Derivation of primary choroid plexus epithelial cells from the mouse

Trevelyan R Menheniott, Marika Charalambous, Andrew Ward

Research output: Chapter in Book/Report/Conference proceedingChapter

11 Citations (Scopus)

Abstract

Choroid plexus epithelial cells form an integral and important part of the barrier between blood and cerebrospinal fluid. Culture of choroid plexus epithelium in vitro has been achieved from several mammalian species and this provides opportunities for the study of choroid plexus development and function, including the capacity of the epithelial cells to control the movement of bioactive molecules, such as novel drug candidates, from the bloodstream to the brain. Here we describe a method for the derivation of primary cell cultures from mouse choroid plexus epithelium, together with characterisation by immunofluorescence using antibodies specific to markers of mature choroid plexus epithelial cells. With this method, relatively pure choroid plexus epithelial cell monolayers are established using the DNA synthesis inhibitor cytosine arabinoside (Ara-C), which is cytotoxic to contaminating cell types such as fibroblasts, but not the epithelial cells. These cells are shown to express the diagnostic choroidal marker, transthyretin (TTR), as well as markers of epithelial cell differentiation and are thus suitable for studies that address the transport and barrier functions of the choroid plexus.

Original languageEnglish
Title of host publicationMouse Cell Culture
Subtitle of host publicationMethods and Protocols
EditorsAndrew Ward, David Tosh
PublisherHumana Press
Pages207-20
Number of pages14
Volume633
ISBN (Electronic)978-1-59745-019-5
ISBN (Print)978-1-58829-772-3
DOIs
Publication statusPublished - 2010

Publication series

NameMethods in Molecular Biology
PublisherHumana Press
Volume633
ISSN (Print)1064-3745

Fingerprint

Choroid Plexus
Epithelial Cells
Cytarabine
Epithelium
Nucleic Acid Synthesis Inhibitors
Prealbumin
Primary Cell Culture
Fluorescent Antibody Technique
Cerebrospinal Fluid
Cell Differentiation
Fibroblasts
Antibodies
Brain
Pharmaceutical Preparations

Keywords

  • Animals
  • Biomarkers
  • Cell Culture Techniques
  • Cell Separation
  • Choroid Plexus
  • Epithelial Cells
  • Fluorescent Antibody Technique, Indirect
  • Humans
  • Mice
  • Journal Article
  • Research Support, Non-U.S. Gov't

Cite this

Menheniott, T. R., Charalambous, M., & Ward, A. (2010). Derivation of primary choroid plexus epithelial cells from the mouse. In A. Ward, & D. Tosh (Eds.), Mouse Cell Culture: Methods and Protocols (Vol. 633, pp. 207-20). (Methods in Molecular Biology; Vol. 633). Humana Press. https://doi.org/10.1007/978-1-59745-019-5_15

Derivation of primary choroid plexus epithelial cells from the mouse. / Menheniott, Trevelyan R; Charalambous, Marika; Ward, Andrew.

Mouse Cell Culture: Methods and Protocols. ed. / Andrew Ward; David Tosh. Vol. 633 Humana Press, 2010. p. 207-20 (Methods in Molecular Biology; Vol. 633).

Research output: Chapter in Book/Report/Conference proceedingChapter

Menheniott, TR, Charalambous, M & Ward, A 2010, Derivation of primary choroid plexus epithelial cells from the mouse. in A Ward & D Tosh (eds), Mouse Cell Culture: Methods and Protocols. vol. 633, Methods in Molecular Biology, vol. 633, Humana Press, pp. 207-20. https://doi.org/10.1007/978-1-59745-019-5_15
Menheniott TR, Charalambous M, Ward A. Derivation of primary choroid plexus epithelial cells from the mouse. In Ward A, Tosh D, editors, Mouse Cell Culture: Methods and Protocols. Vol. 633. Humana Press. 2010. p. 207-20. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-59745-019-5_15
Menheniott, Trevelyan R ; Charalambous, Marika ; Ward, Andrew. / Derivation of primary choroid plexus epithelial cells from the mouse. Mouse Cell Culture: Methods and Protocols. editor / Andrew Ward ; David Tosh. Vol. 633 Humana Press, 2010. pp. 207-20 (Methods in Molecular Biology).
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