Abstract
We report the development of an electrochemical sensor platform for ultrasensitive and rapid detection of SARS-CoV-2 viral RNA that integrates loop-mediated isothermal amplification (LAMP), CRISPR-based detection, and anti-fouling nanocomposite coating. By integrating LAMP amplification with CRISPR, we achieved ultrasensitive detection of SARS-CoV-2 RNA at levels as low as 5 copies µL-1. Data from this electrochemical diagnostic platform was comparable to traditional RT-PCR methodology in a fraction of the time, at low cost, and without requiring laboratory space.
Original language | English |
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Title of host publication | Proceedings of MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences |
Publisher | Chemical and Biological Microsystems Society |
Pages | 1441-1442 |
Number of pages | 2 |
ISBN (Electronic) | 978-173341903-1 |
Publication status | Published - 10 Oct 2021 |
Event | MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences - Duration: 10 Oct 2021 → 14 Oct 2021 |
Conference
Conference | MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences |
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Period | 10/10/21 → 14/10/21 |