Decellularized grass as a sustainable scaffold for skeletal muscle tissue engineering

Scott Allan; Marianne Ellis; Paul De Bank

doi:10.1002/jbm.a.37241

Decellularized grass as a sustainable scaffold for skeletal muscle tissue engineering

Scott Allan, Marianne Ellis, Paul De Bank

Research output: Contribution to journal › Article › peer-review

48 Citations (SciVal)

387 Downloads (Pure)

Abstract

Scaffold materials suitable for the scale-up and subsequent commercialization of tissue engineered products should ideally be cost effective and accessible. For the in vitro culture of certain adherent cells, synthetic fabrication techniques are often employed to produce micro- or nano-patterned substrates to influence cell attachment, morphology, and alignment via the mechanism of contact guidance. Here we present a natural scaffold, in the form of decellularized amenity grass, which retains its natural striated topography and supports the attachment, proliferation, alignment and differentiation of murine C2C12 myoblasts, without the need for additional functionalization. This presents an inexpensive, sustainable scaffold material and structure for tissue engineering applications capable of influencing cell alignment, a desired property for the culture of skeletal muscle and other anisotropic tissues.

Original language	English
Pages (from-to)	2471-2482
Number of pages	12
Journal	Journal of Biomedical Materials Research - Part A
Volume	109
Issue number	12
Early online date	31 May 2021
DOIs	https://doi.org/10.1002/jbm.a.37241
Publication status	Published - 31 Dec 2021

Bibliographical note

Funding Information:
This work was funded by New Harvest, a 501(c) (3) non‐profit research institute (grant #007) and the EPSRC Centre for Doctoral Training in Sustainable Chemical Technologies (EP/L016354/1). The authors would like to thank the University of Bath for supporting this work, gratefully acknowledge the assistance of Diana Lednitzky and Dr Philip Fletcher at the University of Bath Material and Chemical Characterisation Facility (MC) for SEM and AFM and thank Professor Chris Bowen for access to and use of his profilometer. 2

Funding Information:
This work was funded by New Harvest, a 501(c) (3) non-profit research institute (grant #007) and the EPSRC Centre for Doctoral Training in Sustainable Chemical Technologies (EP/L016354/1). The authors would like to thank the University of Bath for supporting this work, gratefully acknowledge the assistance of Diana Lednitzky and Dr Philip Fletcher at the University of Bath Material and Chemical Characterisation Facility (MC2) for SEM and AFM and thank Professor Chris Bowen for access to and use of his profilometer.

Publisher Copyright:
© 2021 The Authors. Journal of Biomedical Materials Research Part A published by Wiley Periodicals LLC.

ASJC Scopus subject areas

Bioengineering
Biomaterials
Biomedical Engineering

Access to Document

10.1002/jbm.a.37241Licence: CC BY

JBMR-A-20-0369.R2_Proof_hi.PDFAccepted author manuscript, 3 MB
jbm.a.37241Final published version, 5.44 MBLicence: CC BY

Cite this

@article{cf520c1508a84b4cac68cee351025362,

title = "Decellularized grass as a sustainable scaffold for skeletal muscle tissue engineering",

abstract = "Scaffold materials suitable for the scale-up and subsequent commercialization of tissue engineered products should ideally be cost effective and accessible. For the in vitro culture of certain adherent cells, synthetic fabrication techniques are often employed to produce micro- or nano-patterned substrates to influence cell attachment, morphology, and alignment via the mechanism of contact guidance. Here we present a natural scaffold, in the form of decellularized amenity grass, which retains its natural striated topography and supports the attachment, proliferation, alignment and differentiation of murine C2C12 myoblasts, without the need for additional functionalization. This presents an inexpensive, sustainable scaffold material and structure for tissue engineering applications capable of influencing cell alignment, a desired property for the culture of skeletal muscle and other anisotropic tissues.",

author = "Scott Allan and Marianne Ellis and {De Bank}, Paul",

note = "Funding Information: This work was funded by New Harvest, a 501(c) (3) non‐profit research institute (grant #007) and the EPSRC Centre for Doctoral Training in Sustainable Chemical Technologies (EP/L016354/1). The authors would like to thank the University of Bath for supporting this work, gratefully acknowledge the assistance of Diana Lednitzky and Dr Philip Fletcher at the University of Bath Material and Chemical Characterisation Facility (MC) for SEM and AFM and thank Professor Chris Bowen for access to and use of his profilometer. 2 Funding Information: This work was funded by New Harvest, a 501(c) (3) non-profit research institute (grant #007) and the EPSRC Centre for Doctoral Training in Sustainable Chemical Technologies (EP/L016354/1). The authors would like to thank the University of Bath for supporting this work, gratefully acknowledge the assistance of Diana Lednitzky and Dr Philip Fletcher at the University of Bath Material and Chemical Characterisation Facility (MC2) for SEM and AFM and thank Professor Chris Bowen for access to and use of his profilometer. Publisher Copyright: {\textcopyright} 2021 The Authors. Journal of Biomedical Materials Research Part A published by Wiley Periodicals LLC.",

