Cultivation of an L‐lactate dehydrogenase mutant of Bacillus stearothermophilus in continuous culture with cell recycle

R. San Martin; D. Bushell; D. J. Leak; B. S. Hartley

doi:10.1002/bit.260440105

Cultivation of an L‐lactate dehydrogenase mutant of Bacillus stearothermophilus in continuous culture with cell recycle

R. San Martin
, D. Bushell
, D. J. Leak
, B. S. Hartley

Imperial College London

Research output: Contribution to journal › Article › peer-review

Abstract

Continuous fermentation with cell recycle proved very effective in increasing the ethanol volumetric productivity of the thermophilic facultative anaerobe, Bacillus stearothermophilus strain LLD‐15, on sucrose at 70°C. When complete cell recycle was used, cell viability decreased after a few residence times and sucrose consumption was reduced. Operation using a constant bleed rate resulted in greater stability and higher ethanol volumetric productivities. A mathematical model based on maintenance energy requirements provided an adequate description of the system. © 1994 John Wiley & Sons, Inc.

Original language	English
Pages (from-to)	21-28
Number of pages	8
Journal	Biotechnology and Bioengineering
Volume	44
Issue number	1
DOIs	https://doi.org/10.1002/bit.260440105
Publication status	Published - 1 Jan 1994

Keywords

Bacillus
cell recycle
fermentation
thermophilic ethanol fermentation

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

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10.1002/bit.260440105

Cite this

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title = "Cultivation of an L‐lactate dehydrogenase mutant of Bacillus stearothermophilus in continuous culture with cell recycle",

abstract = "Continuous fermentation with cell recycle proved very effective in increasing the ethanol volumetric productivity of the thermophilic facultative anaerobe, Bacillus stearothermophilus strain LLD‐15, on sucrose at 70°C. When complete cell recycle was used, cell viability decreased after a few residence times and sucrose consumption was reduced. Operation using a constant bleed rate resulted in greater stability and higher ethanol volumetric productivities. A mathematical model based on maintenance energy requirements provided an adequate description of the system. {\textcopyright} 1994 John Wiley \& Sons, Inc.",

keywords = "Bacillus, cell recycle, fermentation, thermophilic ethanol fermentation",

author = "Martin, \{R. San\} and D. Bushell and Leak, \{D. J.\} and Hartley, \{B. S.\}",

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AU - Martin, R. San

AU - Bushell, D.

AU - Leak, D. J.

AU - Hartley, B. S.

PY - 1994/1/1

Y1 - 1994/1/1

N2 - Continuous fermentation with cell recycle proved very effective in increasing the ethanol volumetric productivity of the thermophilic facultative anaerobe, Bacillus stearothermophilus strain LLD‐15, on sucrose at 70°C. When complete cell recycle was used, cell viability decreased after a few residence times and sucrose consumption was reduced. Operation using a constant bleed rate resulted in greater stability and higher ethanol volumetric productivities. A mathematical model based on maintenance energy requirements provided an adequate description of the system. © 1994 John Wiley & Sons, Inc.

AB - Continuous fermentation with cell recycle proved very effective in increasing the ethanol volumetric productivity of the thermophilic facultative anaerobe, Bacillus stearothermophilus strain LLD‐15, on sucrose at 70°C. When complete cell recycle was used, cell viability decreased after a few residence times and sucrose consumption was reduced. Operation using a constant bleed rate resulted in greater stability and higher ethanol volumetric productivities. A mathematical model based on maintenance energy requirements provided an adequate description of the system. © 1994 John Wiley & Sons, Inc.

KW - Bacillus

KW - cell recycle

KW - fermentation

KW - thermophilic ethanol fermentation

UR - https://www.scopus.com/pages/publications/0028765275

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JF - Biotechnology and Bioengineering

IS - 1

ER -

Cultivation of an L‐lactate dehydrogenase mutant of Bacillus stearothermophilus in continuous culture with cell recycle

Abstract

Keywords

ASJC Scopus subject areas

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