Coumarin-based fluorescent 'AND' logic gate probes for the detection of homocysteine and a chosen biological analyte

Luling Wu, Jordan E. Gardiner, Lokesh K. Kumawat, Hai Hao Han, Ruiying Guo, Xin Li, Xiao Peng He, Robert B.P. Elmes, Adam C. Sedgwick, Steven D. Bull, Tony D. James

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With this research we set out to develop a number of coumarin-based 'AND' logic fluorescence probes that were capable of detecting a chosen analyte in the presence of HCys. Probe JEG-CAB was constructed by attaching the ONOO- reactive unit, benzyl boronate ester, to a HCys/Cys reactive fluorescent probe, CAH. Similarly, the core unit CAH was functionalised with the nitroreductase (NTR) reactive p-nitrobenzyl unit to produce probe JEG-CAN. Both, JEG-CAB and JEG-CAN exhibited a significant fluorescence increase when exposed to either HCys and ONOO- (JEG-CAB) or HCys and NTR (JEG-CAN) thus demonstrating their effectiveness to function as AND logic gates for HCys and a chosen analyte.

Original languageEnglish
Pages (from-to)26425-26428
Number of pages4
JournalRSC Advances
Issue number45
Publication statusPublished - 27 Aug 2019

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)

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