Cloning, purification, crystallization and preliminary crystallographic analysis of the human histone deacetylase sirtuin 1

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Abstract

Human sirtuin 1 is a member of the histone deacetylase family and is involved in cellular aging, tumourigenesis and cellular metabolism. Recombinant sirtuin 1 comprising residues 140-747 was crystallized using the hanging-drop vapour-diffusion method. The crystal diffracted to 3.4 A resolution and belonged to space group P622, with unit-cell parameters a = b = 203.1, c = 625.3 A, and is estimated to contain between six and 12 molecules per asymmetric unit.
Original languageEnglish
Pages (from-to)461-463
Number of pages3
JournalActa Crystallographica Section F-Structural Biology and Crystallization Communications
Volume67
Issue number4
DOIs
Publication statusPublished - Apr 2011

Keywords

  • sirtuin 1
  • histone deacetylases

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