Community sewage sensors towards evaluation of drug use trends

detection of cocaine in wastewater with DNA-directed immobilization aptamer sensors

Zhugen Yang, Erika Castrignano, Pedro Estrela, Christopher Frost, Barbara Kasprzyk-Hordern

Research output: Contribution to journalArticle

16 Citations (Scopus)
50 Downloads (Pure)

Abstract

Illicit drug use has a global concern and effective monitoring and interventions is highly required to combat drug abuse. Wastewater-based epidemiology (WBE) is an innovative and cost-effective approach to evaluate community-wide drug use trends, compared to traditional population surveys. Here we report for the first time, a novel quantitative community sewage sensor (namely DNA-directed immobilization of aptamer sensors, DDIAS) for rapid and cost-effective estimation of cocaine use trends via WBE. Thiolated single-stranded DNA (ssDNA) probe was hybridized with aptamer ssDNA in solution, followed by co-immobilization with 6-mercapto-hexane onto the gold electrodes to control the surface density to effectively bind with cocaine. DDIAS was optimized to detect cocaine at as low as 10 nM with a dynamic range from 10 nM to 5 μM, which were further employed for the quantification of cocaine in wastewater samples collected from a wastewater treatment plant in seven consecutive days. The concentration pattern of the sampling week is comparable with that from mass spectrometry. Our results demonstrate that the developed DDIAS can be used as community sewage sensors for rapid and cost-effective evaluation of drug use trends, and potentially implemented as a powerful tool for on-site and real-time monitoring of wastewater by un-skilled personnel.
Original languageEnglish
Article number21024
Pages (from-to)1-10
Number of pages10
JournalScientific Reports
Volume6
DOIs
Publication statusPublished - 15 Feb 2016

Fingerprint

Sewage
Cocaine
Wastewater
DNA
Sensors
Epidemiology
Pharmaceutical Preparations
Single-Stranded DNA
Costs
Monitoring
DNA Probes
Street Drugs
Hexanes
Gold
Wastewater treatment
Mass spectrometry
Personnel
Sampling
Electrodes

Cite this

@article{39a953058e9e4f64a686dbd03287dda8,
title = "Community sewage sensors towards evaluation of drug use trends: detection of cocaine in wastewater with DNA-directed immobilization aptamer sensors",
abstract = "Illicit drug use has a global concern and effective monitoring and interventions is highly required to combat drug abuse. Wastewater-based epidemiology (WBE) is an innovative and cost-effective approach to evaluate community-wide drug use trends, compared to traditional population surveys. Here we report for the first time, a novel quantitative community sewage sensor (namely DNA-directed immobilization of aptamer sensors, DDIAS) for rapid and cost-effective estimation of cocaine use trends via WBE. Thiolated single-stranded DNA (ssDNA) probe was hybridized with aptamer ssDNA in solution, followed by co-immobilization with 6-mercapto-hexane onto the gold electrodes to control the surface density to effectively bind with cocaine. DDIAS was optimized to detect cocaine at as low as 10 nM with a dynamic range from 10 nM to 5 μM, which were further employed for the quantification of cocaine in wastewater samples collected from a wastewater treatment plant in seven consecutive days. The concentration pattern of the sampling week is comparable with that from mass spectrometry. Our results demonstrate that the developed DDIAS can be used as community sewage sensors for rapid and cost-effective evaluation of drug use trends, and potentially implemented as a powerful tool for on-site and real-time monitoring of wastewater by un-skilled personnel.",
author = "Zhugen Yang and Erika Castrignano and Pedro Estrela and Christopher Frost and Barbara Kasprzyk-Hordern",
year = "2016",
month = "2",
day = "15",
doi = "10.1038/srep21024",
language = "English",
volume = "6",
pages = "1--10",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Research",

}

TY - JOUR

T1 - Community sewage sensors towards evaluation of drug use trends

T2 - detection of cocaine in wastewater with DNA-directed immobilization aptamer sensors

AU - Yang, Zhugen

AU - Castrignano, Erika

AU - Estrela, Pedro

AU - Frost, Christopher

AU - Kasprzyk-Hordern, Barbara

PY - 2016/2/15

Y1 - 2016/2/15

N2 - Illicit drug use has a global concern and effective monitoring and interventions is highly required to combat drug abuse. Wastewater-based epidemiology (WBE) is an innovative and cost-effective approach to evaluate community-wide drug use trends, compared to traditional population surveys. Here we report for the first time, a novel quantitative community sewage sensor (namely DNA-directed immobilization of aptamer sensors, DDIAS) for rapid and cost-effective estimation of cocaine use trends via WBE. Thiolated single-stranded DNA (ssDNA) probe was hybridized with aptamer ssDNA in solution, followed by co-immobilization with 6-mercapto-hexane onto the gold electrodes to control the surface density to effectively bind with cocaine. DDIAS was optimized to detect cocaine at as low as 10 nM with a dynamic range from 10 nM to 5 μM, which were further employed for the quantification of cocaine in wastewater samples collected from a wastewater treatment plant in seven consecutive days. The concentration pattern of the sampling week is comparable with that from mass spectrometry. Our results demonstrate that the developed DDIAS can be used as community sewage sensors for rapid and cost-effective evaluation of drug use trends, and potentially implemented as a powerful tool for on-site and real-time monitoring of wastewater by un-skilled personnel.

AB - Illicit drug use has a global concern and effective monitoring and interventions is highly required to combat drug abuse. Wastewater-based epidemiology (WBE) is an innovative and cost-effective approach to evaluate community-wide drug use trends, compared to traditional population surveys. Here we report for the first time, a novel quantitative community sewage sensor (namely DNA-directed immobilization of aptamer sensors, DDIAS) for rapid and cost-effective estimation of cocaine use trends via WBE. Thiolated single-stranded DNA (ssDNA) probe was hybridized with aptamer ssDNA in solution, followed by co-immobilization with 6-mercapto-hexane onto the gold electrodes to control the surface density to effectively bind with cocaine. DDIAS was optimized to detect cocaine at as low as 10 nM with a dynamic range from 10 nM to 5 μM, which were further employed for the quantification of cocaine in wastewater samples collected from a wastewater treatment plant in seven consecutive days. The concentration pattern of the sampling week is comparable with that from mass spectrometry. Our results demonstrate that the developed DDIAS can be used as community sewage sensors for rapid and cost-effective evaluation of drug use trends, and potentially implemented as a powerful tool for on-site and real-time monitoring of wastewater by un-skilled personnel.

U2 - 10.1038/srep21024

DO - 10.1038/srep21024

M3 - Article

VL - 6

SP - 1

EP - 10

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

M1 - 21024

ER -