Continuous enzymatic hydrolysis of sugar beet pectin and L-arabinose recovery within an integrated biorefinery

Max Cárdenas-Fernández, Charlotte Hamley-Bennett, David J. Leak, Gary J. Lye

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Sugar beet pulp (SBP) fractionated by steam explosion, released sugar beet pectin (SB-pectin) which was selectively hydrolysed using a novel α-L-arabinofuranosidase (AF), yielding monomeric L-arabinose (Ara) and a galacturonic acid rich backbone (GABB). AF was immobilised on an epoxy-functionalised resin with 70% overall immobilisation yield. Pretreatment of SB-pectin, to remove coloured compounds, improved the stability of the immobilised AF, allowing its reutilisation for up to 10 reaction cycles in a stirred tank reactor. Continuous hydrolysis of SB-pectin was subsequently performed using a packed bed reactor (PBR) with immobilised AF. Reactor performance was evaluated using a Design of Experiment approach. Pretreated SB-pectin hydrolysis was run for 7 consecutive days maintaining 73% of PBR performance. Continuous separation of Ara from GABB was achieved by tangential flow ultrafiltration with 92% Ara recovery. These results demonstrate the feasibility of establishing a continuous bioprocess to obtain Ara from the inexpensive SBP biomass.

Original languageEnglish
Pages (from-to)195-202
Number of pages8
JournalBioresource Technology
Volume269
Early online date22 Aug 2018
DOIs
Publication statusPublished - 1 Dec 2018

Fingerprint

Sugar beets
Arabinose
Enzymatic hydrolysis
sugar beet
hydrolysis
Recovery
Packed beds
Pulp
Hydrolysis
Acids
acid
Steam
Ultrafiltration
ultrafiltration
Epoxy resins
Design of experiments
immobilization
Explosions
pectin
explosion

Keywords

  • Arabinofuranosidase
  • Biomass pretreatment
  • Continuous bioprocessing
  • L-Arabinose
  • Sugar beet pectin

ASJC Scopus subject areas

  • Bioengineering
  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal

Cite this

Continuous enzymatic hydrolysis of sugar beet pectin and L-arabinose recovery within an integrated biorefinery. / Cárdenas-Fernández, Max; Hamley-Bennett, Charlotte; Leak, David J.; Lye, Gary J.

In: Bioresource Technology, Vol. 269, 01.12.2018, p. 195-202.

Research output: Contribution to journalArticle

Cárdenas-Fernández, Max ; Hamley-Bennett, Charlotte ; Leak, David J. ; Lye, Gary J. / Continuous enzymatic hydrolysis of sugar beet pectin and L-arabinose recovery within an integrated biorefinery. In: Bioresource Technology. 2018 ; Vol. 269. pp. 195-202.
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