Cholesterol biosensor based on inkjet-printed Prussian blue nanoparticle-modified screen-printed electrodes

Stefano Cinti; Fabiana Arduini; Danila Moscone; Giuseppe Palleschi; Laura Gonzalez-Macia; Anthony J. Killard

doi:10.1016/j.snb.2015.06.054

Cholesterol biosensor based on inkjet-printed Prussian blue nanoparticle-modified screen-printed electrodes

Stefano Cinti
, Fabiana Arduini
, Danila Moscone
, Giuseppe Palleschi
, Laura Gonzalez-Macia
, Anthony J. Killard

Università degli Studi di Roma Tor Vergata
Department of Biological
University of the West of England

Research output: Contribution to journal › Article › peer-review

68 Citations (SciVal)

Abstract

Here we describe the construction and optimization of a cholesterol biosensor based on screen-printed electrodes (SPEs) modified with inkjet-printed Prussian blue nanoparticles (PBNPs). The deposition of PBNPs using inkjet printing led to the highly facile fabrication of sensors with excellent sensitivity and reproducibility for the measurement of H₂O₂. Further integration of the sensor with a microfabricated low volume (4 μL) sample cell allowed the measurement of cholesterol in serum with the addition of cholesterol oxidase. The biosensor exhibited a sensitivity to cholesterol of 2.1 μA/mM cm² (r² = 0.97, n = 5) and was linear in the range of 0-15 mM.

Original language	English
Pages (from-to)	187-190
Number of pages	4
Journal	Sensors and Actuators, B: Chemical
Volume	221
DOIs	https://doi.org/10.1016/j.snb.2015.06.054
Publication status	Published - 31 Dec 2015

Keywords

Biosensor
Cholesterol
Encapsulation
Inkjet-printing
Prussian blue nanoparticles
Screen-printed electrodes

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Instrumentation
Condensed Matter Physics
Surfaces, Coatings and Films
Metals and Alloys
Electrical and Electronic Engineering
Materials Chemistry

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10.1016/j.snb.2015.06.054

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title = "Cholesterol biosensor based on inkjet-printed Prussian blue nanoparticle-modified screen-printed electrodes",

abstract = "Here we describe the construction and optimization of a cholesterol biosensor based on screen-printed electrodes (SPEs) modified with inkjet-printed Prussian blue nanoparticles (PBNPs). The deposition of PBNPs using inkjet printing led to the highly facile fabrication of sensors with excellent sensitivity and reproducibility for the measurement of H2O2. Further integration of the sensor with a microfabricated low volume (4 μL) sample cell allowed the measurement of cholesterol in serum with the addition of cholesterol oxidase. The biosensor exhibited a sensitivity to cholesterol of 2.1 μA/mM cm2 (r2 = 0.97, n = 5) and was linear in the range of 0-15 mM.",

keywords = "Biosensor, Cholesterol, Encapsulation, Inkjet-printing, Prussian blue nanoparticles, Screen-printed electrodes",

author = "Stefano Cinti and Fabiana Arduini and Danila Moscone and Giuseppe Palleschi and Laura Gonzalez-Macia and Killard, \{Anthony J.\}",

year = "2015",

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doi = "10.1016/j.snb.2015.06.054",

language = "English",

volume = "221",

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journal = "Sensors and Actuators, B: Chemical",

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TY - JOUR

T1 - Cholesterol biosensor based on inkjet-printed Prussian blue nanoparticle-modified screen-printed electrodes

AU - Cinti, Stefano

AU - Arduini, Fabiana

AU - Moscone, Danila

AU - Palleschi, Giuseppe

AU - Gonzalez-Macia, Laura

AU - Killard, Anthony J.

PY - 2015/12/31

Y1 - 2015/12/31

N2 - Here we describe the construction and optimization of a cholesterol biosensor based on screen-printed electrodes (SPEs) modified with inkjet-printed Prussian blue nanoparticles (PBNPs). The deposition of PBNPs using inkjet printing led to the highly facile fabrication of sensors with excellent sensitivity and reproducibility for the measurement of H2O2. Further integration of the sensor with a microfabricated low volume (4 μL) sample cell allowed the measurement of cholesterol in serum with the addition of cholesterol oxidase. The biosensor exhibited a sensitivity to cholesterol of 2.1 μA/mM cm2 (r2 = 0.97, n = 5) and was linear in the range of 0-15 mM.

AB - Here we describe the construction and optimization of a cholesterol biosensor based on screen-printed electrodes (SPEs) modified with inkjet-printed Prussian blue nanoparticles (PBNPs). The deposition of PBNPs using inkjet printing led to the highly facile fabrication of sensors with excellent sensitivity and reproducibility for the measurement of H2O2. Further integration of the sensor with a microfabricated low volume (4 μL) sample cell allowed the measurement of cholesterol in serum with the addition of cholesterol oxidase. The biosensor exhibited a sensitivity to cholesterol of 2.1 μA/mM cm2 (r2 = 0.97, n = 5) and was linear in the range of 0-15 mM.

KW - Biosensor

KW - Cholesterol

KW - Encapsulation

KW - Inkjet-printing

KW - Prussian blue nanoparticles

KW - Screen-printed electrodes

UR - https://www.scopus.com/pages/publications/84936868410

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DO - 10.1016/j.snb.2015.06.054

M3 - Article

AN - SCOPUS:84936868410

SN - 0925-4005

VL - 221

SP - 187

EP - 190

JO - Sensors and Actuators, B: Chemical

JF - Sensors and Actuators, B: Chemical

ER -

Cholesterol biosensor based on inkjet-printed Prussian blue nanoparticle-modified screen-printed electrodes

Abstract

Keywords

ASJC Scopus subject areas

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