@article{5a9c8eb24f754967be83520d990ab7d6,
title = "Computational Fluorescence Suppression in Shifted Excitation Raman Spectroscopy",
abstract = "Fiber-based Raman spectroscopy in the context of in vivo biomedical application suffers from the presence of background fluorescence from the surrounding tissue that might mask the crucial but inherently weak Raman signatures. One method that has shown potential for suppressing the background to reveal the Raman spectra is shifted excitation Raman spectroscopy (SER). SER collects multiple emission spectra by shifting the excitation by small amounts and uses these spectra to computationally suppress the fluorescence background based on the principle that Raman spectrum shifts with excitation while fluorescence spectrum does not. We introduce a method that utilizes the spectral characteristics of the Raman and fluorescence spectra to estimate them more effectively, and compare this approach against existing methods on real world datasets.",
keywords = "Biomedical, Fluorescence, Lung, Lung Tissue, Machine Learning, Microscopy, Optical Fiber, Optical fibers, Optical scattering, Raman scattering, Raman Spectroscopy, Regularization, Shifted Excitation, Smoothing methods, Smoothness,, Sparsity, Spectroscopy",
author = "Jenkins, {Nia C.} and Katjana Ehrlich and Andras Kufcsak and Stephanos Yerolatsitis and Susan Fernandes and Irene Young and Katie Hamilton and Wood, {Harry A.C.} and Tom Quinn and Vikki Young and Akram, {Ahsan R.} and Stone, {James M.} and Thomson, {Robert R.} and Keith Finlayson and Kevin Dhaliwal and Sohan Seth",
note = "The authors thank the BioResource for access to tissue (NHS Lothian BioResource, Scotland Research Ethics Service, reference 15/ES/0094). NCJ was supported through the University of Edinburgh funding award. JMS, SY and HACW are supported through an EPSRC fellowship (EP/S001123/1). SF is supported through an MRC fellowship (MR/R017794/1). ARA is supported by a CRUK Clinician Scientist Fellowship (A24867). NCJ and SS are with the School of Informatics, University of Edinburgh, 10 Crichton Street, Edinburgh, EH8 9AB, UK. (e-mails: {njenkin3,sohan.seth}@ed.ac.uk). KE, AK, SF, IY, KH, TQ, VY, ARA, RRT, KF, KD, and SS are with the Translational Healthcare Technology Group, Centre for Inflammation Research, Queen{\textquoteright}s Medical Research Institute, University of Edinburgh, 47 Little France Crescent, Edinburgh, EH16 4TJ, UK. (e-mails: {Katjana.Ehrlich,A.Kufcsak,Susan.Fernandes, iyoung3 K.Hamilton,tquinn,Vikki.Young,Ahsan.Akram,Keith.Finlayson, Kev.Dhaliwal}@ed.ac.uk). SY, HACW. and JMS are with the Centre for Photonics and Photonic Materials, University of Bath, Claverton Down, Bath, BA2 7AY, UK. (emails: {S.Yerolatsitis,H.Wood,J.M.Stone}@bath.ac.uk). RRT is with Scottish Universities Physics Alliance (SUPA), Institute of Photonics and Quantum Science, Heriot-Watt University, Edinburgh, EH144AS, UK. (email: R.R.Thomson@hw.ac.uk). ",
year = "2023",
month = feb,
day = "10",
doi = "10.1109/TBME.2023.3243866",
language = "English",
pages = "1--18",
journal = "IEEE Transactions on biomedical engineering",
issn = "0018-9294",
publisher = "IEEE",
}