Chlorophyll interference in microalgal lipid quantification through the Bligh and Dyer method

S. Archanaa, Sandhya Moise, G. K. Suraishkumar

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)

Abstract

The screening for high lipid producing microalgae is a crucial step to enable large scale cultivation of suitable microalgae as a means of alternative energy. The Bligh and Dyer method has been traditionally used for the determination of biofuel-related lipids. But, we found that this method also extracts chlorophyll, to result in significant over-estimation of biofuel-related lipid measures. In this work, we present and characterize a method to obviate the chlorophyll interference to biofuel-related lipid determination.

Original languageEnglish
Pages (from-to)805-808
Number of pages4
JournalBiomass and Bioenergy
Volume46
DOIs
Publication statusPublished - 1 Nov 2012

Keywords

  • Biofuel-related lipid extraction
  • Chlorophyll contamination
  • DMSO
  • Microalgae
  • Neutral lipids

ASJC Scopus subject areas

  • Forestry
  • Renewable Energy, Sustainability and the Environment
  • Agronomy and Crop Science
  • Waste Management and Disposal

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