Development of a novel laboratory approach for evaluating the adhesion strength of biofilms to human dentine

Jason Bulmer, Y. M. John Chew, Roy George

Research output: Contribution to journalArticlepeer-review

Abstract

Introduction: Biofilms may show varying adherence strengths to dentine. This study quantified the shear force required for the detachment of multispecies biofilm from the dentine using fluid dynamic gauging (FDG) and computation fluid dynamics (CFD). To date this force has not been quantified. Methods: Multispecies biofilms were grown over 3, 7 and 14 days on 2 mm thick dentine sections of human molars (n = 8 per group). The FDG technique with different suction flow rates (100%, 80% and 40%) was used to assess biofilm removal. At maximum suction (100%), the flow rate was 500 mL/min. Digital images of each stained dentine sample were captured (10× magnification) before and after subjecting the samples to the various suction flow rates.

Original languageEnglish
JournalInternational Endodontic Journal
Early online date15 Jan 2025
DOIs
Publication statusPublished - 15 Jan 2025

Data Availability Statement

The data that support the findings of this study are available on request from the corresponding author. The data are not publicly available due to privacy or ethical restrictions.

Funding

This work was partly funded by a grant from the Australian New Zealand Academy of Endodontists (ANZAE).

Keywords

  • bacteria
  • biofilms
  • dentine
  • endodontics
  • microbiology

ASJC Scopus subject areas

  • General Dentistry

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