Choosing a cloning vector

Research output: Chapter in Book/Report/Conference proceedingChapter

20 Citations (Scopus)

Abstract

Since the construction of the first generation of general cloning vectors in the early 1970s, the number of plasmids created has increased to an almost countless number. Thus, a critical decision facing today’s investigator is that of which plasmid to use in a particular project? Despite the bewildering choice of commercial and other available vectors, the choice of which cloning vector to use can be decided by applying a small number of criteria: insert size, copy number, incompatibility, selectable marker, cloning sites, and specialized vector functions. Several of these criteria are dependent on each other. This chapter discusses these criteria in the context of choosing a plasmid for use as a cloning vector and Table 1 displays the features of some commonly used cloning vectors.
Original languageEnglish
Title of host publicationE-coli Plasmid Vectors
Subtitle of host publicationMethods and Applications
EditorsNicola Casali, Andrew Preston
PublisherHumana Press
Pages19-26
Number of pages8
ISBN (Electronic)9781592594092
ISBN (Print)9781588291516
DOIs
Publication statusPublished - 1 Jul 2003

Publication series

NameMethods in Molecular Biology
Volume235

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Fingerprint Dive into the research topics of 'Choosing a cloning vector'. Together they form a unique fingerprint.

Cite this