Abstract
The redox couple cysteine-cystine plays an important physiological role as it defines primary, secondary and tertiary structures of many proteins, and acts as a signaling mechanism through the formation of oxidative post-translational modifications.
Electrochemical detection of the redox couple is traditionally afflicted by complex kinetics and masking from strong adsorption. Here a dual-plate microtrench sensor is operated in generator-collector mode, offering several advantages over conventional electrochemical sensors such as: feedback amplification, discrimination of irreversible interference as well as improved specificity due to the two separately controlled electrode potentials.
Well-defined steady-state voltammetric current responses down to analytically useful micromolar concentrations are achieved in non-degassed aqueous buffer media at pH 7. The currents are found to be kinetically limited and suppressed, possibly by the depletion of analyte in the microtrench due to the anodic over-oxidation of cysteine. The system therefore provides a novel and sensitive sensor of cysteine-cystine with a wide range of applications.
Electrochemical detection of the redox couple is traditionally afflicted by complex kinetics and masking from strong adsorption. Here a dual-plate microtrench sensor is operated in generator-collector mode, offering several advantages over conventional electrochemical sensors such as: feedback amplification, discrimination of irreversible interference as well as improved specificity due to the two separately controlled electrode potentials.
Well-defined steady-state voltammetric current responses down to analytically useful micromolar concentrations are achieved in non-degassed aqueous buffer media at pH 7. The currents are found to be kinetically limited and suppressed, possibly by the depletion of analyte in the microtrench due to the anodic over-oxidation of cysteine. The system therefore provides a novel and sensitive sensor of cysteine-cystine with a wide range of applications.
| Original language | English |
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| Publication status | Unpublished - 2014 |
| Event | ECHEMS 2004 - Electrochemistry in Molecular Understanding - Wells, UK United Kingdom Duration: 17 Jun 2014 → 20 Jun 2014 |
Conference
| Conference | ECHEMS 2004 - Electrochemistry in Molecular Understanding |
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| Country/Territory | UK United Kingdom |
| City | Wells |
| Period | 17/06/14 → 20/06/14 |