Countershading in zebrafish results from an Asip1 controlled dorsoventral gradient of pigment cell differentiation.

Laura Cal, Paula Suarez-Bregua, Pilar Comesana, Jennifer Owen, Ingo Braasch, Robert N. Kelsh, Jose Cerda-Reverter, Josep Rotllant

Research output: Contribution to journalArticle

4 Citations (Scopus)
8 Downloads (Pure)

Abstract

Dorso-ventral (DV) countershading is a highly-conserved pigmentary adaptation in vertebrates. In mammals, spatially regulated expression of agouti-signaling protein (ASIP) generates the difference in shading by driving a switch between the production of chemically-distinct melanins in melanocytes in dorsal and ventral regions. In contrast, fish countershading seemed to result from a patterned DV distribution of differently-coloured cell-types (chromatophores). Despite the cellular differences in the basis for counter-shading, previous observations suggested that Agouti signaling likely played a role in this patterning process in fish. To test the hypotheses that Agouti regulated counter-shading in fish, and that this depended upon spatial regulation of the numbers of each chromatophore type, we engineered asip1 homozygous knockout mutant zebrafish. We show that loss-of-function asip1 mutants lose DV countershading, and that this results from changed numbers of multiple pigment cell-types in the skin and on scales. Our findings identify asip1 as key in the establishment of DV countershading in fish, but show that the cellular mechanism for translating a conserved signaling gradient into a conserved pigmentary phenotype has been radically altered in the course of evolution.
Original languageEnglish
Article number3449
Pages (from-to)1-13
Number of pages13
JournalScientific Reports
Volume9
Issue number1
Early online date5 Mar 2019
DOIs
Publication statusE-pub ahead of print - 5 Mar 2019

ASJC Scopus subject areas

  • General

Cite this

Countershading in zebrafish results from an Asip1 controlled dorsoventral gradient of pigment cell differentiation. / Cal, Laura; Suarez-Bregua, Paula; Comesana, Pilar; Owen, Jennifer; Braasch, Ingo; Kelsh, Robert N.; Cerda-Reverter, Jose; Rotllant, Josep.

In: Scientific Reports, Vol. 9, No. 1, 3449, 01.12.2019, p. 1-13.

Research output: Contribution to journalArticle

Cal, Laura ; Suarez-Bregua, Paula ; Comesana, Pilar ; Owen, Jennifer ; Braasch, Ingo ; Kelsh, Robert N. ; Cerda-Reverter, Jose ; Rotllant, Josep. / Countershading in zebrafish results from an Asip1 controlled dorsoventral gradient of pigment cell differentiation. In: Scientific Reports. 2019 ; Vol. 9, No. 1. pp. 1-13.
@article{0cfbcf408a2a4cc5b6cf9225b2c6f6d4,
title = "Countershading in zebrafish results from an Asip1 controlled dorsoventral gradient of pigment cell differentiation.",
abstract = "Dorso-ventral (DV) countershading is a highly-conserved pigmentary adaptation in vertebrates. In mammals, spatially regulated expression of agouti-signaling protein (ASIP) generates the difference in shading by driving a switch between the production of chemically-distinct melanins in melanocytes in dorsal and ventral regions. In contrast, fish countershading seemed to result from a patterned DV distribution of differently-coloured cell-types (chromatophores). Despite the cellular differences in the basis for counter-shading, previous observations suggested that Agouti signaling likely played a role in this patterning process in fish. To test the hypotheses that Agouti regulated counter-shading in fish, and that this depended upon spatial regulation of the numbers of each chromatophore type, we engineered asip1 homozygous knockout mutant zebrafish. We show that loss-of-function asip1 mutants lose DV countershading, and that this results from changed numbers of multiple pigment cell-types in the skin and on scales. Our findings identify asip1 as key in the establishment of DV countershading in fish, but show that the cellular mechanism for translating a conserved signaling gradient into a conserved pigmentary phenotype has been radically altered in the course of evolution.",
author = "Laura Cal and Paula Suarez-Bregua and Pilar Comesana and Jennifer Owen and Ingo Braasch and Kelsh, {Robert N.} and Jose Cerda-Reverter and Josep Rotllant",
year = "2019",
month = "3",
day = "5",
doi = "10.1038/s41598-019-40251-z",
language = "English",
volume = "9",
pages = "1--13",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",
number = "1",

}

TY - JOUR

T1 - Countershading in zebrafish results from an Asip1 controlled dorsoventral gradient of pigment cell differentiation.

AU - Cal, Laura

AU - Suarez-Bregua, Paula

AU - Comesana, Pilar

AU - Owen, Jennifer

AU - Braasch, Ingo

AU - Kelsh, Robert N.

AU - Cerda-Reverter, Jose

AU - Rotllant, Josep

PY - 2019/3/5

Y1 - 2019/3/5

N2 - Dorso-ventral (DV) countershading is a highly-conserved pigmentary adaptation in vertebrates. In mammals, spatially regulated expression of agouti-signaling protein (ASIP) generates the difference in shading by driving a switch between the production of chemically-distinct melanins in melanocytes in dorsal and ventral regions. In contrast, fish countershading seemed to result from a patterned DV distribution of differently-coloured cell-types (chromatophores). Despite the cellular differences in the basis for counter-shading, previous observations suggested that Agouti signaling likely played a role in this patterning process in fish. To test the hypotheses that Agouti regulated counter-shading in fish, and that this depended upon spatial regulation of the numbers of each chromatophore type, we engineered asip1 homozygous knockout mutant zebrafish. We show that loss-of-function asip1 mutants lose DV countershading, and that this results from changed numbers of multiple pigment cell-types in the skin and on scales. Our findings identify asip1 as key in the establishment of DV countershading in fish, but show that the cellular mechanism for translating a conserved signaling gradient into a conserved pigmentary phenotype has been radically altered in the course of evolution.

AB - Dorso-ventral (DV) countershading is a highly-conserved pigmentary adaptation in vertebrates. In mammals, spatially regulated expression of agouti-signaling protein (ASIP) generates the difference in shading by driving a switch between the production of chemically-distinct melanins in melanocytes in dorsal and ventral regions. In contrast, fish countershading seemed to result from a patterned DV distribution of differently-coloured cell-types (chromatophores). Despite the cellular differences in the basis for counter-shading, previous observations suggested that Agouti signaling likely played a role in this patterning process in fish. To test the hypotheses that Agouti regulated counter-shading in fish, and that this depended upon spatial regulation of the numbers of each chromatophore type, we engineered asip1 homozygous knockout mutant zebrafish. We show that loss-of-function asip1 mutants lose DV countershading, and that this results from changed numbers of multiple pigment cell-types in the skin and on scales. Our findings identify asip1 as key in the establishment of DV countershading in fish, but show that the cellular mechanism for translating a conserved signaling gradient into a conserved pigmentary phenotype has been radically altered in the course of evolution.

UR - http://www.scopus.com/inward/record.url?scp=85062585531&partnerID=8YFLogxK

U2 - 10.1038/s41598-019-40251-z

DO - 10.1038/s41598-019-40251-z

M3 - Article

VL - 9

SP - 1

EP - 13

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

IS - 1

M1 - 3449

ER -