Delivery and quantification of hydrogen peroxide generated via cold atmospheric pressure plasma through biological material

H. J. Hathaway, B. L. Patenall, N. T. Thet, A. C. Sedgwick, G. T. Williams, A. T. A. Jenkins, S. L. Allinson, R. D. Short

Research output: Contribution to journalArticle

Abstract

The ability of plasma-generated hydrogen peroxide (H2O2) to traverse bacterial biofilms and the subsequent fate of the generated H2O2 has been investigated. An in vitro model, comprising a nanoporous membrane impregnated with artificial wound fluid and biofilms of varying maturity was treated with a helium-driven, cold atmospheric pressure plasma (CAP) jet. The concentration of H2O2 generated below the biofilms was quantified. The results showed that the plasma-generated H2O2 interacted significantly with the biofilm, thus exhibiting a reduction in concentration across the underlying nanoporous membrane. Biofilm maturity exhibited a significant effect on the penetration depth of H2O2, suggesting that well established, multilayer biofilms are likely to offer a shielding effect with respect to cells located in the lower layers of the biofilm, thus rendering them less susceptible to plasma disinfection. This may prove clinically significant in the plasma treatment of chronic, deep tissue infections such as diabetic and venous leg ulcers. Our results are discussed in the context of plasma-biofilm interactions, with respect to the fate of the longer lived reactive species generated by CAP, such as H2O2

Original languageEnglish
Article number505203
JournalJournal of Physics D: Applied Physics
Volume52
Issue number50
Early online date17 Sep 2019
DOIs
Publication statusPublished - 9 Oct 2019

Keywords

  • biofilms
  • cold plasma
  • plasma
  • reactive species

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Acoustics and Ultrasonics
  • Surfaces, Coatings and Films

Cite this