Chromatin analysis of metabolic gene clusters in plants

Ancheng C. Huang, Hans-Wilhelm Nützmann

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Plant metabolic gene clusters consist of neighboring genes that are involved in the biosynthesis of secondary or specialized metabolites. The genes within clusters are typically co-regulated, share a common set of chromatin marks, and code for the biosynthesis enzymes of a single metabolic pathway. Here, we describe three essential protocols for the basic analysis of metabolic gene clusters at transcription, histone modification, and metabolite level. The protocols are specified to clusters in the Arabidopsis thaliana genome and are transferable to other plant species.

Original languageEnglish
Title of host publicationPlant Epigenetics and Epigenomics (2nd ed.)
EditorsC. Spillane, P. McKeown
Place of PublicationNew York, U. S. A.
PublisherHumana Press
Pages129-146
ISBN (Electronic)9781071601792
ISBN (Print)9781071601785
DOIs
Publication statusPublished - 23 Feb 2020

Publication series

NameMethods in Molecular Biology
Volume2093

Cite this

Huang, A. C., & Nützmann, H-W. (2020). Chromatin analysis of metabolic gene clusters in plants. In C. Spillane, & P. McKeown (Eds.), Plant Epigenetics and Epigenomics (2nd ed.) (pp. 129-146). (Methods in Molecular Biology; Vol. 2093). Humana Press. https://doi.org/10.1007/978-1-0716-0179-2_10