Abstract
Collagen fibrils, a major component of mitral valve leaflets, play an important role in defining shape and providing mechanical strength and flexibility. Histopathological studies show that collagen fibrils undergo dramatic changes in the course of myxomatous mitral valve disease in both dogs and humans. However, little is known about the detailed organization of collagen in this disease. This study was designed to analyze and compare collagen fibril organization in healthy and lesional areas of myxomatous mitral valves of dogs, using synchrotron small-angle x-ray diffraction. The orientation, density, and alignment of collagen fibrils were mapped across six different valves. The findings reveal a preferred collagen alignment in the main body of the leaflets between two commissures. Qualitative and quantitative analysis of the data showed significant differences between affected and lesion-free areas in terms of collagen content, fibril alignment, and total tissue volume. Regression analysis of the amount of collagen compared to the total tissue content at each point revealed a significant relationship between these two parameters in lesion-free but not in affected areas. This is the first time this technique has been used to map collagen fibrils in cardiac tissue; the findings have important applications to human cardiology.
| Original language | English |
|---|---|
| Pages (from-to) | 2472-2476 |
| Number of pages | 5 |
| Journal | Biophysical Journal |
| Volume | 93 |
| Issue number | 7 |
| DOIs | |
| Publication status | Published - 1 Oct 2007 |
ASJC Scopus subject areas
- Biophysics
Cite this
Collagen organization in canine myxomatous mitral valve disease : An X-ray diffraction study. / Hadian, Mojtaba; Corcoran, Brendan M.; Han, Richard I.; Grossmann, J. Günter; Bradshaw, Jeremy P.
In: Biophysical Journal, Vol. 93, No. 7, 01.10.2007, p. 2472-2476.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Collagen organization in canine myxomatous mitral valve disease
T2 - An X-ray diffraction study
AU - Hadian, Mojtaba
AU - Corcoran, Brendan M.
AU - Han, Richard I.
AU - Grossmann, J. Günter
AU - Bradshaw, Jeremy P.
PY - 2007/10/1
Y1 - 2007/10/1
N2 - Collagen fibrils, a major component of mitral valve leaflets, play an important role in defining shape and providing mechanical strength and flexibility. Histopathological studies show that collagen fibrils undergo dramatic changes in the course of myxomatous mitral valve disease in both dogs and humans. However, little is known about the detailed organization of collagen in this disease. This study was designed to analyze and compare collagen fibril organization in healthy and lesional areas of myxomatous mitral valves of dogs, using synchrotron small-angle x-ray diffraction. The orientation, density, and alignment of collagen fibrils were mapped across six different valves. The findings reveal a preferred collagen alignment in the main body of the leaflets between two commissures. Qualitative and quantitative analysis of the data showed significant differences between affected and lesion-free areas in terms of collagen content, fibril alignment, and total tissue volume. Regression analysis of the amount of collagen compared to the total tissue content at each point revealed a significant relationship between these two parameters in lesion-free but not in affected areas. This is the first time this technique has been used to map collagen fibrils in cardiac tissue; the findings have important applications to human cardiology.
AB - Collagen fibrils, a major component of mitral valve leaflets, play an important role in defining shape and providing mechanical strength and flexibility. Histopathological studies show that collagen fibrils undergo dramatic changes in the course of myxomatous mitral valve disease in both dogs and humans. However, little is known about the detailed organization of collagen in this disease. This study was designed to analyze and compare collagen fibril organization in healthy and lesional areas of myxomatous mitral valves of dogs, using synchrotron small-angle x-ray diffraction. The orientation, density, and alignment of collagen fibrils were mapped across six different valves. The findings reveal a preferred collagen alignment in the main body of the leaflets between two commissures. Qualitative and quantitative analysis of the data showed significant differences between affected and lesion-free areas in terms of collagen content, fibril alignment, and total tissue volume. Regression analysis of the amount of collagen compared to the total tissue content at each point revealed a significant relationship between these two parameters in lesion-free but not in affected areas. This is the first time this technique has been used to map collagen fibrils in cardiac tissue; the findings have important applications to human cardiology.
UR - http://www.scopus.com/inward/record.url?scp=34848880709&partnerID=8YFLogxK
U2 - 10.1529/biophysj.107.107847
DO - 10.1529/biophysj.107.107847
M3 - Article
C2 - 17557795
AN - SCOPUS:34848880709
VL - 93
SP - 2472
EP - 2476
JO - Biophysical Journal
JF - Biophysical Journal
SN - 0006-3495
IS - 7
ER -