Cysteine-Cystine Detection in a Gold-Gold Dual-Plate Generator-Collector Microtrench Sensor

Jules L Hammond, A Gross, F Marken, P Estrela

Research output: Contribution to conferencePoster

Abstract

The redox couple cysteine-cystine plays an important physiological role as it defines primary, secondary and tertiary structures of many proteins, and acts as a signaling mechanism through the formation of oxidative post-translational modifications.
Electrochemical detection of the redox couple is traditionally afflicted by complex kinetics and masking from strong adsorption. Here a dual-plate microtrench sensor is operated in generator-collector mode, offering several advantages over conventional electrochemical sensors such as: feedback amplification, discrimination of irreversible interference as well as improved specificity due to the two separately controlled electrode potentials.
Well-defined steady-state voltammetric current responses down to analytically useful micromolar concentrations are achieved in non-degassed aqueous buffer media at pH 7. The currents are found to be kinetically limited and suppressed, possibly by the depletion of analyte in the microtrench due to the anodic over-oxidation of cysteine. The system therefore provides a novel and sensitive sensor of cysteine-cystine with a wide range of applications.

Conference

ConferenceECHEMS 2004 - Electrochemistry in Molecular Understanding
CountryUK United Kingdom
CityWells
Period17/06/1420/06/14

Fingerprint

Cystine
Gold
Cysteine
Sensors
Electrochemical sensors
Amplification
Buffers
Feedback
Adsorption
Oxidation
Electrodes
Kinetics
Proteins
Oxidation-Reduction

Cite this

Hammond, J. L., Gross, A., Marken, F., & Estrela, P. (2014). Cysteine-Cystine Detection in a Gold-Gold Dual-Plate Generator-Collector Microtrench Sensor. Poster session presented at ECHEMS 2004 - Electrochemistry in Molecular Understanding, Wells, UK United Kingdom.

Cysteine-Cystine Detection in a Gold-Gold Dual-Plate Generator-Collector Microtrench Sensor. / Hammond, Jules L; Gross, A; Marken, F; Estrela, P.

2014. Poster session presented at ECHEMS 2004 - Electrochemistry in Molecular Understanding, Wells, UK United Kingdom.

Research output: Contribution to conferencePoster

Hammond, JL, Gross, A, Marken, F & Estrela, P 2014, 'Cysteine-Cystine Detection in a Gold-Gold Dual-Plate Generator-Collector Microtrench Sensor' ECHEMS 2004 - Electrochemistry in Molecular Understanding, Wells, UK United Kingdom, 17/06/14 - 20/06/14, .
Hammond JL, Gross A, Marken F, Estrela P. Cysteine-Cystine Detection in a Gold-Gold Dual-Plate Generator-Collector Microtrench Sensor. 2014. Poster session presented at ECHEMS 2004 - Electrochemistry in Molecular Understanding, Wells, UK United Kingdom.
Hammond, Jules L ; Gross, A ; Marken, F ; Estrela, P. / Cysteine-Cystine Detection in a Gold-Gold Dual-Plate Generator-Collector Microtrench Sensor. Poster session presented at ECHEMS 2004 - Electrochemistry in Molecular Understanding, Wells, UK United Kingdom.
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abstract = "The redox couple cysteine-cystine plays an important physiological role as it defines primary, secondary and tertiary structures of many proteins, and acts as a signaling mechanism through the formation of oxidative post-translational modifications.Electrochemical detection of the redox couple is traditionally afflicted by complex kinetics and masking from strong adsorption. Here a dual-plate microtrench sensor is operated in generator-collector mode, offering several advantages over conventional electrochemical sensors such as: feedback amplification, discrimination of irreversible interference as well as improved specificity due to the two separately controlled electrode potentials.Well-defined steady-state voltammetric current responses down to analytically useful micromolar concentrations are achieved in non-degassed aqueous buffer media at pH 7. The currents are found to be kinetically limited and suppressed, possibly by the depletion of analyte in the microtrench due to the anodic over-oxidation of cysteine. The system therefore provides a novel and sensitive sensor of cysteine-cystine with a wide range of applications.",
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Y1 - 2014

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AB - The redox couple cysteine-cystine plays an important physiological role as it defines primary, secondary and tertiary structures of many proteins, and acts as a signaling mechanism through the formation of oxidative post-translational modifications.Electrochemical detection of the redox couple is traditionally afflicted by complex kinetics and masking from strong adsorption. Here a dual-plate microtrench sensor is operated in generator-collector mode, offering several advantages over conventional electrochemical sensors such as: feedback amplification, discrimination of irreversible interference as well as improved specificity due to the two separately controlled electrode potentials.Well-defined steady-state voltammetric current responses down to analytically useful micromolar concentrations are achieved in non-degassed aqueous buffer media at pH 7. The currents are found to be kinetically limited and suppressed, possibly by the depletion of analyte in the microtrench due to the anodic over-oxidation of cysteine. The system therefore provides a novel and sensitive sensor of cysteine-cystine with a wide range of applications.

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