Deciphering the complexity of human noncoding promoter-proximal transcriptome

Sarah N Mapelli, Sara Napoli, Giuseppina Pisignano, Ramon Garcia-Escudero, Giuseppina M Carbone, Carlo V Catapano

Research output: Contribution to journalArticle

Abstract

Motivation: Long noncoding RNAs (lncRNAs) have gained increasing relevance in epigenetic regulation and nuclear functional organization. High-throughput sequencing approaches have revealed frequent noncoding transcription in promoter-proximal regions. However, a comprehensive catalogue of promoter-associated RNAs (paRNAs) and an analysis of the possible interactions with neighboring genes and genomic regulatory elements are missing.

Results: Integrating data from multiple cell types and experimental platforms we identified thousands of paRNAs in the human genome. paRNAs are transcribed in both sense and antisense orientation, are mostly non-polyadenylated and retained in the cell nucleus. Transcriptional regulators, epigenetic effectors and activating chromatin marks are enriched in paRNA-positive promoters. Furthermore, paRNA-positive promoters exhibit chromatin signatures of both active promoters and enhancers. Promoters with paRNAs reside preferentially at chromatin loop boundaries, suggesting an involvement in anchor site recognition and chromatin looping. Importantly, these features are independent of the transcriptional state of neighboring genes. Thus, paRNAs may act as cis-regulatory modules with an impact on local recruitment of transcription factors, epigenetic state and chromatin loop organization. This study provides a comprehensive analysis of the promoter-proximal transcriptome and offers novel insights into the roles of paRNAs in epigenetic processes and human diseases.

Availability: Genomic coordinates of predicted paRNAs are available at https://figshare.com: doi.org/10.6084/m9.figshare.4856630.v2.

Supplementary information: Supplementary data, detailed methods and extended discussion are available at Bioinformatics online.
Original languageUndefined/Unknown
Pages (from-to)bty981
JournalBioinformatics
Early online date10 Dec 2018
DOIs
Publication statusE-pub ahead of print - 10 Dec 2018

Cite this

Mapelli, S. N., Napoli, S., Pisignano, G., Garcia-Escudero, R., Carbone, G. M., & Catapano, C. V. (2018). Deciphering the complexity of human noncoding promoter-proximal transcriptome. Bioinformatics , bty981. https://doi.org/10.1093/bioinformatics/bty981

Deciphering the complexity of human noncoding promoter-proximal transcriptome. / Mapelli, Sarah N; Napoli, Sara; Pisignano, Giuseppina; Garcia-Escudero, Ramon; Carbone, Giuseppina M; Catapano, Carlo V.

In: Bioinformatics , 10.12.2018, p. bty981.

Research output: Contribution to journalArticle

Mapelli, Sarah N ; Napoli, Sara ; Pisignano, Giuseppina ; Garcia-Escudero, Ramon ; Carbone, Giuseppina M ; Catapano, Carlo V. / Deciphering the complexity of human noncoding promoter-proximal transcriptome. In: Bioinformatics . 2018 ; pp. bty981.
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