year = "2021",

month = dec,

day = "31",

doi = "10.1002/jbm.a.37241",

language = "English",

volume = "109",

pages = "2471--2482",

journal = "Journal of Biomedical Materials Research - Part A",

issn = "1549-3296",

publisher = "John Wiley & Sons",

number = "12",

}

TY - JOUR

T1 - Decellularized grass as a sustainable scaffold for skeletal muscle tissue engineering

AU - Allan, Scott

AU - Ellis, Marianne

AU - De Bank, Paul

N1 - Funding Information: This work was funded by New Harvest, a 501(c) (3) non‐profit research institute (grant #007) and the EPSRC Centre for Doctoral Training in Sustainable Chemical Technologies (EP/L016354/1). The authors would like to thank the University of Bath for supporting this work, gratefully acknowledge the assistance of Diana Lednitzky and Dr Philip Fletcher at the University of Bath Material and Chemical Characterisation Facility (MC) for SEM and AFM and thank Professor Chris Bowen for access to and use of his profilometer. 2 Funding Information: This work was funded by New Harvest, a 501(c) (3) non-profit research institute (grant #007) and the EPSRC Centre for Doctoral Training in Sustainable Chemical Technologies (EP/L016354/1). The authors would like to thank the University of Bath for supporting this work, gratefully acknowledge the assistance of Diana Lednitzky and Dr Philip Fletcher at the University of Bath Material and Chemical Characterisation Facility (MC2) for SEM and AFM and thank Professor Chris Bowen for access to and use of his profilometer. Publisher Copyright: © 2021 The Authors. Journal of Biomedical Materials Research Part A published by Wiley Periodicals LLC.

PY - 2021/12/31

Y1 - 2021/12/31

N2 - Scaffold materials suitable for the scale-up and subsequent commercialization of tissue engineered products should ideally be cost effective and accessible. For the in vitro culture of certain adherent cells, synthetic fabrication techniques are often employed to produce micro- or nano-patterned substrates to influence cell attachment, morphology, and alignment via the mechanism of contact guidance. Here we present a natural scaffold, in the form of decellularized amenity grass, which retains its natural striated topography and supports the attachment, proliferation, alignment and differentiation of murine C2C12 myoblasts, without the need for additional functionalization. This presents an inexpensive, sustainable scaffold material and structure for tissue engineering applications capable of influencing cell alignment, a desired property for the culture of skeletal muscle and other anisotropic tissues.

AB - Scaffold materials suitable for the scale-up and subsequent commercialization of tissue engineered products should ideally be cost effective and accessible. For the in vitro culture of certain adherent cells, synthetic fabrication techniques are often employed to produce micro- or nano-patterned substrates to influence cell attachment, morphology, and alignment via the mechanism of contact guidance. Here we present a natural scaffold, in the form of decellularized amenity grass, which retains its natural striated topography and supports the attachment, proliferation, alignment and differentiation of murine C2C12 myoblasts, without the need for additional functionalization. This presents an inexpensive, sustainable scaffold material and structure for tissue engineering applications capable of influencing cell alignment, a desired property for the culture of skeletal muscle and other anisotropic tissues.

UR - http://www.scopus.com/inward/record.url?scp=85107021768&partnerID=8YFLogxK

U2 - 10.1002/jbm.a.37241

DO - 10.1002/jbm.a.37241

M3 - Article

SN - 1549-3296

VL - 109

SP - 2471

EP - 2482

JO - Journal of Biomedical Materials Research - Part A

JF - Journal of Biomedical Materials Research - Part A

IS - 12

ER -

Decellularized grass as a sustainable scaffold for skeletal muscle tissue engineering

Abstract

Bibliographical note

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Atomic Force Microscope

MC2-Electron Microscopy (EM)

Cite this

Decellularized grass as a sustainable scaffold for skeletal muscle tissue engineering

Abstract

Bibliographical note

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Equipment

Atomic Force Microscope

MC2-Electron Microscopy (EM)

Cite this