Crystal structure of NucB, a biofilm-degrading endonuclease

Arnaud Baslé, Lorraine Hewitt, Alan Koh, Heather K Lamb, Paul Thompson, J Grant Burgess, Michael J Hall, Alastair R Hawkins, Heath Murray, Richard J Lewis

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Bacterial biofilms are a complex architecture of cells that grow on moist interfaces, and are held together by a molecular glue of extracellular proteins, sugars and nucleic acids. Biofilms are particularly problematic in human healthcare as they can coat medical implants and are thus a potential source of disease. The enzymatic dispersal of biofilms is increasingly being developed as a new strategy to treat this problem. Here, we have characterized NucB, a biofilm-dispersing nuclease from a marine strain of Bacillus licheniformis, and present its crystal structure together with the biochemistry and a mutational analysis required to confirm its active site. Taken together, these data support the categorization of NucB into a unique subfamily of the metal-dependent nonspecific endonucleases. Understanding the structure and function of NucB will facilitate its future development into an anti-biofilm therapeutic agent.

Original languageEnglish
Pages (from-to)473-484
Number of pages12
JournalNucleic Acids Research
Volume46
Issue number1
Early online date20 Nov 2017
DOIs
Publication statusPublished - 9 Jan 2018

ASJC Scopus subject areas

  • Genetics

Fingerprint Dive into the research topics of 'Crystal structure of NucB, a biofilm-degrading endonuclease'. Together they form a unique fingerprint.

  • Cite this

    Baslé, A., Hewitt, L., Koh, A., Lamb, H. K., Thompson, P., Burgess, J. G., Hall, M. J., Hawkins, A. R., Murray, H., & Lewis, R. J. (2018). Crystal structure of NucB, a biofilm-degrading endonuclease. Nucleic Acids Research, 46(1), 473-484. https://doi.org/10.1093/nar/gkx1170