Description of klebsiella spallanzanii sp. Nov. and of klebsiella pasteurii sp. nov.

Cristina Merla; Carla Rodrigues; Virginie Passet; Marta Corbella; Harry A. Thorpe; Teemu V.S. Kallonen; Zhiyong Zong; Piero Marone; Claudio Bandi; Davide Sassera; Jukka Corander; Edward J. Feil; Sylvain Brisse

doi:10.3389/fmicb.2019.02360

Description of klebsiella spallanzanii sp. Nov. and of klebsiella pasteurii sp. nov.

Cristina Merla, Carla Rodrigues, Virginie Passet, Marta Corbella, Harry A. Thorpe, Teemu V.S. Kallonen, Zhiyong Zong, Piero Marone, Claudio Bandi, Davide Sassera, Jukka Corander, Edward J. Feil, Sylvain Brisse

Department of Life Sciences

Research output: Contribution to journal › Article › peer-review

17 Citations (SciVal)

Abstract

Klebsiella oxytoca causes opportunistic human infections and post-antibiotic haemorrhagic diarrhea. This Enterobacteriaceae species is genetically heterogeneous and is currently subdivided into seven phylogroups (Ko1 to Ko4 and Ko6 to Ko8). Here we investigated the taxonomic status of phylogroups Ko3 and Ko4. Genomic sequence-based phylogenetic analyses demonstrate that Ko3 and Ko4 formed well-defined sequence clusters related to, but distinct from, Klebsiella michiganensis (Ko1), K. oxytoca (Ko2), K. huaxiensis (Ko8), and K. grimontii (Ko6). The average nucleotide identity (ANI) of Ko3 and Ko4 were 90.7% with K. huaxiensis and 95.5% with K. grimontii, respectively. In addition, three strains of K. huaxiensis, a species so far described based on a single strain from a urinary tract infection patient in China, were isolated from cattle and human feces. Biochemical and MALDI-ToF mass spectrometry analysis allowed differentiating Ko3, Ko4, and Ko8 from the other K. oxytoca species. Based on these results, we propose the names Klebsiella spallanzanii for the Ko3 phylogroup, with SPARK_775_C1^T (CIP 111695^T and DSM 109531^T ) as type strain, and Klebsiella pasteurii for Ko4, with SPARK_836_C1^T (CIP 111696^T and DSM 109530^T ) as type strain. Strains of K. spallanzanii were isolated from human urine, cow feces, and farm surfaces, while strains of K. pasteurii were found in fecal carriage from humans, cows, and turtles.

Original language	English
Article number	2360
Pages (from-to)	1-9
Number of pages	9
Journal	Frontiers in Microbiology
Volume	10
DOIs	https://doi.org/10.3389/fmicb.2019.02360
Publication status	Published - 25 Oct 2019

Keywords

Bla
Genome sequencing
Klebsiella oxytoca complex
MALDI-ToF mass spectrometry
Phylogeny
Taxonomy

ASJC Scopus subject areas

Microbiology
Microbiology (medical)

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.3389/fmicb.2019.02360Licence: CC BY

Cite this

@article{b4031798c54a4569835e85be29fdb64f,

title = "Description of klebsiella spallanzanii sp. Nov. and of klebsiella pasteurii sp. nov.",

abstract = "Klebsiella oxytoca causes opportunistic human infections and post-antibiotic haemorrhagic diarrhea. This Enterobacteriaceae species is genetically heterogeneous and is currently subdivided into seven phylogroups (Ko1 to Ko4 and Ko6 to Ko8). Here we investigated the taxonomic status of phylogroups Ko3 and Ko4. Genomic sequence-based phylogenetic analyses demonstrate that Ko3 and Ko4 formed well-defined sequence clusters related to, but distinct from, Klebsiella michiganensis (Ko1), K. oxytoca (Ko2), K. huaxiensis (Ko8), and K. grimontii (Ko6). The average nucleotide identity (ANI) of Ko3 and Ko4 were 90.7% with K. huaxiensis and 95.5% with K. grimontii, respectively. In addition, three strains of K. huaxiensis, a species so far described based on a single strain from a urinary tract infection patient in China, were isolated from cattle and human feces. Biochemical and MALDI-ToF mass spectrometry analysis allowed differentiating Ko3, Ko4, and Ko8 from the other K. oxytoca species. Based on these results, we propose the names Klebsiella spallanzanii for the Ko3 phylogroup, with SPARK_775_C1T (CIP 111695T and DSM 109531T ) as type strain, and Klebsiella pasteurii for Ko4, with SPARK_836_C1T (CIP 111696T and DSM 109530T ) as type strain. Strains of K. spallanzanii were isolated from human urine, cow feces, and farm surfaces, while strains of K. pasteurii were found in fecal carriage from humans, cows, and turtles.",

keywords = "Bla, Genome sequencing, Klebsiella oxytoca complex, MALDI-ToF mass spectrometry, Phylogeny, Taxonomy",

author = "Cristina Merla and Carla Rodrigues and Virginie Passet and Marta Corbella and Thorpe, {Harry A.} and Kallonen, {Teemu V.S.} and Zhiyong Zong and Piero Marone and Claudio Bandi and Davide Sassera and Jukka Corander and Feil, {Edward J.} and Sylvain Brisse",

note = "Copyright {\textcopyright} 2019 Merla, Rodrigues, Passet, Corbella, Thorpe, Kallonen, Zong, Marone, Bandi, Sassera, Corander, Feil and Brisse.",

year = "2019",

month = oct,

day = "25",

doi = "10.3389/fmicb.2019.02360",

language = "English",

volume = "10",

pages = "1--9",

journal = "Frontiers in Microbiology",

issn = "1664-302x",

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T1 - Description of klebsiella spallanzanii sp. Nov. and of klebsiella pasteurii sp. nov.

