Coumarin-based fluorescent probe for the detection of glutathione and nitroreductase

Xue Tian, Lokesh K. Kumawat, Steven D. Bull, Robert B. P. Elmes, Luling Wu, Tony D. James

Research output: Contribution to journalArticlepeer-review

Abstract

With this research we set out to develop a coumarin-based novel fluorescent probe NTR-AHC for the detection of biological thiols and nitroreductase (NTR). Probe NTR-AHC was constructed by attaching the NTR trigger moiety (p-nitrobenzyl) and biothiol acceptor (maleic acid monoamide), to the core fluorophore AHC. In the presence of both glutathione (GSH) and NTR, probe NTR-AHC produced a >8-fold increase in fluorescence intensity at 463 nm.
Original languageEnglish
Article number131890
JournalTetrahedron
Volume82
Early online date28 Dec 2020
DOIs
Publication statusPublished - 26 Feb 2021

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