Confocal and fluorescence lifetime imaging sheds light on the fate of a pyrene-tagged carbon monoxide-releasing Fischer carbene chromium complex

Rory L. Arrowsmith, Anthony J. Atkin, Stanley W. Botchway, Ian J. S. Fairlamb, Jason M. Lynam, James W. B. Moir, Sofia I. Pascu, Jonathan S. Ward, Wei-Qiang Zhang

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Abstract

The synthesis of a new pyrene-containing Fischer carbene complex is described. The complex has a broad absorbance spectrum between 300 and 400 nm and, on excitation at 345 nm in CH2Cl2 solution, emission is observed at 395 and 415 nm. Emission is also observed in PBS buffer, but in this case the resulting spectra are much broader. Confocal and fluorescence lifetime imaging indicate that emission occurs on treating HeLa cells with the complex and co-localisation studies demonstrate that this is from the mitochondria and lipid-rich regions of the cell.
Original languageEnglish
Pages (from-to)4957-4962
Number of pages6
JournalDalton Transactions
Volume44
Issue number11
Early online date4 Dec 2014
DOIs
Publication statusPublished - 21 Mar 2015

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    Arrowsmith, R. L., Atkin, A. J., Botchway, S. W., Fairlamb, I. J. S., Lynam, J. M., Moir, J. W. B., Pascu, S. I., Ward, J. S., & Zhang, W-Q. (2015). Confocal and fluorescence lifetime imaging sheds light on the fate of a pyrene-tagged carbon monoxide-releasing Fischer carbene chromium complex. Dalton Transactions , 44(11), 4957-4962. https://doi.org/10.1039/C4DT03312D