Cheno-, urso- and deoxycholic acid spermine conjugates: Relative binding affinities for calf thymus DNA

Ian S Blagbrough; D Al-Hadithi; A J Geall

doi:10.1016/S0040-4020(00)00265-9

Cheno-, urso- and deoxycholic acid spermine conjugates: Relative binding affinities for calf thymus DNA

Ian S Blagbrough, D Al-Hadithi, A J Geall

Department of Life Sciences

Research output: Contribution to journal › Article › peer-review

38 Citations (SciVal)

Abstract

Cationic lipid polyamine amides (cholan-24-amides) have been prepared from chenodeoxycholic (3 alpha,7 alpha-dihydroxy), ursodeoxycholic (3 alpha,7 beta-dihydroxy), and deoxycholic (3 alpha,12 alpha-dihydroxy) bile acids (5 beta-cholanes) by acylation of tri-Boc protected spermine. Their relative binding affinities for calf thymus DNA were determined using an ethidium bromide displacement assay. These lipopolyamine amides are synthetic vectors for non-viral gene delivery and models for lipoplex formation with respect to lipofection, a key first step in gene therapy.

Original language	English
Pages (from-to)	3439-3447
Number of pages	9
Journal	Tetrahedron
Volume	56
Issue number	21
DOIs	https://doi.org/10.1016/S0040-4020(00)00265-9
Publication status	Published - 2000

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title = "Cheno-, urso- and deoxycholic acid spermine conjugates: Relative binding affinities for calf thymus DNA",

abstract = "Cationic lipid polyamine amides (cholan-24-amides) have been prepared from chenodeoxycholic (3 alpha,7 alpha-dihydroxy), ursodeoxycholic (3 alpha,7 beta-dihydroxy), and deoxycholic (3 alpha,12 alpha-dihydroxy) bile acids (5 beta-cholanes) by acylation of tri-Boc protected spermine. Their relative binding affinities for calf thymus DNA were determined using an ethidium bromide displacement assay. These lipopolyamine amides are synthetic vectors for non-viral gene delivery and models for lipoplex formation with respect to lipofection, a key first step in gene therapy.",

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T1 - Cheno-, urso- and deoxycholic acid spermine conjugates: Relative binding affinities for calf thymus DNA

AU - Blagbrough, Ian S

AU - Al-Hadithi, D

AU - Geall, A J

PY - 2000

Y1 - 2000

N2 - Cationic lipid polyamine amides (cholan-24-amides) have been prepared from chenodeoxycholic (3 alpha,7 alpha-dihydroxy), ursodeoxycholic (3 alpha,7 beta-dihydroxy), and deoxycholic (3 alpha,12 alpha-dihydroxy) bile acids (5 beta-cholanes) by acylation of tri-Boc protected spermine. Their relative binding affinities for calf thymus DNA were determined using an ethidium bromide displacement assay. These lipopolyamine amides are synthetic vectors for non-viral gene delivery and models for lipoplex formation with respect to lipofection, a key first step in gene therapy.

AB - Cationic lipid polyamine amides (cholan-24-amides) have been prepared from chenodeoxycholic (3 alpha,7 alpha-dihydroxy), ursodeoxycholic (3 alpha,7 beta-dihydroxy), and deoxycholic (3 alpha,12 alpha-dihydroxy) bile acids (5 beta-cholanes) by acylation of tri-Boc protected spermine. Their relative binding affinities for calf thymus DNA were determined using an ethidium bromide displacement assay. These lipopolyamine amides are synthetic vectors for non-viral gene delivery and models for lipoplex formation with respect to lipofection, a key first step in gene therapy.

UR - http://dx.doi.org/10.1016/S0040-4020(00)00265-9

UR - https://www.scopus.com/pages/publications/0034685930

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DO - 10.1016/S0040-4020(00)00265-9

M3 - Article

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VL - 56

SP - 3439

EP - 3447

JO - Tetrahedron

JF - Tetrahedron

IS - 21

ER -

Cheno-, urso- and deoxycholic acid spermine conjugates: Relative binding affinities for calf thymus DNA

Abstract

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