Cold Atmospheric Plasma-Activated Composite Hydrogel for an Enhanced and On-Demand Delivery of Antimicrobials

Nishtha Gaur; Bethany Lee Patenall; Bhagirath Ghimire; Naing Thet; Jordan Gardiner; Krystal  Le Dore; Gordon Ramage; Bryn Short; Rachel Heylen; Craig Williams; Robert Short; Andrew Jenkins

doi:10.1021/acsami.3c01208

Cold Atmospheric Plasma-Activated Composite Hydrogel for an Enhanced and On-Demand Delivery of Antimicrobials

Nishtha Gaur, Bethany Lee Patenall, Bhagirath Ghimire, Naing Thet, Jordan Gardiner, Krystal Le Dore, Gordon Ramage, Bryn Short, Rachel Heylen, Craig Williams, Robert Short, Andrew Jenkins

Research output: Contribution to journal › Article › peer-review

16 Citations (SciVal)

Abstract

We present the concept of a versatile drug-loaded composite hydrogel that can be activated using an argon-based cold atmospheric plasma (CAP) jet to deliver both a drug and CAP-generated molecules, concomitantly, in a tissue target. To demonstrate this concept, we utilized the antibiotic gentamicin that is encapsulated in sodium polyacrylate (PAA) particles, which are dispersed within a poly(vinyl alcohol) (PVA) hydrogel matrix. The final product is a gentamicin-PAA-PVA composite hydrogel suitable for an on-demand triggered release using CAP. We show that by activating using CAP, we can effectively release gentamicin from the hydrogel and also eradicate the bacteria effectively, both in the planktonic state and within a biofilm. Besides gentamicin, we also successfully demonstrate the applicability of the CAP-activated composite hydrogel loaded with other antimicrobial agents such as cetrimide and silver. This concept of a composite hydrogel is potentially adaptable to a range of therapeutics (such as antimicrobials, anticancer agents, and nanoparticles) and activatable using any dielectric barrier discharge CAP device.

Original language	English
Pages (from-to)	19989-19996
Journal	ACS Applied Materials and Interfaces
Volume	15
Issue number	16
Early online date	11 Apr 2023
DOIs	https://doi.org/10.1021/acsami.3c01208
Publication status	Published - 26 Apr 2023

Bibliographical note

Funding Information:
The authors would like to thank the UK EPSRC grant references EP/V00462X/1 and EP/R003939/1 and an EPSRC IAA award. B.L.P. would also like to thank the James Tudor Foundation for funding her research.

Funding

The authors would like to thank the UK EPSRC grant references EP/V00462X/1 and EP/R003939/1 and an EPSRC IAA award. B.L.P. would also like to thank the James Tudor Foundation for funding her research.

Keywords

antimicrobial
biofilms
cold atmospheric plasma
composite hydrogels
drug delivery
gentamicin
plasma jet
reactive oxygen and nitrogen species

ASJC Scopus subject areas

General Materials Science

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10.1021/acsami.3c01208Licence: CC BY

Cite this

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title = "Cold Atmospheric Plasma-Activated Composite Hydrogel for an Enhanced and On-Demand Delivery of Antimicrobials",

abstract = "We present the concept of a versatile drug-loaded composite hydrogel that can be activated using an argon-based cold atmospheric plasma (CAP) jet to deliver both a drug and CAP-generated molecules, concomitantly, in a tissue target. To demonstrate this concept, we utilized the antibiotic gentamicin that is encapsulated in sodium polyacrylate (PAA) particles, which are dispersed within a poly(vinyl alcohol) (PVA) hydrogel matrix. The final product is a gentamicin-PAA-PVA composite hydrogel suitable for an on-demand triggered release using CAP. We show that by activating using CAP, we can effectively release gentamicin from the hydrogel and also eradicate the bacteria effectively, both in the planktonic state and within a biofilm. Besides gentamicin, we also successfully demonstrate the applicability of the CAP-activated composite hydrogel loaded with other antimicrobial agents such as cetrimide and silver. This concept of a composite hydrogel is potentially adaptable to a range of therapeutics (such as antimicrobials, anticancer agents, and nanoparticles) and activatable using any dielectric barrier discharge CAP device.",

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author = "Nishtha Gaur and Patenall, {Bethany Lee} and Bhagirath Ghimire and Naing Thet and Jordan Gardiner and {Le Dore}, Krystal and Gordon Ramage and Bryn Short and Rachel Heylen and Craig Williams and Robert Short and Andrew Jenkins",

note = "Funding Information: The authors would like to thank the UK EPSRC grant references EP/V00462X/1 and EP/R003939/1 and an EPSRC IAA award. B.L.P. would also like to thank the James Tudor Foundation for funding her research. ",

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AU - Gaur, Nishtha

AU - Patenall, Bethany Lee

AU - Ghimire, Bhagirath

AU - Thet, Naing

AU - Gardiner, Jordan

AU - Le Dore, Krystal

AU - Ramage, Gordon

AU - Short, Bryn

AU - Heylen, Rachel

AU - Williams, Craig

AU - Short, Robert

AU - Jenkins, Andrew

N1 - Funding Information: The authors would like to thank the UK EPSRC grant references EP/V00462X/1 and EP/R003939/1 and an EPSRC IAA award. B.L.P. would also like to thank the James Tudor Foundation for funding her research.

PY - 2023/4/26

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N2 - We present the concept of a versatile drug-loaded composite hydrogel that can be activated using an argon-based cold atmospheric plasma (CAP) jet to deliver both a drug and CAP-generated molecules, concomitantly, in a tissue target. To demonstrate this concept, we utilized the antibiotic gentamicin that is encapsulated in sodium polyacrylate (PAA) particles, which are dispersed within a poly(vinyl alcohol) (PVA) hydrogel matrix. The final product is a gentamicin-PAA-PVA composite hydrogel suitable for an on-demand triggered release using CAP. We show that by activating using CAP, we can effectively release gentamicin from the hydrogel and also eradicate the bacteria effectively, both in the planktonic state and within a biofilm. Besides gentamicin, we also successfully demonstrate the applicability of the CAP-activated composite hydrogel loaded with other antimicrobial agents such as cetrimide and silver. This concept of a composite hydrogel is potentially adaptable to a range of therapeutics (such as antimicrobials, anticancer agents, and nanoparticles) and activatable using any dielectric barrier discharge CAP device.

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KW - plasma jet

KW - reactive oxygen and nitrogen species

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Cold Atmospheric Plasma-Activated Composite Hydrogel for an Enhanced and On-Demand Delivery of Antimicrobials

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Plasma-activated antimicrobial hydrogel therapy (PAHT) for combating infections in diabetic foot ulcers - Bath-Lancaster-Glasgow project

Smartwound-plasma

Cite this

Cold Atmospheric Plasma-Activated Composite Hydrogel for an Enhanced and On-Demand Delivery of Antimicrobials

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Projects

Plasma-activated antimicrobial hydrogel therapy (PAHT) for combating infections in diabetic foot ulcers - Bath-Lancaster-Glasgow project

Smartwound-plasma

Cite this