Crystal structure of eosinophil cationic protein at 2.4 A resolution

Ester Boix, Demetres D. Leonidas, Zoran Nikolovski, M. Victòria Nogués, Claudi M. Cuchillo, K. Ravi Acharya

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Abstract

Eosinophil cationic protein (ECP) is located in the matrix of the eosinophil's large specific granule and has marked toxicity for a variety of helminth parasites, hemoflagellates, bacteria, single-stranded RNA virus, and mammalian cells and tissues. It belongs to the bovine pancreatic ribonuclease A (RNase A) family and exhibits ribonucleolytic activity which is about 100- fold lower than that of a related eosinophil ribonuclease, the eosinophil- derived neurotoxin (EDN). The crystal structure of human ECP, determined at 2.4 A, is similar to that of RNase A and EDN. It reveals that residues Gln- 14, His-15, Lys-38, Thr-42, and His-128 at the active site are conserved as in all other RNase A homologues. Nevertheless, evidence for considerable divergence of ECP is also implicit in the structure. Amino acid residues Arg- 7, Trp-10, Asn-39, His-64, and His-82 appear to play a key part in the Substrate specificity and low catalytic activity of ECP. The structure also shows how the cationic residues are distributed on the surface of the ECP molecule that may have implications for an understanding of the cytotoxicity of this enzyme.

Original languageEnglish
Pages (from-to)16794-16801
Number of pages8
JournalBiochemistry
Volume38
Issue number51
DOIs
Publication statusPublished - 21 Dec 1999

ASJC Scopus subject areas

  • Biochemistry

Cite this

Boix, E., Leonidas, D. D., Nikolovski, Z., Nogués, M. V., Cuchillo, C. M., & Acharya, K. R. (1999). Crystal structure of eosinophil cationic protein at 2.4 A resolution. Biochemistry, 38(51), 16794-16801. https://doi.org/10.1021/bi9919145

Crystal structure of eosinophil cationic protein at 2.4 A resolution. / Boix, Ester; Leonidas, Demetres D.; Nikolovski, Zoran; Nogués, M. Victòria; Cuchillo, Claudi M.; Acharya, K. Ravi.

In: Biochemistry, Vol. 38, No. 51, 21.12.1999, p. 16794-16801.

Research output: Contribution to journalArticle

Boix, E, Leonidas, DD, Nikolovski, Z, Nogués, MV, Cuchillo, CM & Acharya, KR 1999, 'Crystal structure of eosinophil cationic protein at 2.4 A resolution', Biochemistry, vol. 38, no. 51, pp. 16794-16801. https://doi.org/10.1021/bi9919145
Boix E, Leonidas DD, Nikolovski Z, Nogués MV, Cuchillo CM, Acharya KR. Crystal structure of eosinophil cationic protein at 2.4 A resolution. Biochemistry. 1999 Dec 21;38(51):16794-16801. https://doi.org/10.1021/bi9919145
Boix, Ester ; Leonidas, Demetres D. ; Nikolovski, Zoran ; Nogués, M. Victòria ; Cuchillo, Claudi M. ; Acharya, K. Ravi. / Crystal structure of eosinophil cationic protein at 2.4 A resolution. In: Biochemistry. 1999 ; Vol. 38, No. 51. pp. 16794-16801.
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