Cultivation of an L‐lactate dehydrogenase mutant of Bacillus stearothermophilus in continuous culture with cell recycle

R. San Martin, D. Bushell, D. J. Leak, B. S. Hartley

Research output: Contribution to journalArticle


Continuous fermentation with cell recycle proved very effective in increasing the ethanol volumetric productivity of the thermophilic facultative anaerobe, Bacillus stearothermophilus strain LLD‐15, on sucrose at 70°C. When complete cell recycle was used, cell viability decreased after a few residence times and sucrose consumption was reduced. Operation using a constant bleed rate resulted in greater stability and higher ethanol volumetric productivities. A mathematical model based on maintenance energy requirements provided an adequate description of the system. © 1994 John Wiley & Sons, Inc.

Original languageEnglish
Pages (from-to)21-28
Number of pages8
JournalBiotechnology and Bioengineering
Issue number1
Publication statusPublished - 1 Jan 1994


  • Bacillus
  • cell recycle
  • fermentation
  • thermophilic ethanol fermentation

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this