Crystallization and preliminary X-ray analysis of a microbial superantigen staphylococcal enterotoxin C2

Edward F. Passalacqua; Rossalyn D. Brehm; K. Ravi Acharya; Howard S. Tranter

doi:10.1006/jmbi.1993.1493

Crystallization and preliminary X-ray analysis of a microbial superantigen staphylococcal enterotoxin C2

Edward F. Passalacqua, Rossalyn D. Brehm, K. Ravi Acharya, Howard S. Tranter

Department of Life Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

High yields of staphylococcal enterotoxin C2, from Staphylococcus aureus, have been purified using dye ligand chromatography. Crystals suitable for X-ray diffraction work were obtained by vapour diffusion using ammonium sulphate and polyethylene glycol as precipitants. They belong to the tetragonal space group P4₁₂₂ with unit cell dimensions a=b=43.2 Å and c=290.9 Å with one molecule per asymmetric unit. The crystals diffract to at least 2.8 Å resolution and arc suitable for three-dimensional X-ray structural analysis.

Original language	English
Pages (from-to)	170-172
Number of pages	3
Journal	Journal of Molecular Biology
Volume	233
Issue number	1
DOIs	https://doi.org/10.1006/jmbi.1993.1493
Publication status	Published - 1 Jan 1993

Keywords

Crystallization
Staphylococcal enterotoxin
Superantigen
X-ray diffraction

ASJC Scopus subject areas

Molecular Biology

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10.1006/jmbi.1993.1493

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abstract = "High yields of staphylococcal enterotoxin C2, from Staphylococcus aureus, have been purified using dye ligand chromatography. Crystals suitable for X-ray diffraction work were obtained by vapour diffusion using ammonium sulphate and polyethylene glycol as precipitants. They belong to the tetragonal space group P4122 with unit cell dimensions a=b=43.2 {\AA} and c=290.9 {\AA} with one molecule per asymmetric unit. The crystals diffract to at least 2.8 {\AA} resolution and arc suitable for three-dimensional X-ray structural analysis.",

keywords = "Crystallization, Staphylococcal enterotoxin, Superantigen, X-ray diffraction",

author = "Passalacqua, {Edward F.} and Brehm, {Rossalyn D.} and Acharya, {K. Ravi} and Tranter, {Howard S.}",

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AU - Passalacqua, Edward F.

AU - Brehm, Rossalyn D.

AU - Acharya, K. Ravi

AU - Tranter, Howard S.

PY - 1993/1/1

Y1 - 1993/1/1

N2 - High yields of staphylococcal enterotoxin C2, from Staphylococcus aureus, have been purified using dye ligand chromatography. Crystals suitable for X-ray diffraction work were obtained by vapour diffusion using ammonium sulphate and polyethylene glycol as precipitants. They belong to the tetragonal space group P4122 with unit cell dimensions a=b=43.2 Å and c=290.9 Å with one molecule per asymmetric unit. The crystals diffract to at least 2.8 Å resolution and arc suitable for three-dimensional X-ray structural analysis.

AB - High yields of staphylococcal enterotoxin C2, from Staphylococcus aureus, have been purified using dye ligand chromatography. Crystals suitable for X-ray diffraction work were obtained by vapour diffusion using ammonium sulphate and polyethylene glycol as precipitants. They belong to the tetragonal space group P4122 with unit cell dimensions a=b=43.2 Å and c=290.9 Å with one molecule per asymmetric unit. The crystals diffract to at least 2.8 Å resolution and arc suitable for three-dimensional X-ray structural analysis.

KW - Crystallization

KW - Staphylococcal enterotoxin

KW - Superantigen

KW - X-ray diffraction

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ER -

Crystallization and preliminary X-ray analysis of a microbial superantigen staphylococcal enterotoxin C2

Abstract

Keywords

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