Chlorophyll interference in microalgal lipid quantification through the Bligh and Dyer method

Sandhya Moise; S. Archanaa; G. K. Suraishkumar

doi:10.1016/j.biombioe.2012.07.002

Chlorophyll interference in microalgal lipid quantification through the Bligh and Dyer method

Sandhya Moise, S. Archanaa, G. K. Suraishkumar

Department of Chemical Engineering

Indian Institute of Technology

Research output: Contribution to journal › Article › peer-review

31 Citations (SciVal)

Abstract

The screening for high lipid producing microalgae is a crucial step to enable large scale cultivation of suitable microalgae as a means of alternative energy. The Bligh and Dyer method has been traditionally used for the determination of biofuel-related lipids. But, we found that this method also extracts chlorophyll, to result in significant over-estimation of biofuel-related lipid measures. In this work, we present and characterize a method to obviate the chlorophyll interference to biofuel-related lipid determination.

Original language	English
Pages (from-to)	805-808
Number of pages	4
Journal	Biomass and Bioenergy
Volume	46
DOIs	https://doi.org/10.1016/j.biombioe.2012.07.002
Publication status	Published - 1 Nov 2012

Keywords

Biofuel-related lipid extraction
Chlorophyll contamination
DMSO
Microalgae
Neutral lipids

ASJC Scopus subject areas

Forestry
Renewable Energy, Sustainability and the Environment
Agronomy and Crop Science
Waste Management and Disposal

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.biombioe.2012.07.002

Cite this

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abstract = "The screening for high lipid producing microalgae is a crucial step to enable large scale cultivation of suitable microalgae as a means of alternative energy. The Bligh and Dyer method has been traditionally used for the determination of biofuel-related lipids. But, we found that this method also extracts chlorophyll, to result in significant over-estimation of biofuel-related lipid measures. In this work, we present and characterize a method to obviate the chlorophyll interference to biofuel-related lipid determination.",

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AU - Moise, Sandhya

AU - Archanaa, S.

AU - Suraishkumar, G. K.

PY - 2012/11/1

Y1 - 2012/11/1

N2 - The screening for high lipid producing microalgae is a crucial step to enable large scale cultivation of suitable microalgae as a means of alternative energy. The Bligh and Dyer method has been traditionally used for the determination of biofuel-related lipids. But, we found that this method also extracts chlorophyll, to result in significant over-estimation of biofuel-related lipid measures. In this work, we present and characterize a method to obviate the chlorophyll interference to biofuel-related lipid determination.

AB - The screening for high lipid producing microalgae is a crucial step to enable large scale cultivation of suitable microalgae as a means of alternative energy. The Bligh and Dyer method has been traditionally used for the determination of biofuel-related lipids. But, we found that this method also extracts chlorophyll, to result in significant over-estimation of biofuel-related lipid measures. In this work, we present and characterize a method to obviate the chlorophyll interference to biofuel-related lipid determination.

KW - Biofuel-related lipid extraction

KW - Chlorophyll contamination

KW - DMSO

KW - Microalgae

KW - Neutral lipids

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SN - 0961-9534

VL - 46

SP - 805

EP - 808

JO - Biomass and Bioenergy

JF - Biomass and Bioenergy

ER -

Chlorophyll interference in microalgal lipid quantification through the Bligh and Dyer method

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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