Abstract

We report the development of an electrochemical sensor platform for ultrasensitive and rapid detection of SARS-CoV-2 viral RNA that integrates loop-mediated isothermal amplification (LAMP), CRISPR-based detection, and anti-fouling nanocomposite coating. By integrating LAMP amplification with CRISPR, we achieved ultrasensitive detection of SARS-CoV-2 RNA at levels as low as 5 copies µL-1. Data from this electrochemical diagnostic platform was comparable to traditional RT-PCR methodology in a fraction of the time, at low cost, and without requiring laboratory space.
Original languageEnglish
Title of host publicationProceedings of MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherChemical and Biological Microsystems Society
Pages1441-1442
Number of pages2
ISBN (Electronic)978-173341903-1
Publication statusPublished - 10 Oct 2021
EventMicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences -
Duration: 10 Oct 202114 Oct 2021

Conference

ConferenceMicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Period10/10/2114/10/21

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