Development and optimization of a defined medium for aerobic growth of Bacillus stearothermophilus LLD-15

S. A. Amartey; D. J. Leak; B. S. Hartley

doi:10.1007/BF01086315

Development and optimization of a defined medium for aerobic growth of Bacillus stearothermophilus LLD-15

S. A. Amartey, D. J. Leak, B. S. Hartley

Imperial College London

Research output: Contribution to journal › Article › peer-review

Abstract

Methionine, biotin, nicotinic acid and thiamine were found to be absolute requirements for growth of Bacillus stearothermophilus strain LLD-15, an L-lactate dehydrogenase mutant, at 70°C. A defined medium for aerobic growth did not support anaerobic growth. Physiological and subsequent taxonomic studies showed that strain LLD-15 is not a derivative of B. stearothermophilus NCA 1503 as originally thought, but bears some similarity to B. caldotenax.

Original language	English
Pages (from-to)	621-626
Number of pages	6
Journal	Biotechnology Letters
Volume	13
Issue number	9
DOIs	https://doi.org/10.1007/BF01086315
Publication status	Published - 1 Sept 1991

ASJC Scopus subject areas

Microbiology
Applied Microbiology and Biotechnology
Bioengineering
Biotechnology

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abstract = "Methionine, biotin, nicotinic acid and thiamine were found to be absolute requirements for growth of Bacillus stearothermophilus strain LLD-15, an L-lactate dehydrogenase mutant, at 70°C. A defined medium for aerobic growth did not support anaerobic growth. Physiological and subsequent taxonomic studies showed that strain LLD-15 is not a derivative of B. stearothermophilus NCA 1503 as originally thought, but bears some similarity to B. caldotenax.",

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AU - Hartley, B. S.

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N2 - Methionine, biotin, nicotinic acid and thiamine were found to be absolute requirements for growth of Bacillus stearothermophilus strain LLD-15, an L-lactate dehydrogenase mutant, at 70°C. A defined medium for aerobic growth did not support anaerobic growth. Physiological and subsequent taxonomic studies showed that strain LLD-15 is not a derivative of B. stearothermophilus NCA 1503 as originally thought, but bears some similarity to B. caldotenax.

AB - Methionine, biotin, nicotinic acid and thiamine were found to be absolute requirements for growth of Bacillus stearothermophilus strain LLD-15, an L-lactate dehydrogenase mutant, at 70°C. A defined medium for aerobic growth did not support anaerobic growth. Physiological and subsequent taxonomic studies showed that strain LLD-15 is not a derivative of B. stearothermophilus NCA 1503 as originally thought, but bears some similarity to B. caldotenax.

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DO - 10.1007/BF01086315

M3 - Article

AN - SCOPUS:0025784988

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JO - Biotechnology Letters

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Development and optimization of a defined medium for aerobic growth of Bacillus stearothermophilus LLD-15

Abstract

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