Conservation of proteo-lipid nuclear membrane fusion machinery during early embryogenesis

Richard D Byrne; Selvaraju Veeriah; Christopher J Applebee; Banafshé Larijani

doi:10.4161/nucl.34422

Conservation of proteo-lipid nuclear membrane fusion machinery during early embryogenesis

Richard D Byrne, Selvaraju Veeriah, Christopher J Applebee, Banafshé Larijani

Department of Life Sciences

Research output: Contribution to journal › Article › peer-review

6 Citations (SciVal)

Abstract

The fusogenic lipid diacylglycerol is essential for remodeling gamete and zygote nuclear envelopes (NE) during early embryogenesis. It is unclear whether upstream signaling molecules are likewise conserved. Here we demonstrate PLCγ and its activator SFK1, which co-operate during male pronuclear envelope formation, also promote the subsequent male and female pronuclear fusion. PLCγ and SFK1 interact directly at the fusion site leading to PLCγ activation. This is accompanied by a spatially restricted reduction of PtdIns(4,5)P2. Consequently, pronuclear fusion is blocked by PLCγ or SFK1 inhibition. These findings identify new regulators of events in the early embryo and suggest a conserved "toolkit" of fusion machinery drives successive NE fusion events during embryogenesis.

Original language	English
Pages (from-to)	441-448
Number of pages	8
Journal	Nucleus
Volume	5
Issue number	5
DOIs	https://doi.org/10.4161/nucl.34422
Publication status	Published - 9 Dec 2014

Keywords

Animals
Cell Nucleus/physiology
Embryonic Development
Female
Humans
Lipid Metabolism/genetics
Male
Membrane Fusion/genetics
Nuclear Envelope/genetics
Phosphatidylinositol 4,5-Diphosphate/metabolism
Phospholipase C gamma/genetics
Sea Urchins/embryology
Zygote/metabolism

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10.4161/nucl.34422

Cite this

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title = "Conservation of proteo-lipid nuclear membrane fusion machinery during early embryogenesis",

abstract = "The fusogenic lipid diacylglycerol is essential for remodeling gamete and zygote nuclear envelopes (NE) during early embryogenesis. It is unclear whether upstream signaling molecules are likewise conserved. Here we demonstrate PLCγ and its activator SFK1, which co-operate during male pronuclear envelope formation, also promote the subsequent male and female pronuclear fusion. PLCγ and SFK1 interact directly at the fusion site leading to PLCγ activation. This is accompanied by a spatially restricted reduction of PtdIns(4,5)P2. Consequently, pronuclear fusion is blocked by PLCγ or SFK1 inhibition. These findings identify new regulators of events in the early embryo and suggest a conserved {"}toolkit{"} of fusion machinery drives successive NE fusion events during embryogenesis. ",

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author = "Byrne, {Richard D} and Selvaraju Veeriah and Applebee, {Christopher J} and Banafsh{\'e} Larijani",

year = "2014",

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doi = "10.4161/nucl.34422",

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AU - Byrne, Richard D

AU - Veeriah, Selvaraju

AU - Applebee, Christopher J

AU - Larijani, Banafshé

PY - 2014/12/9

Y1 - 2014/12/9

N2 - The fusogenic lipid diacylglycerol is essential for remodeling gamete and zygote nuclear envelopes (NE) during early embryogenesis. It is unclear whether upstream signaling molecules are likewise conserved. Here we demonstrate PLCγ and its activator SFK1, which co-operate during male pronuclear envelope formation, also promote the subsequent male and female pronuclear fusion. PLCγ and SFK1 interact directly at the fusion site leading to PLCγ activation. This is accompanied by a spatially restricted reduction of PtdIns(4,5)P2. Consequently, pronuclear fusion is blocked by PLCγ or SFK1 inhibition. These findings identify new regulators of events in the early embryo and suggest a conserved "toolkit" of fusion machinery drives successive NE fusion events during embryogenesis.

AB - The fusogenic lipid diacylglycerol is essential for remodeling gamete and zygote nuclear envelopes (NE) during early embryogenesis. It is unclear whether upstream signaling molecules are likewise conserved. Here we demonstrate PLCγ and its activator SFK1, which co-operate during male pronuclear envelope formation, also promote the subsequent male and female pronuclear fusion. PLCγ and SFK1 interact directly at the fusion site leading to PLCγ activation. This is accompanied by a spatially restricted reduction of PtdIns(4,5)P2. Consequently, pronuclear fusion is blocked by PLCγ or SFK1 inhibition. These findings identify new regulators of events in the early embryo and suggest a conserved "toolkit" of fusion machinery drives successive NE fusion events during embryogenesis.

KW - Animals

KW - Cell Nucleus/physiology

KW - Embryonic Development

KW - Female

KW - Humans

KW - Lipid Metabolism/genetics

KW - Male

KW - Membrane Fusion/genetics

KW - Nuclear Envelope/genetics

KW - Phosphatidylinositol 4,5-Diphosphate/metabolism

KW - Phospholipase C gamma/genetics

KW - Sea Urchins/embryology

KW - Zygote/metabolism

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DO - 10.4161/nucl.34422

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VL - 5

SP - 441

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JO - Nucleus

JF - Nucleus

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ER -

Conservation of proteo-lipid nuclear membrane fusion machinery during early embryogenesis

Abstract

Keywords

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