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Abstract
Klebsiella oxytoca causes opportunistic human infections and post-antibiotic haemorrhagic diarrhea. This Enterobacteriaceae species is genetically heterogeneous and is currently subdivided into seven phylogroups (Ko1 to Ko4 and Ko6 to Ko8). Here we investigated the taxonomic status of phylogroups Ko3 and Ko4. Genomic sequence-based phylogenetic analyses demonstrate that Ko3 and Ko4 formed well-defined sequence clusters related to, but distinct from, Klebsiella michiganensis (Ko1), K. oxytoca (Ko2), K. huaxiensis (Ko8), and K. grimontii (Ko6). The average nucleotide identity (ANI) of Ko3 and Ko4 were 90.7% with K. huaxiensis and 95.5% with K. grimontii, respectively. In addition, three strains of K. huaxiensis, a species so far described based on a single strain from a urinary tract infection patient in China, were isolated from cattle and human feces. Biochemical and MALDI-ToF mass spectrometry analysis allowed differentiating Ko3, Ko4, and Ko8 from the other K. oxytoca species. Based on these results, we propose the names Klebsiella spallanzanii for the Ko3 phylogroup, with SPARK_775_C1T (CIP 111695T and DSM 109531T ) as type strain, and Klebsiella pasteurii for Ko4, with SPARK_836_C1T (CIP 111696T and DSM 109530T ) as type strain. Strains of K. spallanzanii were isolated from human urine, cow feces, and farm surfaces, while strains of K. pasteurii were found in fecal carriage from humans, cows, and turtles.
Original language | English |
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Article number | 2360 |
Pages (from-to) | 1-9 |
Number of pages | 9 |
Journal | Frontiers in Microbiology |
Volume | 10 |
DOIs | |
Publication status | Published - 25 Oct 2019 |
Bibliographical note
Copyright © 2019 Merla, Rodrigues, Passet, Corbella, Thorpe, Kallonen, Zong, Marone, Bandi, Sassera, Corander, Feil and Brisse.Funding
This work was funded by the SpARK project ?The rates and routes of transmission of multidrug resistant Klebsiella clones and genes into the clinic from environmental sources,? which has received funding under the 2016 JPI-AMR call ?Transmission Dynamics? (MRC reference MR/R00241X/1); and by the French Government?s Investissement d?Avenir program Laboratoire d?Excellence ?Integrative Biology of Emerging Infectious Diseases? (ANR-10-LABX-62-IBEID). CR was financially supported by the MedVetKlebs project, a component of European Joint Programme One Health EP, which has received funding from the European Union?s Horizon 2020 Research and Innovation Programme under Grant Agreement No. 773830. JC was funded by the ERC grant no. 742158 and the Norwegian Research Council JPIAMR grant no. 144501.
Keywords
- Bla
- Genome sequencing
- Klebsiella oxytoca complex
- MALDI-ToF mass spectrometry
- Phylogeny
- Taxonomy
ASJC Scopus subject areas
- Microbiology
- Microbiology (medical)
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- 2 Finished
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SpARK - The Rates and Routes of Transmission of Multidrug Resistant Klebsiella Clones into the Clinic from Environmental Sources
Feil, E. (PI)
1/04/17 → 31/12/20
Project: Research council