Crystallization and preliminary X-ray analysis of a microbial superantigen staphylococcal enterotoxin C2

Edward F. Passalacqua, Rossalyn D. Brehm, K. Ravi Acharya, Howard S. Tranter

Research output: Contribution to journalArticle

Abstract

High yields of staphylococcal enterotoxin C2, from Staphylococcus aureus, have been purified using dye ligand chromatography. Crystals suitable for X-ray diffraction work were obtained by vapour diffusion using ammonium sulphate and polyethylene glycol as precipitants. They belong to the tetragonal space group P4122 with unit cell dimensions a=b=43.2 Å and c=290.9 Å with one molecule per asymmetric unit. The crystals diffract to at least 2.8 Å resolution and arc suitable for three-dimensional X-ray structural analysis.

Original languageEnglish
Pages (from-to)170-172
Number of pages3
JournalJournal of Molecular Biology
Volume233
Issue number1
DOIs
Publication statusPublished - 1 Jan 1993

Keywords

  • Crystallization
  • Staphylococcal enterotoxin
  • Superantigen
  • X-ray diffraction

ASJC Scopus subject areas

  • Molecular Biology

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