Determining the genetic structure of the natural population of Staphylococcus aureus: A comparison of multilocus sequence typing with pulsed-field gel electrophoresis, randomly amplified polymorphic DNA analysis, and phage typing

H Grundmann; S Hori; M C Enright; C Webster; A Tami; E J Feil; T Pitt

doi:10.1128/jcm.40.12.4544-4546.2002

Determining the genetic structure of the natural population of Staphylococcus aureus: A comparison of multilocus sequence typing with pulsed-field gel electrophoresis, randomly amplified polymorphic DNA analysis, and phage typing

H Grundmann, S Hori, M C Enright, C Webster, A Tami, E J Feil, T Pitt

Department of Life Sciences

Research output: Contribution to journal › Article › peer-review

102 Citations (SciVal)

Abstract

We used a sample of Staphylococcus aureus strains that are carried by humans and that are representative of the natural population of S. aureus strains in order to assess the value of multilocus sequence typing (MLST), pulsed-field gel electrophoresis, randomly amplified polymorphic DNA analysis, and phage typing as epidemiological tools. Only MLST was able to define clonal complexes unambiguously. All DNA-based typing approaches achieved a high degree of agreement, implying phylogenetic concordance, but predicted epidemiological associations with variable accuracy.

Original language	English
Pages (from-to)	4544-4546
Number of pages	3
Journal	Journal of Clinical Microbiology
Volume	40
Issue number	12
DOIs	https://doi.org/10.1128/jcm.40.12.4544-4546.2002
Publication status	Published - 2002

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Grundmann, H., Hori, S., Enright, M. C., Webster, C., Tami, A., Feil, E. J., & Pitt, T. (2002). Determining the genetic structure of the natural population of Staphylococcus aureus: A comparison of multilocus sequence typing with pulsed-field gel electrophoresis, randomly amplified polymorphic DNA analysis, and phage typing. Journal of Clinical Microbiology, 40(12), 4544-4546. https://doi.org/10.1128/jcm.40.12.4544-4546.2002

Determining the genetic structure of the natural population of Staphylococcus aureus: A comparison of multilocus sequence typing with pulsed-field gel electrophoresis, randomly amplified polymorphic DNA analysis, and phage typing. / Grundmann, H; Hori, S; Enright, M C et al.

In: Journal of Clinical Microbiology, Vol. 40, No. 12, 2002, p. 4544-4546.

Research output: Contribution to journal › Article › peer-review

Grundmann, H, Hori, S, Enright, MC, Webster, C, Tami, A, Feil, EJ & Pitt, T 2002, 'Determining the genetic structure of the natural population of Staphylococcus aureus: A comparison of multilocus sequence typing with pulsed-field gel electrophoresis, randomly amplified polymorphic DNA analysis, and phage typing', Journal of Clinical Microbiology, vol. 40, no. 12, pp. 4544-4546. https://doi.org/10.1128/jcm.40.12.4544-4546.2002

Grundmann H, Hori S, Enright MC, Webster C, Tami A, Feil EJ et al. Determining the genetic structure of the natural population of Staphylococcus aureus: A comparison of multilocus sequence typing with pulsed-field gel electrophoresis, randomly amplified polymorphic DNA analysis, and phage typing. Journal of Clinical Microbiology. 2002;40(12):4544-4546. doi: 10.1128/jcm.40.12.4544-4546.2002

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