Comparative genomic survey of Bacillus cereus sensu stricto isolates from the dairy production chain in Brazil

Gabriel Augusto Marques Rossi, Higor Oliveira Silva, Carlos Eduardo Gamero Aguilar, Arina Lázaro Rochetti, Ben Pascoe, Guillaume Méric, Evangelos Mourkas, Matthew D. Hitchings, Luis Antonio Mathias, Vera Letticie De Azevedo Ruiz, Heidge Fukumasu, Samuel K. Sheppard, Ana Maria Centola Vidal

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24 Downloads (Pure)

Abstract

The genomes of 262 Bacillus cereus isolates were analyzed including 69 isolates sampled from equipment, raw milk and dairy products from Brazil. The population structure of isolates showed strains belonging to known phylogenetic groups II, III, IV, V and VI. Almost all the isolates obtained from dairy products belonged to group III. Investigation of specific alleles revealed high numbers of isolates carrying toxin-associated genes including cytK (53.62%), hblA (59.42%), hblC (44.93%), hblD (53.62%), nheA (84.06%), nheB (89.86%) and nheC (84.06%) with isolates belonging to groups IV and V having significant higher prevalence of hblACD and group IV of CytK genes. Strains from dairy products had significantly lower prevalence of CytK and hblACD genes compared to isolates from equipment and raw milk/bulk tanks. Genes related to sucrose metabolism were detected at higher frequency in isolates obtained from raw milk compared to strains from equipment and utensils. The population genomic analysis demonstrated the diversity of strains and variability of putative function among B. cereus group isolates in Brazilian dairy production, with large numbers of strains potentially able to cause foodborne illness. This detailed information will contribute to targeted interventions to reduce milk contamination and spoilage associated with B. cereus in Brazil.

Original languageEnglish
Article numberfnx283
Pages (from-to)1 - 9
Number of pages9
JournalFEMS Microbiology Letters
Volume365
Issue number3
Early online date27 Dec 2017
DOIs
Publication statusPublished - 1 Feb 2018

Fingerprint

Bacillus cereus
Brazil
Dairy Products
Milk
Equipment and Supplies
Genes
Metagenomics
Foodborne Diseases
Sucrose
Alleles
Genome
Surveys and Questionnaires
Population

Keywords

  • Foodborne diseases
  • Milk
  • Next-generation sequencing
  • Population genomics

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Genetics

Cite this

Marques Rossi, G. A., Silva, H. O., Gamero Aguilar, C. E., Rochetti, A. L., Pascoe, B., Méric, G., ... Centola Vidal, A. M. (2018). Comparative genomic survey of Bacillus cereus sensu stricto isolates from the dairy production chain in Brazil. FEMS Microbiology Letters, 365(3), 1 - 9. [fnx283]. https://doi.org/10.1093/femsle/fnx283, https://doi.org/10.1093/femsle/fnx283

Comparative genomic survey of Bacillus cereus sensu stricto isolates from the dairy production chain in Brazil. / Marques Rossi, Gabriel Augusto; Silva, Higor Oliveira; Gamero Aguilar, Carlos Eduardo; Rochetti, Arina Lázaro; Pascoe, Ben; Méric, Guillaume; Mourkas, Evangelos; Hitchings, Matthew D.; Mathias, Luis Antonio; De Azevedo Ruiz, Vera Letticie; Fukumasu, Heidge; Sheppard, Samuel K.; Centola Vidal, Ana Maria.

In: FEMS Microbiology Letters, Vol. 365, No. 3, fnx283, 01.02.2018, p. 1 - 9.

Research output: Contribution to journalArticle

Marques Rossi, GA, Silva, HO, Gamero Aguilar, CE, Rochetti, AL, Pascoe, B, Méric, G, Mourkas, E, Hitchings, MD, Mathias, LA, De Azevedo Ruiz, VL, Fukumasu, H, Sheppard, SK & Centola Vidal, AM 2018, 'Comparative genomic survey of Bacillus cereus sensu stricto isolates from the dairy production chain in Brazil', FEMS Microbiology Letters, vol. 365, no. 3, fnx283, pp. 1 - 9. https://doi.org/10.1093/femsle/fnx283, https://doi.org/10.1093/femsle/fnx283
Marques Rossi, Gabriel Augusto ; Silva, Higor Oliveira ; Gamero Aguilar, Carlos Eduardo ; Rochetti, Arina Lázaro ; Pascoe, Ben ; Méric, Guillaume ; Mourkas, Evangelos ; Hitchings, Matthew D. ; Mathias, Luis Antonio ; De Azevedo Ruiz, Vera Letticie ; Fukumasu, Heidge ; Sheppard, Samuel K. ; Centola Vidal, Ana Maria. / Comparative genomic survey of Bacillus cereus sensu stricto isolates from the dairy production chain in Brazil. In: FEMS Microbiology Letters. 2018 ; Vol. 365, No. 3. pp. 1 - 9.
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N2 - The genomes of 262 Bacillus cereus isolates were analyzed including 69 isolates sampled from equipment, raw milk and dairy products from Brazil. The population structure of isolates showed strains belonging to known phylogenetic groups II, III, IV, V and VI. Almost all the isolates obtained from dairy products belonged to group III. Investigation of specific alleles revealed high numbers of isolates carrying toxin-associated genes including cytK (53.62%), hblA (59.42%), hblC (44.93%), hblD (53.62%), nheA (84.06%), nheB (89.86%) and nheC (84.06%) with isolates belonging to groups IV and V having significant higher prevalence of hblACD and group IV of CytK genes. Strains from dairy products had significantly lower prevalence of CytK and hblACD genes compared to isolates from equipment and raw milk/bulk tanks. Genes related to sucrose metabolism were detected at higher frequency in isolates obtained from raw milk compared to strains from equipment and utensils. The population genomic analysis demonstrated the diversity of strains and variability of putative function among B. cereus group isolates in Brazilian dairy production, with large numbers of strains potentially able to cause foodborne illness. This detailed information will contribute to targeted interventions to reduce milk contamination and spoilage associated with B. cereus in Brazil.

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