Methionine, biotin, nicotinic acid and thiamine were found to be absolute requirements for growth of Bacillus stearothermophilus strain LLD-15, an L-lactate dehydrogenase mutant, at 70°C. A defined medium for aerobic growth did not support anaerobic growth. Physiological and subsequent taxonomic studies showed that strain LLD-15 is not a derivative of B. stearothermophilus NCA 1503 as originally thought, but bears some similarity to B. caldotenax.
ASJC Scopus subject areas
- Applied Microbiology and Biotechnology