TY - JOUR
T1 - Complete structures of Bordetella bronchiseptica and Bordetella parapertussis lipopolysaccharides
AU - Preston, Andrew
AU - Petersen, Bent O
AU - Duus, Jens Ø
AU - Kubler-Kielb, Joanna
AU - Ben-Menachem, Gil
AU - Li, Jianjun
AU - Vinogradov, Evgeny
PY - 2006
Y1 - 2006
N2 - The structures of the lipopolysaccharide (LPS) core and O antigen of Bordetella bronchiseptica and Bordetella parapertussis are known, but how these two regions are linked to each other had not been determined. We have studied LPS from several strains of these microorganisms to determine the complete carbohydrate structure of the LPS. LPS was analyzed using different chemical degradations, NMR spectroscopy, and mass spectrometry. This identified a novel pentasaccharide fragment that links the O chain to the core in all the LPS studied. In addition, although the O chain of these bacteria was reported as a homopolymer of 1,4-linked 2,3-diacetamido-2,3-dideoxy-alpha-galacturonic acid, we discovered that the polymer contains several amidated uronic acids, the number of which varies between strains. These new data describe the complete structure of the LPS carbohydrate backbone for both Bordetella species and help to explain the complex genetics of LPS biosynthesis in these bacteria.
AB - The structures of the lipopolysaccharide (LPS) core and O antigen of Bordetella bronchiseptica and Bordetella parapertussis are known, but how these two regions are linked to each other had not been determined. We have studied LPS from several strains of these microorganisms to determine the complete carbohydrate structure of the LPS. LPS was analyzed using different chemical degradations, NMR spectroscopy, and mass spectrometry. This identified a novel pentasaccharide fragment that links the O chain to the core in all the LPS studied. In addition, although the O chain of these bacteria was reported as a homopolymer of 1,4-linked 2,3-diacetamido-2,3-dideoxy-alpha-galacturonic acid, we discovered that the polymer contains several amidated uronic acids, the number of which varies between strains. These new data describe the complete structure of the LPS carbohydrate backbone for both Bordetella species and help to explain the complex genetics of LPS biosynthesis in these bacteria.
UR - http://dx.doi.org/10.1074/jbc.M513904200
U2 - 10.1074/jbc.M513904200
DO - 10.1074/jbc.M513904200
M3 - Article
C2 - 16632471
SN - 0021-9258
VL - 281
SP - 18135
EP - 18144
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 26
ER -