Comparative genomic survey of Bacillus cereus sensu stricto isolates from the dairy production chain in Brazil

Gabriel Augusto Marques Rossi; Higor Oliveira Silva; Carlos Eduardo Gamero Aguilar; Arina Lázaro Rochetti; Ben Pascoe; Guillaume Méric; Evangelos Mourkas; Matthew D. Hitchings; Luis Antonio Mathias; Vera Letticie De Azevedo Ruiz; Heidge Fukumasu; Samuel K. Sheppard; Ana Maria Centola Vidal

doi:10.1093/femsle/fnx283

Comparative genomic survey of Bacillus cereus sensu stricto isolates from the dairy production chain in Brazil

Gabriel Augusto Marques Rossi, Higor Oliveira Silva, Carlos Eduardo Gamero Aguilar, Arina Lázaro Rochetti, Ben Pascoe, Guillaume Méric, Evangelos Mourkas, Matthew D. Hitchings, Luis Antonio Mathias, Vera Letticie De Azevedo Ruiz, Heidge Fukumasu, Samuel K. Sheppard, Ana Maria Centola Vidal

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Abstract

The genomes of 262 Bacillus cereus isolates were analyzed including 69 isolates sampled from equipment, raw milk and dairy products from Brazil. The population structure of isolates showed strains belonging to known phylogenetic groups II, III, IV, V and VI. Almost all the isolates obtained from dairy products belonged to group III. Investigation of specific alleles revealed high numbers of isolates carrying toxin-associated genes including cytK (53.62%), hblA (59.42%), hblC (44.93%), hblD (53.62%), nheA (84.06%), nheB (89.86%) and nheC (84.06%) with isolates belonging to groups IV and V having significant higher prevalence of hblACD and group IV of CytK genes. Strains from dairy products had significantly lower prevalence of CytK and hblACD genes compared to isolates from equipment and raw milk/bulk tanks. Genes related to sucrose metabolism were detected at higher frequency in isolates obtained from raw milk compared to strains from equipment and utensils. The population genomic analysis demonstrated the diversity of strains and variability of putative function among B. cereus group isolates in Brazilian dairy production, with large numbers of strains potentially able to cause foodborne illness. This detailed information will contribute to targeted interventions to reduce milk contamination and spoilage associated with B. cereus in Brazil.

Original language	English
Article number	fnx283
Pages (from-to)	1 - 9
Number of pages	9
Journal	FEMS Microbiology Letters
Volume	365
Issue number	3
Early online date	27 Dec 2017
DOIs	https://doi.org/10.1093/femsle/fnx283
Publication status	Published - 1 Feb 2018

Bibliographical note

Keywords

Foodborne diseases
Milk
Next-generation sequencing
Population genomics

ASJC Scopus subject areas

Microbiology
Molecular Biology
Genetics

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10.1093/femsle/fnx283

2017_12_Rossi_et_al_GM_EM_BP_MDH_SKS_Dairy_Bacillus_in_Brazil_FEMS
This is a pre-copyedited, author-produced version of an article accepted for publication in FEMS Microbiology Letters following peer review. The version of record, Gabriel Augusto Marques Rossi, Higor Oliveira Silva, Carlos Eduardo Gamero Aguilar, Arina Lázaro Rochetti, Ben Pascoe, Guillaume Méric, Evangelos Mourkas, Matthew D Hitchings, Luis Antonio Mathias, Vera Letticie de Azevedo Ruiz, Heidge Fukumasu, Samuel K Sheppard, Ana Maria Centola Vidal; Comparative genomic survey of Bacillus cereus sensu stricto isolates from the dairy production chain in Brazil, FEMS Microbiology Letters, Volume 365, Issue 3, 1 February 2018, fnx283, is available online at: https://doi.org/10.1093/femsle/fnx283
Accepted author manuscript, 739 KB

Cite this

Marques Rossi, G. A., Silva, H. O., Gamero Aguilar, C. E., Rochetti, A. L., Pascoe, B., Méric, G., Mourkas, E., Hitchings, M. D., Mathias, L. A., De Azevedo Ruiz, V. L., Fukumasu, H., Sheppard, S. K., & Centola Vidal, A. M. (2018). Comparative genomic survey of Bacillus cereus sensu stricto isolates from the dairy production chain in Brazil. FEMS Microbiology Letters, 365(3), 1 - 9. Article fnx283. https://doi.org/10.1093/femsle/fnx283

Marques Rossi, GA, Silva, HO, Gamero Aguilar, CE, Rochetti, AL, Pascoe, B, Méric, G, Mourkas, E, Hitchings, MD, Mathias, LA, De Azevedo Ruiz, VL, Fukumasu, H, Sheppard, SK & Centola Vidal, AM 2018, 'Comparative genomic survey of Bacillus cereus sensu stricto isolates from the dairy production chain in Brazil', FEMS Microbiology Letters, vol. 365, no. 3, fnx283, pp. 1 - 9. https://doi.org/10.1093/femsle/fnx283

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Comparative genomic survey of Bacillus cereus sensu stricto isolates from the dairy production chain in Brazil

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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