular Chemistry

JF - Organic and Biomolecular Chemistry

IS - 3

ER -

Development of a peptide-based fluorescent probe for biological heme monitoring

Abstract

Funding

Keywords

UN SDGs

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