TY - JOUR
T1 - Description of klebsiella spallanzanii sp. Nov. and of klebsiella pasteurii sp. nov.
AU - Merla, Cristina
AU - Rodrigues, Carla
AU - Passet, Virginie
AU - Corbella, Marta
AU - Thorpe, Harry A.
AU - Kallonen, Teemu V.S.
AU - Zong, Zhiyong
AU - Marone, Piero
AU - Bandi, Claudio
AU - Sassera, Davide
AU - Corander, Jukka
AU - Feil, Edward J.
AU - Brisse, Sylvain
N1 - Copyright © 2019 Merla, Rodrigues, Passet, Corbella, Thorpe, Kallonen, Zong, Marone, Bandi, Sassera, Corander, Feil and Brisse.
PY - 2019/10/25
Y1 - 2019/10/25
N2 - Klebsiella oxytoca causes opportunistic human infections and post-antibiotic haemorrhagic diarrhea. This Enterobacteriaceae species is genetically heterogeneous and is currently subdivided into seven phylogroups (Ko1 to Ko4 and Ko6 to Ko8). Here we investigated the taxonomic status of phylogroups Ko3 and Ko4. Genomic sequence-based phylogenetic analyses demonstrate that Ko3 and Ko4 formed well-defined sequence clusters related to, but distinct from, Klebsiella michiganensis (Ko1), K. oxytoca (Ko2), K. huaxiensis (Ko8), and K. grimontii (Ko6). The average nucleotide identity (ANI) of Ko3 and Ko4 were 90.7% with K. huaxiensis and 95.5% with K. grimontii, respectively. In addition, three strains of K. huaxiensis, a species so far described based on a single strain from a urinary tract infection patient in China, were isolated from cattle and human feces. Biochemical and MALDI-ToF mass spectrometry analysis allowed differentiating Ko3, Ko4, and Ko8 from the other K. oxytoca species. Based on these results, we propose the names Klebsiella spallanzanii for the Ko3 phylogroup, with SPARK_775_C1T (CIP 111695T and DSM 109531T ) as type strain, and Klebsiella pasteurii for Ko4, with SPARK_836_C1T (CIP 111696T and DSM 109530T ) as type strain. Strains of K. spallanzanii were isolated from human urine, cow feces, and farm surfaces, while strains of K. pasteurii were found in fecal carriage from humans, cows, and turtles.
AB - Klebsiella oxytoca causes opportunistic human infections and post-antibiotic haemorrhagic diarrhea. This Enterobacteriaceae species is genetically heterogeneous and is currently subdivided into seven phylogroups (Ko1 to Ko4 and Ko6 to Ko8). Here we investigated the taxonomic status of phylogroups Ko3 and Ko4. Genomic sequence-based phylogenetic analyses demonstrate that Ko3 and Ko4 formed well-defined sequence clusters related to, but distinct from, Klebsiella michiganensis (Ko1), K. oxytoca (Ko2), K. huaxiensis (Ko8), and K. grimontii (Ko6). The average nucleotide identity (ANI) of Ko3 and Ko4 were 90.7% with K. huaxiensis and 95.5% with K. grimontii, respectively. In addition, three strains of K. huaxiensis, a species so far described based on a single strain from a urinary tract infection patient in China, were isolated from cattle and human feces. Biochemical and MALDI-ToF mass spectrometry analysis allowed differentiating Ko3, Ko4, and Ko8 from the other K. oxytoca species. Based on these results, we propose the names Klebsiella spallanzanii for the Ko3 phylogroup, with SPARK_775_C1T (CIP 111695T and DSM 109531T ) as type strain, and Klebsiella pasteurii for Ko4, with SPARK_836_C1T (CIP 111696T and DSM 109530T ) as type strain. Strains of K. spallanzanii were isolated from human urine, cow feces, and farm surfaces, while strains of K. pasteurii were found in fecal carriage from humans, cows, and turtles.
KW - Bla
KW - Genome sequencing
KW - Klebsiella oxytoca complex
KW - MALDI-ToF mass spectrometry
KW - Phylogeny
KW - Taxonomy
UR - http://www.scopus.com/inward/record.url?scp=85074590400&partnerID=8YFLogxK
U2 - 10.3389/fmicb.2019.02360
DO - 10.3389/fmicb.2019.02360
M3 - Article
C2 - 31708881
AN - SCOPUS:85074590400
VL - 10
SP - 1
EP - 9
JO - Frontiers in Microbiology
JF - Frontiers in Microbiology
SN - 1664-302x
M1 - 2360
ER -