AU - Merla, Cristina

AU - Rodrigues, Carla

AU - Passet, Virginie

AU - Corbella, Marta

AU - Thorpe, Harry A.

AU - Kallonen, Teemu V.S.

AU - Zong, Zhiyong

AU - Marone, Piero

AU - Bandi, Claudio

AU - Sassera, Davide

AU - Corander, Jukka

AU - Feil, Edward J.

AU - Brisse, Sylvain

PY - 2019/10/25

Y1 - 2019/10/25

N2 - Klebsiella oxytoca causes opportunistic human infections and post-antibiotic haemorrhagic diarrhea. This Enterobacteriaceae species is genetically heterogeneous and is currently subdivided into seven phylogroups (Ko1 to Ko4 and Ko6 to Ko8). Here we investigated the taxonomic status of phylogroups Ko3 and Ko4. Genomic sequence-based phylogenetic analyses demonstrate that Ko3 and Ko4 formed well-defined sequence clusters related to, but distinct from, Klebsiella michiganensis (Ko1), K. oxytoca (Ko2), K. huaxiensis (Ko8), and K. grimontii (Ko6). The average nucleotide identity (ANI) of Ko3 and Ko4 were 90.7% with K. huaxiensis and 95.5% with K. grimontii, respectively. In addition, three strains of K. huaxiensis, a species so far described based on a single strain from a urinary tract infection patient in China, were isolated from cattle and human feces. Biochemical and MALDI-ToF mass spectrometry analysis allowed differentiating Ko3, Ko4, and Ko8 from the other K. oxytoca species. Based on these results, we propose the names Klebsiella spallanzanii for the Ko3 phylogroup, with SPARK_775_C1T (CIP 111695T and DSM 109531T ) as type strain, and Klebsiella pasteurii for Ko4, with SPARK_836_C1T (CIP 111696T and DSM 109530T ) as type strain. Strains of K. spallanzanii were isolated from human urine, cow feces, and farm surfaces, while strains of K. pasteurii were found in fecal carriage from humans, cows, and turtles.

AB - Klebsiella oxytoca causes opportunistic human infections and post-antibiotic haemorrhagic diarrhea. This Enterobacteriaceae species is genetically heterogeneous and is currently subdivided into seven phylogroups (Ko1 to Ko4 and Ko6 to Ko8). Here we investigated the taxonomic status of phylogroups Ko3 and Ko4. Genomic sequence-based phylogenetic analyses demonstrate that Ko3 and Ko4 formed well-defined sequence clusters related to, but distinct from, Klebsiella michiganensis (Ko1), K. oxytoca (Ko2), K. huaxiensis (Ko8), and K. grimontii (Ko6). The average nucleotide identity (ANI) of Ko3 and Ko4 were 90.7% with K. huaxiensis and 95.5% with K. grimontii, respectively. In addition, three strains of K. huaxiensis, a species so far described based on a single strain from a urinary tract infection patient in China, were isolated from cattle and human feces. Biochemical and MALDI-ToF mass spectrometry analysis allowed differentiating Ko3, Ko4, and Ko8 from the other K. oxytoca species. Based on these results, we propose the names Klebsiella spallanzanii for the Ko3 phylogroup, with SPARK_775_C1T (CIP 111695T and DSM 109531T ) as type strain, and Klebsiella pasteurii for Ko4, with SPARK_836_C1T (CIP 111696T and DSM 109530T ) as type strain. Strains of K. spallanzanii were isolated from human urine, cow feces, and farm surfaces, while strains of K. pasteurii were found in fecal carriage from humans, cows, and turtles.

KW - Bla

KW - Genome sequencing

KW - Klebsiella oxytoca complex

KW - MALDI-ToF mass spectrometry

KW - Phylogeny

KW - Taxonomy

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U2 - 10.3389/fmicb.2019.02360

DO - 10.3389/fmicb.2019.02360

M3 - Article

C2 - 31708881

AN - SCOPUS:85074590400

VL - 10

SP - 1

EP - 9

JO - Frontiers in Microbiology

JF - Frontiers in Microbiology

SN - 1664-302x

M1 - 2360

ER -

Description of klebsiella spallanzanii sp. Nov. and of klebsiella pasteurii sp. nov.

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

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SpARK - The Rates and Routes of Transmission of Multidrug Resistant Klebsiella Clones into the Clinic from Environmental Sources

WGS-aqua: Capacity Building for the Widespread Adoption of Whole Genome Sequencing (WGS) for the Molecular Epidemiology of Aquaculture Pathogens

Cite this

Description of klebsiella spallanzanii sp. Nov. and of klebsiella pasteurii sp. nov.

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

Other files and links

Fingerprint

Projects

SpARK - The Rates and Routes of Transmission of Multidrug Resistant Klebsiella Clones into the Clinic from Environmental Sources

WGS-aqua: Capacity Building for the Widespread Adoption of Whole Genome Sequencing (WGS) for the Molecular Epidemiology of Aquaculture Pathogens

Cite this