Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 6694-6703 |
| Number of pages | 10 |
| Journal | Applied and Environmental Microbiology |
| Volume | 80 |
| Issue number | 21 |
| Early online date | 6 Oct 2014 |
| DOIs | |
| Publication status | Published - 1 Nov 2014 |
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Keywords
- bacteriophage therapy
- biofilms
- Staphylococcus aureus
Cite this
Combined use of bacteriophage K and a novel bacteriophage to reduce Staphylococcus aureus biofilm. / Alves, Diana; Gaudion, A; Bean, Jessica; Pérez Esteban, Patricia; Arnot, T C; Harper, D R; Kot, W; Hansen, L H; Enright, M C; Jenkins, A T A.
In: Applied and Environmental Microbiology, Vol. 80, No. 21, 01.11.2014, p. 6694-6703.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Combined use of bacteriophage K and a novel bacteriophage to reduce Staphylococcus aureus biofilm
AU - Alves, Diana
AU - Gaudion, A
AU - Bean, Jessica
AU - Pérez Esteban, Patricia
AU - Arnot, T C
AU - Harper, D R
AU - Kot, W
AU - Hansen, L H
AU - Enright, M C
AU - Jenkins, A T A
PY - 2014/11/1
Y1 - 2014/11/1
N2 - Biofilms are major causes of impairment of wound healing and patient morbidity. One of the most common and aggressive wound pathogens is Staphylococcus aureus, displaying a a large repertoire of virulence factors and commonly reduced susceptibility to antibiotics, such as the spread of methicillin-resistant S. aureus (MRSA). Bacteriophages are obligate parasites of bacteria. They multiply intracellularly and lyse their bacterial host releasing their progeny. We isolated a novel phage, DRA88 that has a broad host range amongst S. aureus. Morphologically the phage belongs to the Myoviridae family and comprises a large dsDNA genome of 141,907 bp. DRA88 was mixed with phage K to produce a high titre mixture that showed strong lytic activity against a wide range of S. aureus isolates including representatives of the major international MRSA clones and coagulase-negative Staphylococcus. Its efficacy was assessed both in planktonic cultures and when treating established biofilms produced by three different biofilm producing S. aureus isolates. A significant reduction of biofilm biomass over 48 hours of treatment was recorded in all cases. The phage mixture may form the basis of an effective treatment for infectious caused by S. aureus biofilms.
AB - Biofilms are major causes of impairment of wound healing and patient morbidity. One of the most common and aggressive wound pathogens is Staphylococcus aureus, displaying a a large repertoire of virulence factors and commonly reduced susceptibility to antibiotics, such as the spread of methicillin-resistant S. aureus (MRSA). Bacteriophages are obligate parasites of bacteria. They multiply intracellularly and lyse their bacterial host releasing their progeny. We isolated a novel phage, DRA88 that has a broad host range amongst S. aureus. Morphologically the phage belongs to the Myoviridae family and comprises a large dsDNA genome of 141,907 bp. DRA88 was mixed with phage K to produce a high titre mixture that showed strong lytic activity against a wide range of S. aureus isolates including representatives of the major international MRSA clones and coagulase-negative Staphylococcus. Its efficacy was assessed both in planktonic cultures and when treating established biofilms produced by three different biofilm producing S. aureus isolates. A significant reduction of biofilm biomass over 48 hours of treatment was recorded in all cases. The phage mixture may form the basis of an effective treatment for infectious caused by S. aureus biofilms.
KW - bacteriophage therapy
KW - biofilms
KW - Staphylococcus aureus
U2 - 10.1128/AEM.01789-14
DO - 10.1128/AEM.01789-14
M3 - Article
VL - 80
SP - 6694
EP - 6703
JO - Applied and Environmental Microbiology
JF - Applied and Environmental Microbiology
SN - 0099-2240
IS - 21
ER -