Cloning, purification, crystallization and preliminary crystallographic analysis of the human histone deacetylase sirtuin 1

Kenneth P Holbourn; Matthew D Lloyd; Andrew S Thompson; Michael D Threadgill; K Ravi Acharya

doi:10.1107/s1744309111003277

Cloning, purification, crystallization and preliminary crystallographic analysis of the human histone deacetylase sirtuin 1

Kenneth P Holbourn
, Matthew D Lloyd
, Andrew S Thompson
, Michael D Threadgill
, K Ravi Acharya

Department of Life Sciences

Research output: Contribution to journal › Article › peer-review

1 Citation (SciVal)

Abstract

Human sirtuin 1 is a member of the histone deacetylase family and is involved in cellular aging, tumourigenesis and cellular metabolism. Recombinant sirtuin 1 comprising residues 140-747 was crystallized using the hanging-drop vapour-diffusion method. The crystal diffracted to 3.4 A resolution and belonged to space group P622, with unit-cell parameters a = b = 203.1, c = 625.3 A, and is estimated to contain between six and 12 molecules per asymmetric unit.

Original language	English
Pages (from-to)	461-463
Number of pages	3
Journal	Acta Crystallographica Section F-Structural Biology and Crystallization Communications
Volume	67
Issue number	4
DOIs	https://doi.org/10.1107/s1744309111003277
Publication status	Published - Apr 2011

Keywords

sirtuin 1
histone deacetylases

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10.1107/s1744309111003277

Cite this

Cloning, purification, crystallization and preliminary crystallographic analysis of the human histone deacetylase sirtuin 1. / Holbourn, Kenneth P; Lloyd, Matthew D; Thompson, Andrew S et al.
In: Acta Crystallographica Section F-Structural Biology and Crystallization Communications, Vol. 67, No. 4, 04.2011, p. 461-463.

Research output: Contribution to journal › Article › peer-review

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AU - Acharya, K Ravi

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JF - Acta Crystallographica Section F-Structural Biology and Crystallization Communications

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ER -

Cloning, purification, crystallization and preliminary crystallographic analysis of the human histone deacetylase sirtuin 1

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