Continuous enzymatic hydrolysis of sugar beet pectin and L-arabinose recovery within an integrated biorefinery

Max Cárdenas-Fernández; Charlotte Hamley-Bennett; David J. Leak; Gary J. Lye

doi:10.1016/j.biortech.2018.08.069

Continuous enzymatic hydrolysis of sugar beet pectin and L-arabinose recovery within an integrated biorefinery

Max Cárdenas-Fernández
, Charlotte Hamley-Bennett
, David J. Leak
, Gary J. Lye

Department of Life Sciences

University College London

Research output: Contribution to journal › Article › peer-review

21 Citations (SciVal)

Abstract

Sugar beet pulp (SBP) fractionated by steam explosion, released sugar beet pectin (SB-pectin) which was selectively hydrolysed using a novel α-L-arabinofuranosidase (AF), yielding monomeric L-arabinose (Ara) and a galacturonic acid rich backbone (GABB). AF was immobilised on an epoxy-functionalised resin with 70% overall immobilisation yield. Pretreatment of SB-pectin, to remove coloured compounds, improved the stability of the immobilised AF, allowing its reutilisation for up to 10 reaction cycles in a stirred tank reactor. Continuous hydrolysis of SB-pectin was subsequently performed using a packed bed reactor (PBR) with immobilised AF. Reactor performance was evaluated using a Design of Experiment approach. Pretreated SB-pectin hydrolysis was run for 7 consecutive days maintaining 73% of PBR performance. Continuous separation of Ara from GABB was achieved by tangential flow ultrafiltration with 92% Ara recovery. These results demonstrate the feasibility of establishing a continuous bioprocess to obtain Ara from the inexpensive SBP biomass.

Original language	English
Pages (from-to)	195-202
Number of pages	8
Journal	Bioresource Technology
Volume	269
Early online date	22 Aug 2018
DOIs	https://doi.org/10.1016/j.biortech.2018.08.069
Publication status	Published - 1 Dec 2018

Funding

The authors would like to thank the UK Engineering and Physical Sciences Research Council ( EPSRC ) for financial support of this work ( EP/K014897/1 ) as part of their Sustainable Chemical Feedstocks call. Input and advice from the project Industrial Advisory Board is also acknowledged.

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 7 Affordable and Clean Energy

Keywords

Arabinofuranosidase
Biomass pretreatment
Continuous bioprocessing
L-Arabinose
Sugar beet pectin

ASJC Scopus subject areas

Bioengineering
Environmental Engineering
Renewable Energy, Sustainability and the Environment
Waste Management and Disposal

Access to Document

10.1016/j.biortech.2018.08.069Licence: CC BY

Cite this

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title = "Continuous enzymatic hydrolysis of sugar beet pectin and L-arabinose recovery within an integrated biorefinery",

abstract = "Sugar beet pulp (SBP) fractionated by steam explosion, released sugar beet pectin (SB-pectin) which was selectively hydrolysed using a novel α-L-arabinofuranosidase (AF), yielding monomeric L-arabinose (Ara) and a galacturonic acid rich backbone (GABB). AF was immobilised on an epoxy-functionalised resin with 70\% overall immobilisation yield. Pretreatment of SB-pectin, to remove coloured compounds, improved the stability of the immobilised AF, allowing its reutilisation for up to 10 reaction cycles in a stirred tank reactor. Continuous hydrolysis of SB-pectin was subsequently performed using a packed bed reactor (PBR) with immobilised AF. Reactor performance was evaluated using a Design of Experiment approach. Pretreated SB-pectin hydrolysis was run for 7 consecutive days maintaining 73\% of PBR performance. Continuous separation of Ara from GABB was achieved by tangential flow ultrafiltration with 92\% Ara recovery. These results demonstrate the feasibility of establishing a continuous bioprocess to obtain Ara from the inexpensive SBP biomass.",

keywords = "Arabinofuranosidase, Biomass pretreatment, Continuous bioprocessing, L-Arabinose, Sugar beet pectin",

author = "Max C{\'a}rdenas-Fern{\'a}ndez and Charlotte Hamley-Bennett and Leak, \{David J.\} and Lye, \{Gary J.\}",

year = "2018",

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doi = "10.1016/j.biortech.2018.08.069",

language = "English",

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T1 - Continuous enzymatic hydrolysis of sugar beet pectin and L-arabinose recovery within an integrated biorefinery

AU - Cárdenas-Fernández, Max

AU - Hamley-Bennett, Charlotte

AU - Leak, David J.

AU - Lye, Gary J.

PY - 2018/12/1

Y1 - 2018/12/1

N2 - Sugar beet pulp (SBP) fractionated by steam explosion, released sugar beet pectin (SB-pectin) which was selectively hydrolysed using a novel α-L-arabinofuranosidase (AF), yielding monomeric L-arabinose (Ara) and a galacturonic acid rich backbone (GABB). AF was immobilised on an epoxy-functionalised resin with 70% overall immobilisation yield. Pretreatment of SB-pectin, to remove coloured compounds, improved the stability of the immobilised AF, allowing its reutilisation for up to 10 reaction cycles in a stirred tank reactor. Continuous hydrolysis of SB-pectin was subsequently performed using a packed bed reactor (PBR) with immobilised AF. Reactor performance was evaluated using a Design of Experiment approach. Pretreated SB-pectin hydrolysis was run for 7 consecutive days maintaining 73% of PBR performance. Continuous separation of Ara from GABB was achieved by tangential flow ultrafiltration with 92% Ara recovery. These results demonstrate the feasibility of establishing a continuous bioprocess to obtain Ara from the inexpensive SBP biomass.

AB - Sugar beet pulp (SBP) fractionated by steam explosion, released sugar beet pectin (SB-pectin) which was selectively hydrolysed using a novel α-L-arabinofuranosidase (AF), yielding monomeric L-arabinose (Ara) and a galacturonic acid rich backbone (GABB). AF was immobilised on an epoxy-functionalised resin with 70% overall immobilisation yield. Pretreatment of SB-pectin, to remove coloured compounds, improved the stability of the immobilised AF, allowing its reutilisation for up to 10 reaction cycles in a stirred tank reactor. Continuous hydrolysis of SB-pectin was subsequently performed using a packed bed reactor (PBR) with immobilised AF. Reactor performance was evaluated using a Design of Experiment approach. Pretreated SB-pectin hydrolysis was run for 7 consecutive days maintaining 73% of PBR performance. Continuous separation of Ara from GABB was achieved by tangential flow ultrafiltration with 92% Ara recovery. These results demonstrate the feasibility of establishing a continuous bioprocess to obtain Ara from the inexpensive SBP biomass.

KW - Arabinofuranosidase

KW - Biomass pretreatment

KW - Continuous bioprocessing

KW - L-Arabinose

KW - Sugar beet pectin

UR - https://www.scopus.com/pages/publications/85052473467

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DO - 10.1016/j.biortech.2018.08.069

M3 - Article

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SN - 0960-8524

VL - 269

SP - 195

EP - 202

JO - Bioresource Technology

JF - Bioresource Technology

ER -

Continuous enzymatic hydrolysis of sugar beet pectin and L-arabinose recovery within an integrated biorefinery

Abstract

Funding

UN SDGs

Keywords

ASJC Scopus subject areas

Access to Document

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Guava EasyCyte 6-2L System

Cite this

Continuous enzymatic hydrolysis of sugar beet pectin and L-arabinose recovery within an integrated biorefinery

Abstract

Funding

UN SDGs

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Equipment

Guava EasyCyte 6-2L System

Cite this