Coumarin-based fluorescent probe for the detection of glutathione and nitroreductase

Xue Tian; Lokesh K. Kumawat; Steven D. Bull; Robert B. P. Elmes; Luling Wu; Tony D. James

doi:10.1016/j.tet.2020.131890

Coumarin-based fluorescent probe for the detection of glutathione and nitroreductase

Xue Tian, Lokesh K. Kumawat, Steven D. Bull, Robert B. P. Elmes, Luling Wu, Tony D. James

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Abstract

With this research we set out to develop a coumarin-based novel fluorescent probe NTR-AHC for the detection of biological thiols and nitroreductase (NTR). Probe NTR-AHC was constructed by attaching the NTR trigger moiety (p-nitrobenzyl) and biothiol acceptor (maleic acid monoamide), to the core fluorophore AHC. In the presence of both glutathione (GSH) and NTR, probe NTR-AHC produced a >8-fold increase in fluorescence intensity at 463 nm.

Original language	English
Article number	131890
Journal	Tetrahedron
Volume	82
Early online date	28 Dec 2020
DOIs	https://doi.org/10.1016/j.tet.2020.131890
Publication status	Published - 26 Feb 2021

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10.1016/j.tet.2020.131890

Tetrahedron manuscript 24012021.PDFAccepted author manuscript, 557 KBLicence: CC BY-NC-ND

https://linkinghub.elsevier.com/retrieve/pii/S0040402020311546

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title = "Coumarin-based fluorescent probe for the detection of glutathione and nitroreductase",

abstract = "With this research we set out to develop a coumarin-based novel fluorescent probe NTR-AHC for the detection of biological thiols and nitroreductase (NTR). Probe NTR-AHC was constructed by attaching the NTR trigger moiety (p-nitrobenzyl) and biothiol acceptor (maleic acid monoamide), to the core fluorophore AHC. In the presence of both glutathione (GSH) and NTR, probe NTR-AHC produced a >8-fold increase in fluorescence intensity at 463 nm.",

author = "Xue Tian and Kumawat, {Lokesh K.} and Bull, {Steven D.} and Elmes, {Robert B. P.} and Luling Wu and James, {Tony D.}",

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T1 - Coumarin-based fluorescent probe for the detection of glutathione and nitroreductase

AU - Tian, Xue

AU - Kumawat, Lokesh K.

AU - Bull, Steven D.

AU - Elmes, Robert B. P.

AU - Wu, Luling

AU - James, Tony D.

PY - 2021/2/26

Y1 - 2021/2/26

N2 - With this research we set out to develop a coumarin-based novel fluorescent probe NTR-AHC for the detection of biological thiols and nitroreductase (NTR). Probe NTR-AHC was constructed by attaching the NTR trigger moiety (p-nitrobenzyl) and biothiol acceptor (maleic acid monoamide), to the core fluorophore AHC. In the presence of both glutathione (GSH) and NTR, probe NTR-AHC produced a >8-fold increase in fluorescence intensity at 463 nm.

AB - With this research we set out to develop a coumarin-based novel fluorescent probe NTR-AHC for the detection of biological thiols and nitroreductase (NTR). Probe NTR-AHC was constructed by attaching the NTR trigger moiety (p-nitrobenzyl) and biothiol acceptor (maleic acid monoamide), to the core fluorophore AHC. In the presence of both glutathione (GSH) and NTR, probe NTR-AHC produced a >8-fold increase in fluorescence intensity at 463 nm.

U2 - 10.1016/j.tet.2020.131890

DO - 10.1016/j.tet.2020.131890

M3 - Article

VL - 82

JO - Tetrahedron

JF - Tetrahedron

SN - 0040-4020

M1 - 131890

ER -

Coumarin-based fluorescent probe for the detection of glutathione and nitroreductase

Abstract

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