The screening for high lipid producing microalgae is a crucial step to enable large scale cultivation of suitable microalgae as a means of alternative energy. The Bligh and Dyer method has been traditionally used for the determination of biofuel-related lipids. But, we found that this method also extracts chlorophyll, to result in significant over-estimation of biofuel-related lipid measures. In this work, we present and characterize a method to obviate the chlorophyll interference to biofuel-related lipid determination.
- Biofuel-related lipid extraction
- Chlorophyll contamination
- Neutral lipids
ASJC Scopus subject areas
- Renewable Energy, Sustainability and the Environment
- Agronomy and Crop Science
- Waste Management and Disposal