Cultivation of an L‐lactate dehydrogenase mutant of Bacillus stearothermophilus in continuous culture with cell recycle

R. San Martin, D. Bushell, D. J. Leak, B. S. Hartley

Research output: Contribution to journalArticle

Abstract

Continuous fermentation with cell recycle proved very effective in increasing the ethanol volumetric productivity of the thermophilic facultative anaerobe, Bacillus stearothermophilus strain LLD‐15, on sucrose at 70°C. When complete cell recycle was used, cell viability decreased after a few residence times and sucrose consumption was reduced. Operation using a constant bleed rate resulted in greater stability and higher ethanol volumetric productivities. A mathematical model based on maintenance energy requirements provided an adequate description of the system. © 1994 John Wiley & Sons, Inc.

Original languageEnglish
Pages (from-to)21-28
Number of pages8
JournalBiotechnology and Bioengineering
Volume44
Issue number1
DOIs
Publication statusPublished - 1 Jan 1994

Keywords

  • Bacillus
  • cell recycle
  • fermentation
  • thermophilic ethanol fermentation

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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