Designing Zn(II) and Cu(II) derivatives as probes for in vitro fluorescence imaging

Sofia I Pascu; P A Waghorn; T D Conry; H M Betts; J R Dilworth; G C Churchill; T Pokrovska; M Christlieb; F I Aigbirhio; J E Warren

doi:10.1039/b705227h

Designing Zn(II) and Cu(II) derivatives as probes for in vitro fluorescence imaging

Sofia I Pascu, P A Waghorn, T D Conry, H M Betts, J R Dilworth, G C Churchill, T Pokrovska, M Christlieb, F I Aigbirhio, J E Warren

Department of Chemistry

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Abstract

New M( II) bis(thiosemicarbazonato) complexes ( M = Ni( II), Cu( II) and Zn( II)) featuring allyl groups at the exocyclic nitrogens have been synthesised. The complexes were characterised in solution by spectroscopic methods and their solid state structures determined by single crystal X-ray diffraction using synchrotron radiation. The Zn( II) complex was found to be intrinsically fluorescent and soluble in biocompatible media. The uptake of this Zn( II) complex in HeLa, MCF-7 and IGROV cancer cells was monitored by fluorescence microscopies ( epi- and confocal fluorescence imaging). The radiolabelling to Cu-64( II) bis( thiosemicarbazonato) complex was performed cleanly by transmetallation from the corresponding Zn( II) species using Cu-64( OAc)(2).

Original language	English
Pages (from-to)	4988-4997
Number of pages	10
Journal	Dalton Transactions
Volume	2007
Issue number	43
DOIs	https://doi.org/10.1039/b705227h
Publication status	Published - 2007

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title = "Designing Zn(II) and Cu(II) derivatives as probes for in vitro fluorescence imaging",

abstract = "New M( II) bis(thiosemicarbazonato) complexes ( M = Ni( II), Cu( II) and Zn( II)) featuring allyl groups at the exocyclic nitrogens have been synthesised. The complexes were characterised in solution by spectroscopic methods and their solid state structures determined by single crystal X-ray diffraction using synchrotron radiation. The Zn( II) complex was found to be intrinsically fluorescent and soluble in biocompatible media. The uptake of this Zn( II) complex in HeLa, MCF-7 and IGROV cancer cells was monitored by fluorescence microscopies ( epi- and confocal fluorescence imaging). The radiolabelling to Cu-64( II) bis( thiosemicarbazonato) complex was performed cleanly by transmetallation from the corresponding Zn( II) species using Cu-64( OAc)(2).",

author = "Pascu, {Sofia I} and Waghorn, {P A} and Conry, {T D} and Betts, {H M} and Dilworth, {J R} and Churchill, {G C} and T Pokrovska and M Christlieb and Aigbirhio, {F I} and Warren, {J E}",

year = "2007",

doi = "10.1039/b705227h",

language = "English",

volume = "2007",

pages = "4988--4997",

journal = "Dalton Transactions",

issn = "1477-9226",

publisher = "Royal Society of Chemistry",

number = "43",

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TY - JOUR

T1 - Designing Zn(II) and Cu(II) derivatives as probes for in vitro fluorescence imaging

AU - Pascu, Sofia I

AU - Waghorn, P A

AU - Conry, T D

AU - Betts, H M

AU - Dilworth, J R

AU - Churchill, G C

AU - Pokrovska, T

AU - Christlieb, M

AU - Aigbirhio, F I

AU - Warren, J E

PY - 2007

Y1 - 2007

N2 - New M( II) bis(thiosemicarbazonato) complexes ( M = Ni( II), Cu( II) and Zn( II)) featuring allyl groups at the exocyclic nitrogens have been synthesised. The complexes were characterised in solution by spectroscopic methods and their solid state structures determined by single crystal X-ray diffraction using synchrotron radiation. The Zn( II) complex was found to be intrinsically fluorescent and soluble in biocompatible media. The uptake of this Zn( II) complex in HeLa, MCF-7 and IGROV cancer cells was monitored by fluorescence microscopies ( epi- and confocal fluorescence imaging). The radiolabelling to Cu-64( II) bis( thiosemicarbazonato) complex was performed cleanly by transmetallation from the corresponding Zn( II) species using Cu-64( OAc)(2).

AB - New M( II) bis(thiosemicarbazonato) complexes ( M = Ni( II), Cu( II) and Zn( II)) featuring allyl groups at the exocyclic nitrogens have been synthesised. The complexes were characterised in solution by spectroscopic methods and their solid state structures determined by single crystal X-ray diffraction using synchrotron radiation. The Zn( II) complex was found to be intrinsically fluorescent and soluble in biocompatible media. The uptake of this Zn( II) complex in HeLa, MCF-7 and IGROV cancer cells was monitored by fluorescence microscopies ( epi- and confocal fluorescence imaging). The radiolabelling to Cu-64( II) bis( thiosemicarbazonato) complex was performed cleanly by transmetallation from the corresponding Zn( II) species using Cu-64( OAc)(2).

UR - http://dx.doi.org/10.1039/b705227h

U2 - 10.1039/b705227h

DO - 10.1039/b705227h

M3 - Article

SN - 1477-9226

VL - 2007

SP - 4988

EP - 4997

JO - Dalton Transactions

JF - Dalton Transactions

IS - 43

ER -

Designing Zn(II) and Cu(II) derivatives as probes for in vitro fluorescence imaging

Abstract

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