Development and optimization of a defined medium for aerobic growth of Bacillus stearothermophilus LLD-15

S. A. Amartey, D. J. Leak, B. S. Hartley

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Methionine, biotin, nicotinic acid and thiamine were found to be absolute requirements for growth of Bacillus stearothermophilus strain LLD-15, an L-lactate dehydrogenase mutant, at 70°C. A defined medium for aerobic growth did not support anaerobic growth. Physiological and subsequent taxonomic studies showed that strain LLD-15 is not a derivative of B. stearothermophilus NCA 1503 as originally thought, but bears some similarity to B. caldotenax.

Original languageEnglish
Pages (from-to)621-626
Number of pages6
JournalBiotechnology Letters
Issue number9
Publication statusPublished - 1 Sep 1991

ASJC Scopus subject areas

  • Microbiology
  • Applied Microbiology and Biotechnology
  • Bioengineering
  • Biotechnology

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