De novo green fluorescent protein chromophore-based probes for capturing latent fingerprints using a portable system

Nanan Ruan; Qianfang  Qiu; Xiaoqin Wei; Jiajia Liu; Frank Wu; Nengqin Jia; Chusen Huang; Tony James

doi:10.1021/jacs.3c11277

De novo green fluorescent protein chromophore-based probes for capturing latent fingerprints using a portable system

Nanan Ruan, Qianfang Qiu, Xiaoqin Wei, Jiajia Liu, Frank Wu, Nengqin Jia, Chusen Huang, Tony James

Shanghai Normal University

Research output: Contribution to journal › Article › peer-review

8 Citations (SciVal)

Abstract

Rapid visualization of latent fingerprints, preferably at their point of origin, is essential for effective crime scene evaluation. Here, we present a new class of green fluorescent protein chromophore-based fluorescent dyes (LFP-Yellow and LFP-Red) that can be used for real-time visualization of LFPs within 10 s. Compared with traditional chemical reagents for LFPs, these fluorescent dyes are completely water-soluble, exhibit low cytotoxicity, and are harmless to users. Level 1–3 details of the LFPs could be clearly revealed through “off–on” fluorescence signal readout. Additionally, the fluorescent dyes were constructed based on an imidazolinone core and so do not contain pyridine groups or metal ions, which ensures that the DNA is not contaminated during extraction and identification after the LFPs are treated with the dyes. Combined with our as-developed portable system for capturing LFPs, LFP-Yellow and LFP-Red enabled the rapid capture of LFPs. Therefore, these green fluorescent protein chromophore-based probes provide an approach for the rapid identification of individuals who were present at a crime scene.

Original language	English
Pages (from-to)	2072-2079
Number of pages	8
Journal	Journal of the American Chemical Society
Volume	146
Issue number	3
Early online date	8 Jan 2024
DOIs	https://doi.org/10.1021/jacs.3c11277
Publication status	Published - 24 Jan 2024

Funding

The authors gratefully acknowledge the financial support from the National Natural Science Foundation of China (grants 21672150 and 21302125), Shanghai Rising-Star Program (19QA1406400), and Shanghai Engineering Research Center of Green Energy Chemical Engineering (18DZ2254200). L.W. wishes to thank the University of Bath for supporting his work in the UK. T.D.J. wishes to thank the University of Bath and the Open Research Fund of the School of Chemistry and Chemical Engineering, Henan Normal University (2020ZD01) for support.

Funders	Funder number
Shanghai Engineering Research Center of Green Energy Chemical Engineering	18DZ2254200
Henan Normal University	2020ZD01
University of Bath
National Natural Science Foundation of China	21302125, 21672150
Shanghai Rising-Star Program	19QA1406400

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1021/jacs.3c11277Licence: CC BY

Cite this

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abstract = "Rapid visualization of latent fingerprints, preferably at their point of origin, is essential for effective crime scene evaluation. Here, we present a new class of green fluorescent protein chromophore-based fluorescent dyes (LFP-Yellow and LFP-Red) that can be used for real-time visualization of LFPs within 10 s. Compared with traditional chemical reagents for LFPs, these fluorescent dyes are completely water-soluble, exhibit low cytotoxicity, and are harmless to users. Level 1–3 details of the LFPs could be clearly revealed through “off–on” fluorescence signal readout. Additionally, the fluorescent dyes were constructed based on an imidazolinone core and so do not contain pyridine groups or metal ions, which ensures that the DNA is not contaminated during extraction and identification after the LFPs are treated with the dyes. Combined with our as-developed portable system for capturing LFPs, LFP-Yellow and LFP-Red enabled the rapid capture of LFPs. Therefore, these green fluorescent protein chromophore-based probes provide an approach for the rapid identification of individuals who were present at a crime scene.",

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AU - Liu, Jiajia

AU - Wu, Frank

AU - Jia, Nengqin

AU - Huang, Chusen

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AB - Rapid visualization of latent fingerprints, preferably at their point of origin, is essential for effective crime scene evaluation. Here, we present a new class of green fluorescent protein chromophore-based fluorescent dyes (LFP-Yellow and LFP-Red) that can be used for real-time visualization of LFPs within 10 s. Compared with traditional chemical reagents for LFPs, these fluorescent dyes are completely water-soluble, exhibit low cytotoxicity, and are harmless to users. Level 1–3 details of the LFPs could be clearly revealed through “off–on” fluorescence signal readout. Additionally, the fluorescent dyes were constructed based on an imidazolinone core and so do not contain pyridine groups or metal ions, which ensures that the DNA is not contaminated during extraction and identification after the LFPs are treated with the dyes. Combined with our as-developed portable system for capturing LFPs, LFP-Yellow and LFP-Red enabled the rapid capture of LFPs. Therefore, these green fluorescent protein chromophore-based probes provide an approach for the rapid identification of individuals who were present at a crime scene.

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De novo green fluorescent protein chromophore-based probes for capturing latent fingerprints using a portable system

Abstract

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UN SDGs

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