Conservation of proteo-lipid nuclear membrane fusion machinery during early embryogenesis

Richard D Byrne, Selvaraju Veeriah, Christopher J Applebee, Banafshé Larijani

Research output: Contribution to journalArticlepeer-review

6 Citations (SciVal)


The fusogenic lipid diacylglycerol is essential for remodeling gamete and zygote nuclear envelopes (NE) during early embryogenesis. It is unclear whether upstream signaling molecules are likewise conserved. Here we demonstrate PLCγ and its activator SFK1, which co-operate during male pronuclear envelope formation, also promote the subsequent male and female pronuclear fusion. PLCγ and SFK1 interact directly at the fusion site leading to PLCγ activation. This is accompanied by a spatially restricted reduction of PtdIns(4,5)P2. Consequently, pronuclear fusion is blocked by PLCγ or SFK1 inhibition. These findings identify new regulators of events in the early embryo and suggest a conserved "toolkit" of fusion machinery drives successive NE fusion events during embryogenesis.

Original languageEnglish
Pages (from-to)441-448
Number of pages8
Issue number5
Publication statusPublished - 9 Dec 2014


  • Animals
  • Cell Nucleus/physiology
  • Embryonic Development
  • Female
  • Humans
  • Lipid Metabolism/genetics
  • Male
  • Membrane Fusion/genetics
  • Nuclear Envelope/genetics
  • Phosphatidylinositol 4,5-Diphosphate/metabolism
  • Phospholipase C gamma/genetics
  • Sea Urchins/embryology
  • Zygote/metabolism


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