Cytotoxic effects of tebufenozide in vitro bioassays

Xiaoqin Yu, Yang Zhang, Mingjun Yang, Junfu Guo, Wenping Xu, Jufang Gao, Yaxiao Li, Liming Tao

Research output: Contribution to journalArticlepeer-review

8 Citations (SciVal)

Abstract

Tebufenozide is considered an environmentally friendly pesticide due to its specificity on target insects, but the effects on human are well studied. Studies on the toxicity of tebufenozide at molecular and cellular level is poorly understood. The present study reveals non-selective cytotoxic effects of tebufenozide, and the apoptotic mechanism induced by tebufenozide on HeLa and Tn5B1-4 cells. We demonstrate that the viability of HeLa and Tn5B1-4 cells is inhibited by tebufenozide in a time- and concentration-dependent manner. Intracellular biochemical assays showed that tebufenozide-induced apoptosis of two cell lines concurrent with a decrease in the mitochondrial membrane potential and an increase reactive oxygen species generation, the release of cytochrome-c into the cytosol and a marked activation of caspase-3. These results indicate that a mitochondrial-dependent intrinsic pathway contributes to tebufenozide induced apoptosis in HeLa and Tn5B1-4 cells and suggests potential threats to ecosystems and human health.

Original languageEnglish
Pages (from-to)180-188
Number of pages9
JournalEcotoxicology and Environmental Safety
Volume129
DOIs
Publication statusPublished - 1 Jul 2016

Keywords

  • Apoptosis
  • Cytotoxic effects
  • Environmental toxicity
  • Pesticide
  • Tebufenozide

Fingerprint

Dive into the research topics of 'Cytotoxic effects of tebufenozide in vitro bioassays'. Together they form a unique fingerprint.

Cite this