TY - JOUR
T1 - Cross-reactive sensor arrays for the detection of peptides in aqueous solution by fluorescence spectroscopy
AU - Rochat, Sébastien
AU - Gao, Jie
AU - Qian, Xuhong
AU - Zaubitzer, Friederike
AU - Severin, Kay
PY - 2010/1/4
Y1 - 2010/1/4
N2 - A simple but powerful method for the sensing of peptides in aqueous solution has been developed. The transition-metal complexes [PdCl 2(en)], [{RhCl2Cp*}2], and [{RuCl 2(p-cymene)}2] were combined with six different fluorescent dyes to build a cross-reactive sensor array. The fluorescence response of the individual sensor units was based on competitive complexation reactions between the peptide analytes and the fluorescent dyes. The collective response of the sensor array in a time-resolved fashion was used as an input for multivariate analyses. A sensor array comprised of only six metal-dye combinations was able to differentiate ten different dipeptides in buffered aqueous solution at a concentration of 50 μM. Further more, the cross-reactive sensor could be used to obtain information about the identity and the quantity of the pharmacologically interesting dipeptides carnosine and homocarnosine in a complex biological matrix, such as deproteinized human blood serum. The sensor array was also able to sense longer peptides, which was demonstrated by differentiating mixtures of the nonapeptide bradykinin and the decapeptide kallidin.
AB - A simple but powerful method for the sensing of peptides in aqueous solution has been developed. The transition-metal complexes [PdCl 2(en)], [{RhCl2Cp*}2], and [{RuCl 2(p-cymene)}2] were combined with six different fluorescent dyes to build a cross-reactive sensor array. The fluorescence response of the individual sensor units was based on competitive complexation reactions between the peptide analytes and the fluorescent dyes. The collective response of the sensor array in a time-resolved fashion was used as an input for multivariate analyses. A sensor array comprised of only six metal-dye combinations was able to differentiate ten different dipeptides in buffered aqueous solution at a concentration of 50 μM. Further more, the cross-reactive sensor could be used to obtain information about the identity and the quantity of the pharmacologically interesting dipeptides carnosine and homocarnosine in a complex biological matrix, such as deproteinized human blood serum. The sensor array was also able to sense longer peptides, which was demonstrated by differentiating mixtures of the nonapeptide bradykinin and the decapeptide kallidin.
KW - Fluorescence spectroscopy
KW - Indicator displacement assay
KW - Organometallic complexes
KW - Peptides
KW - Sensors
UR - http://www.scopus.com/inward/record.url?scp=73949099245&partnerID=8YFLogxK
UR - http://dx.doi.org/10.1002/chem.200902202
U2 - 10.1002/chem.200902202
DO - 10.1002/chem.200902202
M3 - Article
C2 - 19938007
AN - SCOPUS:73949099245
SN - 0947-6539
VL - 16
SP - 104
EP - 113
JO - Chemistry - A European Journal
JF - Chemistry - A European Journal
IS - 1
ER -