Construction and characterization of a highly stable human: rodent monochromosomal hybrid panel for genetic complementation and genome mapping studies

A. P. Cuthbert, D. A. Trott, R. M. Ekong, S. Jezzard, N. L. England, M. Themis, C. M. Todd, R. F. Newbold

Research output: Contribution to journalArticle

Abstract

Human:rodent somatic cell hybrids carrying a single, intact, selectable human chromosome are valuable both for functional somatic cell genetic analysis and genome mapping procedures. Here, we describe the construction and detailed been tagged with a selectable fusion gene (Hytk) introduced into the chromosome in a small (4.2 kbp) retroviral vector. Use of the Hytk marker permits both positive and negative ('in-out') selection to be applied to the human chromosome in any mammalian cell background. The panel includes 18 new hybrids isolated by direct microcell transfer from normal human diploid fibroblasts into mouse A9 cells. Molecular cytogenetic characterization of a panel of 23 stable hybrids, representing all 22 human autosomes plus the X-chromosome. Individual normal human chromosomes have been tagged with a selectable fusion gene (Hytk) introduced into the chromosome in a small (4.2 kbp) retroviral vector. Use of the Hytk marker permits both positive and negative ('inout') selection to be applied to the human chromosome in any mammalian cell background. The panel includes 18 new hybrids isolated by direct microcell transfer from normal human diploid fibroblasts into mouse A9 cells.
Original languageEnglish
Pages (from-to)68-76
Number of pages9
JournalCytogenetics and Cell Genetics
Volume71
Issue number1
DOIs
Publication statusPublished - 23 Apr 1995

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Keywords

  • animal cell
  • article
  • chromosome
  • gene mapping
  • genetic complementation
  • human
  • human cell
  • hybrid cell
  • in situ hybridization
  • mouse
  • nonhuman
  • priority journal
  • procedures

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