Development and evaluation of a tentacle affinity adsorbent

I A Gyepi-Garbrah, R Eisenthal, A Plant, J Hubble

Research output: Contribution to journalArticle

Abstract

A mech. stable tentacle affinity support has been produced by coating polystyrene beads with polyvinyl alc., coupling dextran tentacles, and subsequently attaching Cibacron blue as a biomimetic ligand. Binding studies using lactate dehydrogenase allowed the effects of ligand d. on binding kinetics and capacity to be detd. Increasing dye loading leads to a decrease in binding capacity but an increase in affinity. The kinetics of binding of lactate dehydrogenase to the tentacle adsorbent showed an operational rate const. that was four times greater than that of obtained with a porous beaded support of similar diam. The tentacle adsorbent had a max. binding capacity approx. 25% of that for the corresponding porous beaded support. Packed bed studies of the flow/pressure drop relationship suggest that the tentacle support can be used over a wider range of flow conditions and could be capable of greater productivity than comparable porous materials. [on SciFinder (R)]
Original languageEnglish
Pages (from-to)195-206
Number of pages12
JournalInternational Journal of Bio-Chromatography
Volume6
Issue number3
Publication statusPublished - 2001

Keywords

  • Affinity chromatography
  • tentacle affinity adsorbent
  • Reaction kinetics (tentacle affinity adsorbent)
  • Adsorbents
  • Porous materials
  • Pressure drop

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