TY - JOUR
T1 - Decellularized grass as a sustainable scaffold for skeletal muscle tissue engineering
AU - Allan, Scott
AU - Ellis, Marianne
AU - De Bank, Paul
N1 - Funding Information:
This work was funded by New Harvest, a 501(c) (3) non‐profit research institute (grant #007) and the EPSRC Centre for Doctoral Training in Sustainable Chemical Technologies (EP/L016354/1). The authors would like to thank the University of Bath for supporting this work, gratefully acknowledge the assistance of Diana Lednitzky and Dr Philip Fletcher at the University of Bath Material and Chemical Characterisation Facility (MC) for SEM and AFM and thank Professor Chris Bowen for access to and use of his profilometer. 2
Funding Information:
This work was funded by New Harvest, a 501(c) (3) non-profit research institute (grant #007) and the EPSRC Centre for Doctoral Training in Sustainable Chemical Technologies (EP/L016354/1). The authors would like to thank the University of Bath for supporting this work, gratefully acknowledge the assistance of Diana Lednitzky and Dr Philip Fletcher at the University of Bath Material and Chemical Characterisation Facility (MC2) for SEM and AFM and thank Professor Chris Bowen for access to and use of his profilometer.
Publisher Copyright:
© 2021 The Authors. Journal of Biomedical Materials Research Part A published by Wiley Periodicals LLC.
PY - 2021/12/31
Y1 - 2021/12/31
N2 - Scaffold materials suitable for the scale-up and subsequent commercialization of tissue engineered products should ideally be cost effective and accessible. For the in vitro culture of certain adherent cells, synthetic fabrication techniques are often employed to produce micro- or nano-patterned substrates to influence cell attachment, morphology, and alignment via the mechanism of contact guidance. Here we present a natural scaffold, in the form of decellularized amenity grass, which retains its natural striated topography and supports the attachment, proliferation, alignment and differentiation of murine C2C12 myoblasts, without the need for additional functionalization. This presents an inexpensive, sustainable scaffold material and structure for tissue engineering applications capable of influencing cell alignment, a desired property for the culture of skeletal muscle and other anisotropic tissues.
AB - Scaffold materials suitable for the scale-up and subsequent commercialization of tissue engineered products should ideally be cost effective and accessible. For the in vitro culture of certain adherent cells, synthetic fabrication techniques are often employed to produce micro- or nano-patterned substrates to influence cell attachment, morphology, and alignment via the mechanism of contact guidance. Here we present a natural scaffold, in the form of decellularized amenity grass, which retains its natural striated topography and supports the attachment, proliferation, alignment and differentiation of murine C2C12 myoblasts, without the need for additional functionalization. This presents an inexpensive, sustainable scaffold material and structure for tissue engineering applications capable of influencing cell alignment, a desired property for the culture of skeletal muscle and other anisotropic tissues.
UR - http://www.scopus.com/inward/record.url?scp=85107021768&partnerID=8YFLogxK
U2 - 10.1002/jbm.a.37241
DO - 10.1002/jbm.a.37241
M3 - Article
SN - 1549-3296
VL - 109
SP - 2471
EP - 2482
JO - Journal of Biomedical Materials Research - Part A
JF - Journal of Biomedical Materials Research - Part A
IS - 12
ER -