CRISPR-based electrochemical sensor for COVID-19 diagnostics

Josh Rainbow; Helena De Puig; Pawan Jolly; Sanjay Timilsina; Pedro Estrela; James Collins; Donald E. Ingber

CRISPR-based electrochemical sensor for COVID-19 diagnostics

Josh Rainbow
, Helena De Puig
, Pawan Jolly
, Sanjay Timilsina
, Pedro Estrela
, James Collins
, Donald E. Ingber

Harvard University
Massachusetts Institute of Technology
Broad Institute of MIT and Harvard
Harvard-Massachusetts Institute of Technology Program in Health Sciences and Technology
Boston Children’s Hospital

Research output: Chapter or section in a book/report/conference proceeding › Chapter in a published conference proceeding

Abstract

We report the development of an electrochemical sensor platform for ultrasensitive and rapid detection of SARS-CoV-2 viral RNA that integrates loop-mediated isothermal amplification (LAMP), CRISPR-based detection, and anti-fouling nanocomposite coating. By integrating LAMP amplification with CRISPR, we achieved ultrasensitive detection of SARS-CoV-2 RNA at levels as low as 5 copies µL-1. Data from this electrochemical diagnostic platform was comparable to traditional RT-PCR methodology in a fraction of the time, at low cost, and without requiring laboratory space.

Original language	English
Title of host publication	Proceedings of MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Publisher	Chemical and Biological Microsystems Society
Pages	1441-1442
Number of pages	2
ISBN (Electronic)	978-173341903-1
Publication status	Published - 10 Oct 2021
Event	MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences - Duration: 10 Oct 2021 → 14 Oct 2021

Conference

Conference	MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Period	10/10/21 → 14/10/21

Cite this

CRISPR-based electrochemical sensor for COVID-19 diagnostics. / Rainbow, Josh; De Puig, Helena; Jolly, Pawan et al.
Proceedings of MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, 2021. p. 1441-1442.

Research output: Chapter or section in a book/report/conference proceeding › Chapter in a published conference proceeding

Rainbow, J, De Puig, H, Jolly, P, Timilsina, S, Estrela, P, Collins, J & Ingber, DE 2021, CRISPR-based electrochemical sensor for COVID-19 diagnostics. in Proceedings of MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, pp. 1441-1442, MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, 10/10/21.

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title = "CRISPR-based electrochemical sensor for COVID-19 diagnostics",

abstract = "We report the development of an electrochemical sensor platform for ultrasensitive and rapid detection of SARS-CoV-2 viral RNA that integrates loop-mediated isothermal amplification (LAMP), CRISPR-based detection, and anti-fouling nanocomposite coating. By integrating LAMP amplification with CRISPR, we achieved ultrasensitive detection of SARS-CoV-2 RNA at levels as low as 5 copies µL-1. Data from this electrochemical diagnostic platform was comparable to traditional RT-PCR methodology in a fraction of the time, at low cost, and without requiring laboratory space. ",

author = "Josh Rainbow and \{De Puig\}, Helena and Pawan Jolly and Sanjay Timilsina and Pedro Estrela and James Collins and Ingber, \{Donald E.\}",

year = "2021",

month = oct,

day = "10",

language = "English",

pages = "1441--1442",

booktitle = "Proceedings of MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences",

publisher = "Chemical and Biological Microsystems Society",

note = "MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences ; Conference date: 10-10-2021 Through 14-10-2021",

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TY - GEN

T1 - CRISPR-based electrochemical sensor for COVID-19 diagnostics

AU - Rainbow, Josh

AU - De Puig, Helena

AU - Jolly, Pawan

AU - Timilsina, Sanjay

AU - Estrela, Pedro

AU - Collins, James

AU - Ingber, Donald E.

PY - 2021/10/10

Y1 - 2021/10/10

N2 - We report the development of an electrochemical sensor platform for ultrasensitive and rapid detection of SARS-CoV-2 viral RNA that integrates loop-mediated isothermal amplification (LAMP), CRISPR-based detection, and anti-fouling nanocomposite coating. By integrating LAMP amplification with CRISPR, we achieved ultrasensitive detection of SARS-CoV-2 RNA at levels as low as 5 copies µL-1. Data from this electrochemical diagnostic platform was comparable to traditional RT-PCR methodology in a fraction of the time, at low cost, and without requiring laboratory space.

AB - We report the development of an electrochemical sensor platform for ultrasensitive and rapid detection of SARS-CoV-2 viral RNA that integrates loop-mediated isothermal amplification (LAMP), CRISPR-based detection, and anti-fouling nanocomposite coating. By integrating LAMP amplification with CRISPR, we achieved ultrasensitive detection of SARS-CoV-2 RNA at levels as low as 5 copies µL-1. Data from this electrochemical diagnostic platform was comparable to traditional RT-PCR methodology in a fraction of the time, at low cost, and without requiring laboratory space.

UR - https://www.scopus.com/pages/publications/85136992853

M3 - Chapter in a published conference proceeding

SP - 1441

EP - 1442

BT - Proceedings of MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences

PB - Chemical and Biological Microsystems Society

T2 - MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Y2 - 10 October 2021 through 14 October 2021

ER -

CRISPR-based electrochemical sensor for COVID-19 diagnostics

Abstract

Conference

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