Crystal structure of NucB, a biofilm-degrading endonuclease

Arnaud Baslé, Lorraine Hewitt, Alan Koh, Heather K Lamb, Paul Thompson, J Grant Burgess, Michael J Hall, Alastair R Hawkins, Heath Murray, Richard J Lewis

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Bacterial biofilms are a complex architecture of cells that grow on moist interfaces, and are held together by a molecular glue of extracellular proteins, sugars and nucleic acids. Biofilms are particularly problematic in human healthcare as they can coat medical implants and are thus a potential source of disease. The enzymatic dispersal of biofilms is increasingly being developed as a new strategy to treat this problem. Here, we have characterized NucB, a biofilm-dispersing nuclease from a marine strain of Bacillus licheniformis, and present its crystal structure together with the biochemistry and a mutational analysis required to confirm its active site. Taken together, these data support the categorization of NucB into a unique subfamily of the metal-dependent nonspecific endonucleases. Understanding the structure and function of NucB will facilitate its future development into an anti-biofilm therapeutic agent.

Original languageEnglish
Pages (from-to)473-484
Number of pages12
JournalNucleic Acids Research
Volume46
Issue number1
Early online date20 Nov 2017
DOIs
Publication statusPublished - 9 Jan 2018

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Endonucleases
Biofilms
Sugar Acids
Adhesives
Biochemistry
Nucleic Acids
Catalytic Domain
Metals
Delivery of Health Care
Proteins

ASJC Scopus subject areas

  • Genetics

Cite this

Baslé, A., Hewitt, L., Koh, A., Lamb, H. K., Thompson, P., Burgess, J. G., ... Lewis, R. J. (2018). Crystal structure of NucB, a biofilm-degrading endonuclease. Nucleic Acids Research, 46(1), 473-484. https://doi.org/10.1093/nar/gkx1170

Crystal structure of NucB, a biofilm-degrading endonuclease. / Baslé, Arnaud; Hewitt, Lorraine; Koh, Alan; Lamb, Heather K; Thompson, Paul; Burgess, J Grant; Hall, Michael J; Hawkins, Alastair R; Murray, Heath; Lewis, Richard J.

In: Nucleic Acids Research, Vol. 46, No. 1, 09.01.2018, p. 473-484.

Research output: Contribution to journalArticle

Baslé, A, Hewitt, L, Koh, A, Lamb, HK, Thompson, P, Burgess, JG, Hall, MJ, Hawkins, AR, Murray, H & Lewis, RJ 2018, 'Crystal structure of NucB, a biofilm-degrading endonuclease', Nucleic Acids Research, vol. 46, no. 1, pp. 473-484. https://doi.org/10.1093/nar/gkx1170
Baslé A, Hewitt L, Koh A, Lamb HK, Thompson P, Burgess JG et al. Crystal structure of NucB, a biofilm-degrading endonuclease. Nucleic Acids Research. 2018 Jan 9;46(1):473-484. https://doi.org/10.1093/nar/gkx1170
Baslé, Arnaud ; Hewitt, Lorraine ; Koh, Alan ; Lamb, Heather K ; Thompson, Paul ; Burgess, J Grant ; Hall, Michael J ; Hawkins, Alastair R ; Murray, Heath ; Lewis, Richard J. / Crystal structure of NucB, a biofilm-degrading endonuclease. In: Nucleic Acids Research. 2018 ; Vol. 46, No. 1. pp. 473-484.
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