The paper describes the development of an electric bicyclc mathematical model and its experimental validation. The modelling procedure has focuscd on the vehicle and the electric motor dynamics. The model has been particularized with respect to an innovative electric bicycle. Preliminary experimental tests have involved the characterization of a novel chain strength sensor and then, different drive conditions have been assigned and the results have been collected in order to carry on the parameter identification. Successively, novel experimental results have been adopted to validate the proposed model. The validation procedure has highlighted the goodness of the adopted approach, allowing to employ the mathematical model for predictive analysis of the electric bicycle performances in simulation environment.