Detection of a specific fungal pathogen

Hefni Rusli Mohd; Abu Seman Idris; M. Cooper Richard; E Wheals Alan

Detection of a specific fungal pathogen

Hefni Rusli Mohd (Inventor)
, Abu Seman Idris (Inventor)
, M. Cooper Richard (Inventor)
, E Wheals Alan (Inventor)

Department of Life Sciences

Malaysian Palm Oil Board

Research output: Patent

Abstract

The invention relates to methods suitable for detecting Fusarium oxysporum f. sp. elaeidis relying on DNA primers that are designed to a virulence factor gene unique to Fusarium oxysporum f. sp. elaeidis, wherein the sample is grown on Czapek-Dox Iprodione Dichloran medium (CZID) medium, DNA is extracted, and the DNA product is visualised. Preferably, the virulence factor gene is oxido-reductase ORX1. Preferably, Fusarium oxysporum f. sp. elaeidis is detected in seed fragments or pollen samples of plants by polymerase chain reaction (PCR) using primer pair ORF F1/ORF R1.

Original language	English
Patent number	GB2527728
IPC	C12Q1/04,C12Q1/26,C12Q1/68
Priority date	18/02/14
Publication status	Published - 6 Jan 2016

Cite this

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abstract = "The invention relates to methods suitable for detecting Fusarium oxysporum f. sp. elaeidis relying on DNA primers that are designed to a virulence factor gene unique to Fusarium oxysporum f. sp. elaeidis, wherein the sample is grown on Czapek-Dox Iprodione Dichloran medium (CZID) medium, DNA is extracted, and the DNA product is visualised. Preferably, the virulence factor gene is oxido-reductase ORX1. Preferably, Fusarium oxysporum f. sp. elaeidis is detected in seed fragments or pollen samples of plants by polymerase chain reaction (PCR) using primer pair ORF F1/ORF R1.",

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T1 - Detection of a specific fungal pathogen

AU - Mohd, Hefni Rusli

AU - Idris, Abu Seman

AU - Richard, M. Cooper

AU - Alan, E Wheals

PY - 2016/1/6

Y1 - 2016/1/6

N2 - The invention relates to methods suitable for detecting Fusarium oxysporum f. sp. elaeidis relying on DNA primers that are designed to a virulence factor gene unique to Fusarium oxysporum f. sp. elaeidis, wherein the sample is grown on Czapek-Dox Iprodione Dichloran medium (CZID) medium, DNA is extracted, and the DNA product is visualised. Preferably, the virulence factor gene is oxido-reductase ORX1. Preferably, Fusarium oxysporum f. sp. elaeidis is detected in seed fragments or pollen samples of plants by polymerase chain reaction (PCR) using primer pair ORF F1/ORF R1.

AB - The invention relates to methods suitable for detecting Fusarium oxysporum f. sp. elaeidis relying on DNA primers that are designed to a virulence factor gene unique to Fusarium oxysporum f. sp. elaeidis, wherein the sample is grown on Czapek-Dox Iprodione Dichloran medium (CZID) medium, DNA is extracted, and the DNA product is visualised. Preferably, the virulence factor gene is oxido-reductase ORX1. Preferably, Fusarium oxysporum f. sp. elaeidis is detected in seed fragments or pollen samples of plants by polymerase chain reaction (PCR) using primer pair ORF F1/ORF R1.

UR - https://worldwide.espacenet.com/patent/search/family/050440348/publication/GB2527728A?q=pn%3DCR20150081A

M3 - Patent

M1 - GB2527728

ER -

Detection of a specific fungal pathogen

Abstract

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