TY - JOUR
T1 - Cheno-, urso- and deoxycholic acid spermine conjugates: Relative binding affinities for calf thymus DNA
AU - Blagbrough, Ian S
AU - Al-Hadithi, D
AU - Geall, A J
PY - 2000
Y1 - 2000
N2 - Cationic lipid polyamine amides (cholan-24-amides) have been prepared from chenodeoxycholic (3 alpha,7 alpha-dihydroxy), ursodeoxycholic (3 alpha,7 beta-dihydroxy), and deoxycholic (3 alpha,12 alpha-dihydroxy) bile acids (5 beta-cholanes) by acylation of tri-Boc protected spermine. Their relative binding affinities for calf thymus DNA were determined using an ethidium bromide displacement assay. These lipopolyamine amides are synthetic vectors for non-viral gene delivery and models for lipoplex formation with respect to lipofection, a key first step in gene therapy.
AB - Cationic lipid polyamine amides (cholan-24-amides) have been prepared from chenodeoxycholic (3 alpha,7 alpha-dihydroxy), ursodeoxycholic (3 alpha,7 beta-dihydroxy), and deoxycholic (3 alpha,12 alpha-dihydroxy) bile acids (5 beta-cholanes) by acylation of tri-Boc protected spermine. Their relative binding affinities for calf thymus DNA were determined using an ethidium bromide displacement assay. These lipopolyamine amides are synthetic vectors for non-viral gene delivery and models for lipoplex formation with respect to lipofection, a key first step in gene therapy.
UR - http://dx.doi.org/10.1016/S0040-4020(00)00265-9
U2 - 10.1016/S0040-4020(00)00265-9
DO - 10.1016/S0040-4020(00)00265-9
M3 - Article
SN - 0040-4020
VL - 56
SP - 3439
EP - 3447
JO - Tetrahedron
JF - Tetrahedron
IS - 21
ER -