Coumarin-based fluorescent 'AND' logic gate probes for the detection of homocysteine and a chosen biological analyte

Luling Wu; Jordan E. Gardiner; Lokesh K. Kumawat; Hai Hao Han; Ruiying Guo; Xin Li; Xiao Peng He; Robert B.P. Elmes; Adam C. Sedgwick; Steven D. Bull; Tony D. James

doi:10.1039/c9ra04908h

Coumarin-based fluorescent 'AND' logic gate probes for the detection of homocysteine and a chosen biological analyte

Luling Wu, Jordan E. Gardiner, Lokesh K. Kumawat, Hai Hao Han, Ruiying Guo, Xin Li, Xiao Peng He, Robert B.P. Elmes, Adam C. Sedgwick, Steven D. Bull, Tony D. James

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9 Citations (SciVal)

Abstract

With this research we set out to develop a number of coumarin-based 'AND' logic fluorescence probes that were capable of detecting a chosen analyte in the presence of HCys. Probe JEG-CAB was constructed by attaching the ONOO^- reactive unit, benzyl boronate ester, to a HCys/Cys reactive fluorescent probe, CAH. Similarly, the core unit CAH was functionalised with the nitroreductase (NTR) reactive p-nitrobenzyl unit to produce probe JEG-CAN. Both, JEG-CAB and JEG-CAN exhibited a significant fluorescence increase when exposed to either HCys and ONOO^- (JEG-CAB) or HCys and NTR (JEG-CAN) thus demonstrating their effectiveness to function as AND logic gates for HCys and a chosen analyte.

Original language	English
Pages (from-to)	26425-26428
Number of pages	4
Journal	RSC Advances
Volume	9
Issue number	45
DOIs	https://doi.org/10.1039/c9ra04908h
Publication status	Published - 27 Aug 2019

ASJC Scopus subject areas

General Chemistry
General Chemical Engineering

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title = "Coumarin-based fluorescent 'AND' logic gate probes for the detection of homocysteine and a chosen biological analyte",

abstract = "With this research we set out to develop a number of coumarin-based 'AND' logic fluorescence probes that were capable of detecting a chosen analyte in the presence of HCys. Probe JEG-CAB was constructed by attaching the ONOO- reactive unit, benzyl boronate ester, to a HCys/Cys reactive fluorescent probe, CAH. Similarly, the core unit CAH was functionalised with the nitroreductase (NTR) reactive p-nitrobenzyl unit to produce probe JEG-CAN. Both, JEG-CAB and JEG-CAN exhibited a significant fluorescence increase when exposed to either HCys and ONOO- (JEG-CAB) or HCys and NTR (JEG-CAN) thus demonstrating their effectiveness to function as AND logic gates for HCys and a chosen analyte.",

author = "Luling Wu and Gardiner, {Jordan E.} and Kumawat, {Lokesh K.} and Han, {Hai Hao} and Ruiying Guo and Xin Li and He, {Xiao Peng} and Elmes, {Robert B.P.} and Sedgwick, {Adam C.} and Bull, {Steven D.} and James, {Tony D.}",

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doi = "10.1039/c9ra04908h",

language = "English",

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journal = "RSC Advances",

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T1 - Coumarin-based fluorescent 'AND' logic gate probes for the detection of homocysteine and a chosen biological analyte

AU - Wu, Luling

AU - Gardiner, Jordan E.

AU - Kumawat, Lokesh K.

AU - Han, Hai Hao

AU - Guo, Ruiying

AU - Li, Xin

AU - He, Xiao Peng

AU - Elmes, Robert B.P.

AU - Sedgwick, Adam C.

AU - Bull, Steven D.

AU - James, Tony D.

PY - 2019/8/27

Y1 - 2019/8/27

N2 - With this research we set out to develop a number of coumarin-based 'AND' logic fluorescence probes that were capable of detecting a chosen analyte in the presence of HCys. Probe JEG-CAB was constructed by attaching the ONOO- reactive unit, benzyl boronate ester, to a HCys/Cys reactive fluorescent probe, CAH. Similarly, the core unit CAH was functionalised with the nitroreductase (NTR) reactive p-nitrobenzyl unit to produce probe JEG-CAN. Both, JEG-CAB and JEG-CAN exhibited a significant fluorescence increase when exposed to either HCys and ONOO- (JEG-CAB) or HCys and NTR (JEG-CAN) thus demonstrating their effectiveness to function as AND logic gates for HCys and a chosen analyte.

AB - With this research we set out to develop a number of coumarin-based 'AND' logic fluorescence probes that were capable of detecting a chosen analyte in the presence of HCys. Probe JEG-CAB was constructed by attaching the ONOO- reactive unit, benzyl boronate ester, to a HCys/Cys reactive fluorescent probe, CAH. Similarly, the core unit CAH was functionalised with the nitroreductase (NTR) reactive p-nitrobenzyl unit to produce probe JEG-CAN. Both, JEG-CAB and JEG-CAN exhibited a significant fluorescence increase when exposed to either HCys and ONOO- (JEG-CAB) or HCys and NTR (JEG-CAN) thus demonstrating their effectiveness to function as AND logic gates for HCys and a chosen analyte.

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DO - 10.1039/c9ra04908h

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JO - RSC Advances

JF - RSC Advances

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ER -

Coumarin-based fluorescent 'AND' logic gate probes for the detection of homocysteine and a chosen biological analyte

Abstract

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