Combined use of bacteriophage K and a novel bacteriophage to reduce Staphylococcus aureus biofilm

Diana Alves, A Gaudion, Jessica Bean, Patricia Pérez Esteban, T C Arnot, D R Harper, W Kot, L H Hansen, M C Enright, A T A Jenkins

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Abstract

Biofilms are major causes of impairment of wound healing and patient morbidity. One of the most common and aggressive wound pathogens is Staphylococcus aureus, displaying a a large repertoire of virulence factors and commonly reduced susceptibility to antibiotics, such as the spread of methicillin-resistant S. aureus (MRSA). Bacteriophages are obligate parasites of bacteria. They multiply intracellularly and lyse their bacterial host releasing their progeny. We isolated a novel phage, DRA88 that has a broad host range amongst S. aureus. Morphologically the phage belongs to the Myoviridae family and comprises a large dsDNA genome of 141,907 bp. DRA88 was mixed with phage K to produce a high titre mixture that showed strong lytic activity against a wide range of S. aureus isolates including representatives of the major international MRSA clones and coagulase-negative Staphylococcus. Its efficacy was assessed both in planktonic cultures and when treating established biofilms produced by three different biofilm producing S. aureus isolates. A significant reduction of biofilm biomass over 48 hours of treatment was recorded in all cases. The phage mixture may form the basis of an effective treatment for infectious caused by S. aureus biofilms.
Original languageEnglish
Pages (from-to)6694-6703
Number of pages10
JournalApplied and Environmental Microbiology
Volume80
Issue number21
Early online date6 Oct 2014
DOIs
Publication statusPublished - 1 Nov 2014

Fingerprint

bacteriophage
Biofilms
bacteriophages
Bacteriophages
biofilm
Staphylococcus aureus
Methicillin-Resistant Staphylococcus aureus
Myoviridae
coagulase negative staphylococci
Coagulase
Host Specificity
host range
lysis
Virulence Factors
morbidity
virulence
tissue repair
Staphylococcus
animal injuries
antibiotics

Keywords

  • bacteriophage therapy
  • biofilms
  • Staphylococcus aureus

Cite this

Combined use of bacteriophage K and a novel bacteriophage to reduce Staphylococcus aureus biofilm. / Alves, Diana; Gaudion, A; Bean, Jessica; Pérez Esteban, Patricia; Arnot, T C; Harper, D R; Kot, W; Hansen, L H; Enright, M C; Jenkins, A T A.

In: Applied and Environmental Microbiology, Vol. 80, No. 21, 01.11.2014, p. 6694-6703.

Research output: Contribution to journalArticle

Alves, D, Gaudion, A, Bean, J, Pérez Esteban, P, Arnot, TC, Harper, DR, Kot, W, Hansen, LH, Enright, MC & Jenkins, ATA 2014, 'Combined use of bacteriophage K and a novel bacteriophage to reduce Staphylococcus aureus biofilm', Applied and Environmental Microbiology, vol. 80, no. 21, pp. 6694-6703. https://doi.org/10.1128/AEM.01789-14
Alves, Diana ; Gaudion, A ; Bean, Jessica ; Pérez Esteban, Patricia ; Arnot, T C ; Harper, D R ; Kot, W ; Hansen, L H ; Enright, M C ; Jenkins, A T A. / Combined use of bacteriophage K and a novel bacteriophage to reduce Staphylococcus aureus biofilm. In: Applied and Environmental Microbiology. 2014 ; Vol. 80, No. 21. pp. 6694-6703.
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