TY - JOUR
T1 - Cloning, purification, crystallization and preliminary crystallographic analysis of the human histone deacetylase sirtuin 1
AU - Holbourn, Kenneth P
AU - Lloyd, Matthew D
AU - Thompson, Andrew S
AU - Threadgill, Michael D
AU - Acharya, K Ravi
PY - 2011/4
Y1 - 2011/4
N2 - Human sirtuin 1 is a member of the histone deacetylase family and is involved in cellular aging, tumourigenesis and cellular metabolism. Recombinant sirtuin 1 comprising residues 140-747 was crystallized using the hanging-drop vapour-diffusion method. The crystal diffracted to 3.4 A resolution and belonged to space group P622, with unit-cell parameters a = b = 203.1, c = 625.3 A, and is estimated to contain between six and 12 molecules per asymmetric unit.
AB - Human sirtuin 1 is a member of the histone deacetylase family and is involved in cellular aging, tumourigenesis and cellular metabolism. Recombinant sirtuin 1 comprising residues 140-747 was crystallized using the hanging-drop vapour-diffusion method. The crystal diffracted to 3.4 A resolution and belonged to space group P622, with unit-cell parameters a = b = 203.1, c = 625.3 A, and is estimated to contain between six and 12 molecules per asymmetric unit.
KW - sirtuin 1
KW - histone deacetylases
UR - http://www.scopus.com/inward/record.url?scp=79955100418&partnerID=8YFLogxK
UR - http://dx.doi.org/10.1107/s1744309111003277
U2 - 10.1107/s1744309111003277
DO - 10.1107/s1744309111003277
M3 - Article
SN - 1744-3091
VL - 67
SP - 461
EP - 463
JO - Acta Crystallographica Section F-Structural Biology and Crystallization Communications
JF - Acta Crystallographica Section F-Structural Biology and Crystallization Communications
IS - 4
ER -